The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica

Ziborov, Vadim S.; Pleshakova, Tatyana O.; Shumov, Ivan D.; Kozlov, Andrey F.; Valueva, Anastasia A.; Ivanova, Irina A.; Ershova, Maria O.; Larionov, Dmitry I.; Evdokimov, A. N.; Tatur, Vadim Yu.; Aleshko, A. I.; Sakharov, K. Yu.; Dolgoborodov, A. Yu.; Фортов, В. Е.; Archakov, Alexander I.; Ivanov, Yuri D.

doi:10.3390/app112411677

Cited by 7 publications

(9 citation statements)

References 69 publications

(121 reference statements)

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“…This change can well occur in the course of the 40-min-long incubation of the enzyme sample near the heat exchanger with stopped glycerol flow [19]. The change in the hydration shell of HRP enzyme molecules can influence their interactions with each other [46] as well as with the mica substrate surface [47,48]. This effect could be associated with long-lived perturbations resulting from formation of nanobubble clusters [49].…”

Section: Discussionmentioning

confidence: 99%

AFM Study of the Influence of Stopped Glycerol Flow in a Coil on Peroxidase Enzyme

Ivanov,

Shumov,

Kozlov

et al. 2024

Preprint

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Glycerol represents a useful functional component of heat-transfer fluids. Glycerol is known to be triboelectrically active. We report how the flow of glycerol, stopped in a cylindrical coiled pipe, influences horseradish peroxidase (HRP) enzyme. Namely, glycerol flow was pumped through the coil and then stopped. After that, we incubated the 0.1 µM solution of HRP near the side of the coil, and then adsorbed HRP onto mica. This operation markedly increased the HRP aggregation on mica in comparison with the control sample. We explain this phenomenon by the influence of triboelectrically induced electromagnetic fields. Our results can be useful in the development of flow-based systems for biosensors and bioreactors.

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Section: Discussionmentioning

confidence: 99%

AFM Study of the Influence of Stopped Glycerol Flow in a Coil on Peroxidase Enzyme

Ivanov,

Shumov,

Kozlov

et al. 2024

Preprint

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show abstract

“…We must emphasize that in enzyme adsorption studies, enzyme-enzyme, enzymesubstrate, and enzyme-solvent interactions should be taken into consideration [48]. The combination of these interactions will determine the aggregation state of the adsorbed enzyme in each particular case.…”

Section: Discussionmentioning

confidence: 99%

“…This change can also occur in the course of the 40-min-long incubation of the enzyme sample near the heat exchanger with stopped glycerol flow [19]. The change in the outer hydration shells of HRP enzyme globules can influence their interactions with each other [61], as well as with the surface of the mica substrate [48,62]. This effect could be associated with long-lived perturbations resulting from the formation of nanobubble clusters [63].…”

Section: Discussionmentioning

confidence: 99%

Atomic Force Microscopy Study of the Long-Term Effect of the Glycerol Flow, Stopped in a Coiled Heat Exchanger, on Horseradish Peroxidase

Ivanov,

Shumov,

Kozlov

et al. 2024

Micromachines

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Glycerol is employed as a functional component of heat-transfer fluids, which are of use in both bioreactors and various biosensor devices. At the same time, flowing glycerol was reported to cause considerable triboelectric effects. Herein, by using atomic force microscopy (AFM), we have revealed the long-term effect of glycerol flow, stopped in a ground-shielded coiled heat exchanger, on horseradish peroxidase (HRP) adsorption on mica. Namely, the solution of HRP was incubated in the vicinity of the side of the cylindrical coil with stopped glycerol flow, and then HRP was adsorbed from this solution onto a mica substrate. This incubation has been found to markedly increase the content of aggregated enzyme on mica—as compared with the control enzyme sample. We explain the phenomenon observed by the influence of triboelectrically induced electromagnetic fields of non-trivial topology. The results reported should be further considered in the development of flow-based heat exchangers of biosensors and bioreactors intended for operation with enzymes.

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“…It is to be noted that pH directly influences the electrostatic mechanism of enzyme adsorption [79]. In general, enzyme adsorption represents a complex process, which involves the interactions of enzyme macromolecules with each other (that is, enzyme aggregation), with the surface and with the solvent [80]. Based on these considerations, we have conducted our experiments at room temperature and pH 7.4 in order to confirm that the observed effects on the enzyme aggregation and activity are only caused by the field generated in the experimental setup.…”

Section: Discussionmentioning

confidence: 99%

Glycerol Flow through a Shielded Coil Induces Aggregation and Activity Enhancement of Horseradish Peroxidase

et al. 2023

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Glycerol has found its applications as a heat-transfer fluid in heat exchangers, and as a component of functional liquids in biosensor analysis. Flowing non-aqueous fluids are known to be able to induce electromagnetic fields due to the triboelectric effect. These triboelectrically generated electromagnetic fields can affect biological macromolecules. Horseradish peroxidase (HRP) is widely employed as a convenient model object for studying how external electric, magnetic, and electromagnetic fields affect enzymes. Herein, we have studied whether the flow of glycerol in a ground-shielded cylindrical coil affects the HRP enzyme incubated at a 2 cm distance near the coil’s side. Atomic force microscopy (AFM) has been employed in order to study the effect of glycerol flow on HRP at the nanoscale. An increased aggregation of HRP on mica has been observed after the incubation of the enzyme near the coil. Moreover, the enzymatic activity of HRP has also been affected. The results reported that their application can be found in biotechnology, food technology and life sciences applications, considering the development of triboelectric generators, enzyme-based biosensors and bioreactors with surface-immobilized enzymes. Our work can also be of interest for scientists studying triboelectric phenomena, representing one more step toward understanding the mechanism of the indirect action of the flow of a dielectric liquid on biological macromolecules.

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The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica

Cited by 7 publications

References 69 publications

AFM Study of the Influence of Stopped Glycerol Flow in a Coil on Peroxidase Enzyme

AFM Study of the Influence of Stopped Glycerol Flow in a Coil on Peroxidase Enzyme

Atomic Force Microscopy Study of the Long-Term Effect of the Glycerol Flow, Stopped in a Coiled Heat Exchanger, on Horseradish Peroxidase

Glycerol Flow through a Shielded Coil Induces Aggregation and Activity Enhancement of Horseradish Peroxidase

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