Aae, an Autotransporter Involved in Adhesion of
            <i>Actinobacillus actinomycetemcomitans</i>
            to Epithelial Cells

Rose, John E.; Meyer, Diane H.; Fives-Taylor, Paula

doi:10.1128/iai.71.5.2384-2393.2003

Cited by 68 publications

(108 citation statements)

References 58 publications

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“…Fresh clinical isolates show a small, wrinkled (rough) colony form Henderson et al, 2003;Kachlany et al, 2001a). The bacteria are known to produce several virulence factors, including a leukotoxin and a cytolethal distending toxin, and they adhere to solid surfaces and cells (Taichman and Wilton, 1981;Kolodrubetz et al, 1989;Lally et al, 1989;Eastcott et al, 1990;Mangan et al, 1991;Fives-Taylor et al, 1999;van Winkelhoff and Slots, 1999;Kachlany et al, 2001a;Narayanan et al, 2002;Henderson et al, 2003;Rose et al, 2003;Paturel et al, 2004;Rhodes et al, 2005;Diaz et al, 2006;Fine et al, 2005Fine et al, , 2006Smith and Bayles, 2006). Clinical isolates autoaggregate and secrete long, bundled pili (Flp fibrils) (Holt et al, 1980;Scannapieco et al, 1987;Rosan et al, 1988;Inoue et al, 1998;Kachlany et al, 2001b;Henderson et al, 2003;Fine et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans

Bhattacharjee

Fine

Figurski

2007

Gene

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AbstracttfoX (sxy) is a regulatory gene needed to turn on competence genes. Aggregatibacter (Actinobacillus) actinomycetemcomitans has a tfoX gene that is important for transformation. We cloned this gene on an IncQ plasmid downstream of the inducible tac promoter. When this plasmid was resident in cells of A. actinomycetemcomitans and tfoX was induced, the cells became competent for transformation. Several strains of A. actinomycetemcomitans, including different serotypes, as well as rough (adherent) and isogenic smooth (nonadherent) forms were tested. Only our two serotype f strains failed to be transformed. With the other strains, we could easily get transformants with extrachromosomal plasmid DNA when closed circular, replicative plasmid carrying an uptake signal sequence (USS) was used. When a replicative plasmid carrying a USS and cloned DNA from the chromosome of A. actinomycetemcomitans was linearized by digestion with a restriction endonuclease or when genomic DNA was used directly, the outcome was allelic exchange. To facilitate allelic exchange, we constructed a suicide plasmid (pMB78) that does not replicate in A. actinomycetemcomitans and carries a region with two inverted copies of a USS. This vector gave allelic exchange in the presence of cloned and induced tfoX easily and without digestion. Using transposon insertions in cloned katA DNA, we found that as little as 78 bp of homology at one of the ends was sufficient for that end to participate in allelic exchange. The cloning and induction of tfoX makes it possible to transform nearly any strain of A. actinomycetemcomitans, and allelic exchange has proven to be important for site-directed mutagenesis.

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Section: Introductionmentioning

confidence: 99%

tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans

Bhattacharjee

Fine

Figurski

2007

Gene

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“…O fenótipo rugoso é fimbriado, autoagregativo, aderente a superficies abióticas, enquanto as variantes lisas são não fimbriadas, podem aderir-se às células epiteliais (ROSE; MEYER; FIVES-TAYLOR, 2003, YUE et al, 2007 e apresentam maior capacidade de invasão que as rugosas (MEYER et al, 1991). Assim, a ausência da transcrição de flp seria esperada nas condições analisadas, visto que as cepas testadas eram lisas, nas quais a ausência da produção da fímbria seria resultado de mutações (TOMICHI et al, 2007).…”

Section: Jp2unclassified

“…Dentre estes fatores, destacam-se mecanismos de adesão e invasão aos tecidos do hospedeiro, liberação de toxinas como a leucotoxina e a toxina distensora citoletal, produção de catalase e proteínas de superfície ligantes ao ferro (WILSON e HENDERSON, 1995;GRABER et al, 1998;MAYER et al, 1999;KOMATSUZAWA et al, 1999;KOMATSUZAWA et al, 2002;MIYAZAKI et al, 1984;NALBANT et al, 2003;ROSE et al, 2003;MINTZ et al, 2004;OUHARA et al, 2006).…”

unclassified

“…O PGA promove adesão intercelular, constitui barreira física e mecânica, e confere à bactéria maior resistência a antibióticos (KAPLAN et al, 2004 actinomycetemcomitans apaH conferiu invasividade a E. coli . Este gene codifica uma diadenosina hidrolase que cliva uma "alarmôna" diadenosina tetrafosfato (Ap4A), uma classe de nucleotídeos sinalizadores do tempo de divisão celular (ROSE et al, 2003;SAARELA et al, 1999). As cepas de A. actinomycetemcomitans podem ser divididas em cinco grupos com relação ao gene apaH (DOGAN et al, 1999), e a maioria das amostras apresenta ApaH contendo a seqüência de aminoácidos RGD que reconhece receptores integrina na superfície de células eucariontes PAJU et al, 1998;PINHEIRO et al, 2010 A. actinomycetemcomitans apresenta também a capacidade de expressar duas toxinas com propriedades citolíticas contra as células humanas, a toxina distensora citoletal e a leucotoxina.…”

unclassified

“…O gene apaH codifica uma diadenosina tetrafosfatase que cliva a "alarmôna" diadenosina tetrafosfato (Ap4A), uma classe de nucleosídeos associada à sinalização do tempo de divisão celular e participação na resposta ao estresse oxidativo (ISMAIL et al, 2003;FIVES-TAYLOR, 2003;SAARELA et al, 1999). A capacidade de metabolizar a diadenosina tetrafosfato poderia ser essencial para a bactéria invadir a célula alvo, sendo que, altas concentrações desta alarmona são encontradas em bactérias mutantes defectivas em gene apaH.…”

unclassified

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Análise da expressão gênica após a interação entre Aggregatibacter actinomycetemcomitans e célula epitelial.

Umeda¹

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Aggregatibacter

Nørskov‐Lauritsen

2021

Bergey's Manual of Systematics of Archaea and Bacteria

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Ag.gre.ga.ti.bac'ter. L. v. aggregare , to come together, aggregate; N.L. masc. n. bacter , rod; N.L. masc. n. Aggregatibacter rod‐shaped bacterium that aggregates with others. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Aggregatibacter The genus Aggregatibacter was established by reclassification of species previously classified in the genera Actinobacillus and Haemophilus . The genus includes typical members of the family Pateurellaceae with respect to cultural characteristics, phenotype, and colony morphology. Humans are the only documented hosts, although Aggregatibacter ‐like bacteria have been cultured from some higher primates. The genus includes four species with validated names. All species of the genus have been cultured from invasive infections of humans. Separation of Aggregatibacter from all other genera of the family Pasteurellaceae by phenotypic characters can be challenging. Partial sequencing of 16S rRNA genes or partial proteomic analysis by mass spectrometry is credible authenticating procedures. Members of the class Gammaproteobacteria , family Pasteurellaceae . Known habitat is the oral cavity of humans. Average DNA G + C content (mol%) : 42.2–44.4. Type species : Aggregatibacter actinomycetemcomitans Nørskov‐Lauritsen and Kilian 2006 VP .

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Aae, an Autotransporter Involved in Adhesion of Actinobacillus actinomycetemcomitans to Epithelial Cells

Cited by 68 publications

References 58 publications

tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans

tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans

Análise da expressão gênica após a interação entre Aggregatibacter actinomycetemcomitans e célula epitelial.

Aggregatibacter

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