A WRKY transcription factor, FtWRKY46, from Tartary buckwheat improves salt tolerance in transgenic Arabidopsis thaliana

Lv, Bingbing; Wu, Qi; Wang, Anhu; Li, Qi; Dong, Qixin; Yang, Jingjing; Zhao, Huihui; Wang, Xiaoli; Chen, Hui; Li, Chenglei

doi:10.1016/j.plaphy.2019.12.004

Cited by 43 publications

(25 citation statements)

References 49 publications

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“…Moreover, the adult transgenic lines showed a distinct resistance to water deficit with a higher survival rate in comparison with WT plants. These results were also confirmed in other plants, such as FtWRKY46 in Tartary buckwheat [ 6 ] and HbWRKY82 in Hevea brasiliensis . In addition, overexpression of ZmWRKY17 resulted in decreased ABA sensitivity through regulating the expression of several well-known stress/ABA-responsive genes in Arabidopsis [ 35 ].…”

Section: Discussionsupporting

confidence: 72%

“…When plants are exposed to stress conditions, the transcription factors (TFs) act as central regulators by binding to specific cis -acting elements in the promoter regions to activate downstream gene expression, signal transduction, and adaptation networks [ 5 ]. To date, a wide range of TFs families in plants, including WRKY, NAC, MYB, and bHLH, have been functionally identified and elucidated to participate in abiotic stress in plants [ 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The modulation of the transcription of downstream target genes can be regulated by WRKY TFs via specifically binding to the W-box elements [(T)TGAC(C/T)] cis -elements [ 12 ]. Growing evidence has proved that WRKY proteins are involved in plant responses to a broad spectrum of abiotic stresses, such as drought, cold, and salt [ 6 , 14 , 15 ]. For instance, AtWRKY21 , AtWRKY46 , AtWRKY54 , and AtWRKY70 in Arabidopsis have been reported to regulate osmotic stress [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

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The WRKY gene family, which is one of the largest transcription factor (TF) families, plays an important role in numerous aspects of plant growth and development, especially in various stress responses. However, the functional roles of the WRKY gene family in loquat are relatively unknown. In this study, a novel WRKY gene, EjWRKY17, was characterized from Eriobotrya japonica, which was significantly upregulated in leaves by melatonin treatment during drought stress. The EjWRKY17 protein, belonging to group II of the WRKY family, was localized in the nucleus. The results indicated that overexpression of EjWRKY17 increased cotyledon greening and root elongation in transgenic Arabidopsis lines under abscisic acid (ABA) treatment. Meanwhile, overexpression of EjWRKY17 led to enhanced drought tolerance in transgenic lines, which was supported by the lower water loss, limited electrolyte leakage, and lower levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Further investigations showed that overexpression of EjWRKY17 promoted ABA-mediated stomatal closure and remarkably up-regulated ABA biosynthesis and stress-related gene expression in transgenic lines under drought stress. Overall, our findings reveal that EjWRKY17 possibly acts as a positive regulator in ABA-regulated drought tolerance.

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Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

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“…Most of them function as transcriptional activators and enhance stress tolerance by regulating stress-responsive gene expression. For example, Tartary buckwheat FtWRKY46 , chrysanthemum DgWRKY4 and DgWRKY5 , and cotton GarWRKY5 improve tolerance to salt stress [ 6 , 33 , 34 , 35 ]. Similarly, our previous study reported that ThWRKY4 enhances salt tolerance through the regulation of downstream target genes [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

“…Transgenic plants overexpressing cotton GhWRKY6 -like gene or sweet potato IbWRKY2 display enhanced salt tolerance by regulating the ABA signaling pathway and ROS scavenging [ 30 , 32 ]. Recently, the expression of FtWRKY46 from buckwheat, DgWRKY4 and DgWRKY5 from chrysanthemum, and GarWRKY5 from cotton in transgenic plants was shown to confer enhanced tolerance to salt stress by modulating ROS clearance, antioxidant enzyme activity, and stress-related gene expression [ 6 , 33 , 34 , 35 ]. Although many studies have reported that WRKY gene s are involved in different types of abiotic stress by enhancing downstream stress-responsive gene expression, it is not clear which TFs can regulate WRKY s in response to salt stress.…”

Section: Introductionmentioning

confidence: 99%

ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating ThWRKY4

Sun

Wang

et al. 2021

IJMS

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Heat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of ThHSFA1-ThWRKY4 in Tamarix hispida and their functions and regulatory mechanisms in salt tolerance. ThHSFA1 protein acts as an upstream regulator that can directly activate ThWRKY4 expression by binding to the heat shock element (HSE) of the ThWRKY4 promoter using yeast one-hybrid (Y1H), chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays. ThHSFA1 and ThWRKY4 expression was significantly induced by salt stress and abscisic acid (ABA) treatment in the roots and leaves of T. hispida. ThHSFA1 is a nuclear-localized protein with transactivation activity at the C-terminus. Compared to nontransgenic plants, transgenic plants overexpressing ThHSFA1 displayed enhanced salt tolerance and exhibited reduced reactive oxygen species (ROS) levels and increased antioxidant enzyme activity levels under salt stress. Therefore, we further concluded that ThHSFA1 mediated the regulation of ThWRKY4 in response to salt stress in T. hispida.

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Transcriptional Regulation of Gene Expression in Plant Abiotic Stress Response

Chaffai,

Ganesan,

Cherif

2024

Plant Adaptation to Abiotic Stress: From Signaling Pathways and Microbiomes to Molecular Mechanisms

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A WRKY transcription factor, FtWRKY46, from Tartary buckwheat improves salt tolerance in transgenic Arabidopsis thaliana

Cited by 43 publications

References 49 publications

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating ThWRKY4

Transcriptional Regulation of Gene Expression in Plant Abiotic Stress Response

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