A β‐subclass phosphatidylinositol‐specific phospholipase C from squid (<i>Loligo forbesi</i>) photoreceptors exhibiting a truncated C‐terminus

Carne, Alan; McGregor, R.; Bhatia, J; Sivaprasadarao, Asipu; Keen, Jeff N.; Davies, Anthony; Findlay, John B. C.

doi:10.1016/0014-5793(95)00936-4

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…Comparison of the deduced amino acid sequence of this PLC with other sequences in the database revealed the protein to be similar in structure and organization to other members of the PLC‐β family of proteins. Highest sequence similarity was found as anticipated to the truncated northern European squid PLC (Carne et al . 1995).…”

Section: Resultsmentioning

confidence: 56%

“…PCR reactions were performed using a squid retinal cDNA library as template (a generous gift from Drs J. K. Northup and M. Brownstein of NIH, Bethesda, MD, USA). A 472 base pair PCR product was generated that showed high sequence similarity to a truncated squid PLC (Carne et al . 1995) and primers based on the sequence from this truncated PLC produced a fragment of approximately 2600 base pairs.…”

Section: Methodsmentioning

confidence: 99%

“…1991), and one from lobster (Xu and McClintock 1999). A cDNA encoding a truncated PLC enzyme was also isolated from squid (Carne et al . 1995).…”

mentioning

confidence: 99%

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Retinal phospholipase C from squid is a regulator of Gqα GTPase activity

Mayeenuddin

Bamsey

Mitchell

2001

Journal of Neurochemistry

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The phospholipase C (PLC) pathway is the major signaling mechanism of photoactivation in invertebrate photoreceptors. Here we report the cloning of a cDNA encoding a 140-kDa retinal PLC that is uniquely expressed in squid photoreceptors. This cDNA encodes a protein with multiple distinct modular domains: PH, X and Y catalytic, and C2 domains, as well as G-and P-box motifs and two GTP/ATP binding motifs. The PLC was stimulated by activated squid Gqa but not by squid Gqbg or mammalian bg subunits. The PLC was inhibited by monophosphate, diphosphate and triphosphate nucleotides but not cyclic nucleosides. We also tested the ability of PLC-140 to regulate the GTPase activity of Gqa in the rhabdomeric membranes. Depletion of PLC-140 from the rhabdomeric membranes decreased the GTP hydrolysis but not GTPgS binding to the membranes. Reconstitution of puri®ed PLC-140 with membranes accelerated Gqa GTPase activity by ®vefold at a concentration of 2.5 mM. Our data suggest that PLC-140 plays an important role in both the activation and inactivation pathways of invertebrate visual transduction.

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Section: Resultsmentioning

confidence: 56%

Section: Methodsmentioning

confidence: 99%

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Retinal phospholipase C from squid is a regulator of Gqα GTPase activity

Mayeenuddin

Bamsey

Mitchell

2001

Journal of Neurochemistry

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“…Three types of PLC are known, PLC-β, PLC-γ and PLC-δ which are regulated by G-proteins, receptor tyrosine kinases, and Ca 2+ , respectively [2]. Animals such as human and rat, but also C. elegans, Artemia, Loligo forbesi and Drosophila possess all three PLC isoforms [3-6]. However, in non-animals exclusively PLC-δ has been identified, e.g.…”

Section: Introductionmentioning

confidence: 99%

Phospholipase Cδ regulates germination of Dictyostelium spores

Dijken

Haastert

2001

BMC Cell Biol

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“…In the case of the squid, this allows not only direct study of the native proteins, but also direct protein sequencing . Using such data, rhodopsin (Hall et al ., 1991), G protein subunits Ryba et al ., 1991 ;Lott et al ., 1992), and a phospholipase Cß (Carne et al ., 1995) have been cloned and characterised within this laboratory .…”

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confidence: 99%

Isolation, Cloning, and Characterisation of a trp Homologue from Squid (Loligo forbesi) Photoreceptor Membranes

Monk

Carne

Liu

et al. 1996

Journal of Neurochemistry

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The invertebrate phototransduction system is a valuable model of the ubiquitous inositol lipid signalling system. Taking advantage of the ability to obtain relatively large amounts of retinal material from the cephalopod eye, partial protein sequence data were obtained for a 92‐kDa component isolated from a detergent‐insensitive cytoskeletal fraction of a squid retinal microvillar membrane preparation. Degenerate oligonucleotides, designed on the basis of these sequence data, were used to isolate a full‐length cDNA, encoding the 92‐kDa component, using both cDNA library screening and 5′‐rapid amplification of cDNA ends (5′‐RACE) techniques. Comparison of the amino acid sequence encoded by this cDNA with entries in the OWL composite protein sequence database reveals greatest sequence similarity with the products of the Drosophila trp and trpl genes. Greatest variation from the Drosophila Trp protein is seen in the carboxyl‐terminal region, which is considerably truncated in the squid protein and which accounts for most of the substantial difference in molecular weight seen between these proteins. This variation may be significant as the carboxyl‐terminal domain has been shown to be in the regulation of several ligand‐gated channels. The carboxyl‐terminal domain has been expressed and shown to interact with calmodulin in a calcium‐dependent fashion, thereby supporting this hypothesis. The likely occurrence of other homologues in a variety of systems suggests that this is a novel and important family of regulated ion channels involved in calcium signalling.

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A β‐subclass phosphatidylinositol‐specific phospholipase C from squid (Loligo forbesi) photoreceptors exhibiting a truncated C‐terminus

Cited by 11 publications

References 39 publications

Retinal phospholipase C from squid is a regulator of Gqα GTPase activity

Retinal phospholipase C from squid is a regulator of Gqα GTPase activity

Phospholipase Cδ regulates germination of Dictyostelium spores

Isolation, Cloning, and Characterisation of a trp Homologue from Squid (Loligo forbesi) Photoreceptor Membranes

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