A zinc finger transcription factor, ZicL, is a direct activator of<i>Brachyury</i>in the notochord specification of<i>Ciona intestinalis</i>

Yagi, Keisuke; Satou, Yutaka; Satoh, Nori

doi:10.1242/dev.01011

Cited by 92 publications

(90 citation statements)

References 38 publications

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“…Proteins were purified basically as described by Yagi et al (Yagi et al, 2004). SELEX experiments were performed as described previously (Senger et al, 2004) and details can be provided on request.…”

Section: Gene Expression Time Course Profilingmentioning

confidence: 99%

Identification of direct T-box target genes in the developing zebrafish mesoderm

et al. 2009

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The zebrafish genes spadetail (spt) and no tail (ntl) encode T-box transcription factors that are important for early mesoderm development. Although much has been done to characterize these genes, the identity and location of target regulatory elements remain largely unknown. Here, we survey the genome for downstream target genes of the Spt and Ntl T-box transcription factors. We find evidence for extensive additive interactions towards gene activation and limited evidence for combinatorial and antagonistic interactions between the two factors. Using in vitro binding selection assays to define Spt-and Ntl-binding motifs, we searched for target regulatory sequence via a combination of binding motif searches and comparative genomics. We identified regulatory elements for tbx6 and deltaD, and, using chromatin immunoprecipitation, in vitro DNA binding assays and transgenic methods, we provide evidence that both are directly regulated by T-box transcription factors. We also find that deltaD is directly activated by T-box factors in the tail bud, where it has been implicated in starting the segmentation clock, suggesting that spt and ntl act upstream of this process.

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Section: Gene Expression Time Course Profilingmentioning

confidence: 99%

Identification of direct T-box target genes in the developing zebrafish mesoderm

et al. 2009

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“…Briefly, nuclear localization of maternal ␤-catenin in endodermal precursors before the 32-cell stage occurs in response to the FGF signal emanating from the endoderm and, in turn, directly activates expression of FoxD, a gene encoding a wingedhelix transcription factor required for induction of both the primary and secondary notochord lineages (Imai et al, 2002a). In primary notochord precursors, the zinc finger transcription factor ZicL acts downstream of FoxD and binds sites in the Brachyury enhancer required for its activation (Yagi et al, 2004a). Suppressor of Hairless [Su(H)] also binds to the Brachyury promoter, activating expression .…”

Section: Larval Tissues Notochordmentioning

confidence: 99%

Ciona intestinalis: Chordate development made simple

2005

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Thanks to their transparent and rapidly developing mosaic embryos, ascidians (or sea squirts) have been a model system for embryological studies for over a century. Recently, ascidians have entered the postgenomic era, with the sequencing of the Ciona intestinalis genome and the accumulation of molecular resources that rival those available for fruit flies and mice. One strength of ascidians as a model system is their close similarity to vertebrates. Literature reporting molecular homologies between vertebrate and ascidian tissues has flourished over the past 15 years, since the first ascidian genes were cloned. However, it should not be forgotten that ascidians diverged from the lineage leading to vertebrates over 500 million years ago. Here, we review the main similarities and differences so far identified, at the molecular level, between ascidian and vertebrate tissues and discuss the evolution of the compact ascidian genome.

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“…Together, these observations provide a mechanistic cis-regulatory explanation to the results of our misexpression assay, as well as to previous results showing that overexpression of Ci-Macho1 is sufficient to induce ectopic expression of Ci-Tbx6b (Yagi et al, 2004a) and that, likewise, Hr-Macho-1 is able to ectopically induce Hr-Tbx6, among other muscle genes (Sawada et al, 2005). It is noteworthy that in Ciona, Ci-ZicL cooperates with Ci-Macho1 to promote muscle development (Yagi et al, 2005); this zygotic zinc-finger transcription factor is related to Ci-Macho1 and recognizes a similar consensus binding site in vitro (Yagi et al, 2004b). Interestingly, one of the three Ci-Macho1-binding sites that we have characterized in the Ci-Tbx6b CRM, namely site 'C', contains permutations of the published ZicL consensus site that are compatible with binding in vitro (Yagi et al, 2004b).…”

Section: Spatial and Temporal Heterogeneity Of The Embryonic Territormentioning

confidence: 84%

“…It is noteworthy that in Ciona, Ci-ZicL cooperates with Ci-Macho1 to promote muscle development (Yagi et al, 2005); this zygotic zinc-finger transcription factor is related to Ci-Macho1 and recognizes a similar consensus binding site in vitro (Yagi et al, 2004b). Interestingly, one of the three Ci-Macho1-binding sites that we have characterized in the Ci-Tbx6b CRM, namely site 'C', contains permutations of the published ZicL consensus site that are compatible with binding in vitro (Yagi et al, 2004b). If this site is bound in vivo by either transcription factor, then this would explain the observation that Ci-Tbx6b is still weakly expressed in Ci-Macho1 morphant embryos, whereas its expression is no longer detectable in Ci-Macho1 and Ci-ZicL double-morphants (Yagi et al, 2005).…”

Section: Spatial and Temporal Heterogeneity Of The Embryonic Territormentioning

confidence: 99%

Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis

Kugler¹,

Gazdoiu²,

Oda-Ishii³

et al. 2010

Development

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SummaryFor over a century, muscle formation in the ascidian embryo has been representative of 'mosaic' development. The molecular basis of muscle-fate predetermination has been partly elucidated with the discovery of Macho1, a maternal zinc-finger transcription factor necessary and sufficient for primary muscle development, and of its transcriptional intermediaries Tbx6b and Tbx6c. However, the molecular mechanisms by which the maternal information is decoded by cis-regulatory modules (CRMs) associated with muscle transcription factor and structural genes, and the ways by which a seamless transition from maternal to zygotic transcription is ensured, are still mostly unclear. By combining misexpression assays with CRM analyses, we have identified the mechanisms through which Ciona Macho1 (Ci-Macho1) initiates expression of Ci-Tbx6b and Ci-Tbx6c, and we have unveiled the cross-regulatory interactions between the latter transcription factors. Knowledge acquired from the analysis of the Ci-Tbx6b CRM facilitated both the identification of a related CRM in the Ci-Tbx6c locus and the characterization of two CRMs associated with the structural muscle gene fibrillar collagen 1 (CiFCol1). We use these representative examples to reconstruct how compact CRMs orchestrate the muscle developmental program from pre-localized ooplasmic determinants to differentiated larval muscle in ascidian embryos.

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A zinc finger transcription factor, ZicL, is a direct activator ofBrachyuryin the notochord specification ofCiona intestinalis

Abstract: SummaryA zinc finger transcription factor, ZicL, is a direct activator of Brachyury in the notochord specification of Ciona intestinalis

Cited by 92 publications

References 38 publications

Identification of direct T-box target genes in the developing zebrafish mesoderm

Identification of direct T-box target genes in the developing zebrafish mesoderm

Ciona intestinalis: Chordate development made simple

Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis

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