A Zn‐Doped CuO Nanocomposite Shows Enhanced Antibiofilm and Antibacterial Activities Against Streptococcus Mutans Compared to Nanosized CuO

Abstract

Zinc‐doped copper oxide and copper oxide nanoparticles (NPs) are synthesized and deposited on artificial teeth by sonic irradiation, and the ability of these coatings to restrict biofilm formation by Streptococcus mutans is examined. The CuO and Zn:CuO NP‐coated teeth show significant reductions in biofilm formation of 70% and 88%, respectively, compared to uncoated teeth. The mechanism of the Zn:CuO nanoparticles is investigated, revealing that the nanoparticles attach to and penetrate the bacteria and generate intracellular reactive oxygen species (ROS) that enhance lipid peroxidation and cause cell death. Conversely, the CuO or ZnO NPs do not show this behavior and could not generate intracellular ROS. These results highlight the superior efficacy of Zn:CuO nanocomposites over CuO and ZnO NPs and the role of ROS in their antimicrobial effect.

Killing Mechanism of Stable N-Halamine Cross-Linked Polymethacrylamide Nanoparticles That Selectively Target Bacteria

“…This nanocaging was further illustrated via SEM images that show the NPs' 3D morphology (Supporting Information, Figure S7). There are several reports showing that other types of NPs can interact and penetrate cells, 22,24,44,45 but to the best of our knowledge, this is the first documentation of specific and organized interaction of NPs with bacteria, without any traces left in the extracellular environment. These data suggest that chlorinated NPs have a uniquely strong tendency to adhere and circle the bacterial cell wall rather than aggregate.…”

One pot synthesis and anti-biofilm potential of copper nanoparticles (CuNPs) against clinical strains ofPseudomonas aeruginosa

“…AgNP has also been reported to show approximately a 4-6-fold inhibition of P. aeruginosa biofilms (Martinez-Gutierrez et al 2013) whereas Zn:CuO and CuO showed about 88% and 70% inhibition of Streptococcus mutans biofilms, respectively (Eshed et al 2014). The present study reports a maximum of 94% biofilm inhibition against a P. aeruginosa biofilm which is significantly higher than all other available reports.…”

“…CuNPs have also been used as antimicrobial coating agents on medical devices to prevent bacterial contamination (Borkow et al 2010). In spite of their bioactivity, the anti-biofilm potential of CuNPs is seldom explored (Eshed et al 2014). The idea of inhibiting P. aeruginosa biofilm using CuO nanoparticles was raised in the light of the following properties of CuNPs that have already been reported for E. coli and methicillin resistant Staphylococcus aureus (MRSA), and which make CuNP an essential anti-biofilm agent (Agarwala et al 2014): a high potential for (1) absorption, (2) adsorption, (3) penetration and (4) availability.…”

“…The ability of metals to target multiple sites in an organism makes them superior to conventional antibiotics (Sondi & Salopek-Sondi 2004). Extensive work has been done using Zn and Ag nanoparticles against P. aeruginosa biofilms but little work has been done with Cu nanoparticles (CuNPs) (Eshed et al 2014;Palanisamy et al 2014). CuNPs are superconductive, and an easily available and a cost-effective metal known for its wide array of applications.…”

Antibiofilm activity of biogenic copper and zinc oxide nanoparticles-antimicrobials collegiate against multiple drug resistant bacteria: a nanoscale approach

“…Inspite of their bioactivity, the antibiofilm potential of CuNPs is rarely explored [17]. Only a few reports are published in the last year on, the use of copper-containing nanoparticles in the treatment of biofilms [9,18].…”