ABC transporters in the liver fluke Opisthorchis felineus

Mordvinov, Viatcheslav A.; Ershov, Nikita I.; Pirozhkova, Darya S.; Pakharukov, Yuri V.; Pakharukova, Maria Y.

doi:10.1016/j.molbiopara.2017.07.001

Cited by 14 publications

(11 citation statements)

References 41 publications

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“…Four of ABC genes are homologous to human P-glycoprotein (Additional file 1: Figure S5). Differential expression of these genes was consistent with the earlier data [40].…”

Section: Resultssupporting

confidence: 92%

New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes

et al. 2019

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Background The three epidemiologically important Opisthorchiidae liver flukes Opisthorchis felineus , O. viverrini , and Clonorchis sinensis , are believed to harbour similar potencies to provoke hepatobiliary diseases in their definitive hosts, although their populations have substantially different ecogeographical aspects including habitat, preferred hosts, population structure. Lack of O. felineus genomic data is an obstacle to the development of comparative molecular biological approaches necessary to obtain new knowledge about the biology of Opisthorchiidae trematodes, to identify essential pathways linked to parasite-host interaction, to predict genes that contribute to liver fluke pathogenesis and for the effective prevention and control of the disease. Results Here we present the first draft genome assembly of O. felineus and its gene repertoire accompanied by a comparative analysis with that of O. viverrini and Clonorchis sinensis . We observed both noticeably high heterozygosity of the sequenced individual and substantial genetic diversity in a pooled sample. This indicates that potency of O. felineus population for rapid adaptive response to control and preventive measures of opisthorchiasis is higher than in O. viverrini and C. sinensis . We also have found that all three species are characterized by more intensive involvement of trans-splicing in RNA processing compared to other trematodes. Conclusion All revealed peculiarities of structural organization of genomes are of extreme importance for a proper description of genes and their products in these parasitic species. This should be taken into account both in academic and applied research of epidemiologically important liver flukes. Further comparative genomics studies of liver flukes and non-carcinogenic flatworms allow for generation of well-grounded hypotheses on the mechanisms underlying development of cholangiocarcinoma associated with opisthorchiasis and clonorchiasis as well as species-specific mechanisms of these diseases. Electronic supplementary material The online version of this article (10.1186/s12864-019-5752-8) contains supplementary material, which is available to authorized users.

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“…Four of ABC genes are homologous to human P-glycoprotein (Additional file 1: Figure S5). Differential expression of these genes was consistent with the earlier data [40].…”

Section: Resultssupporting

confidence: 92%

New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes

et al. 2019

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“…These pathways are involved in diverse cellular processes such as maintenance of osmotic homeostasis, nutrient uptake, resistance to xenotoxins, antigen processing, cell division, bacterial immunity, pathogenesis, sporulation, and cholesterol and lipid trafficking. It is also reported that ABC transporters are involved in multidrug resistance in parasites (Jones and George ) and can be prospective targets for study as molecular markers of resistance to antihelminthics through suppression of the transporters themselves with specific inhibitors (Mordvinov, Ershov, Pirozhkova, Pakharukov, & Pakharukova, ). The presented results show that these pathways are important physiological processes, revealing that the function of the microbiome can play an important role in the nutrition and health of their host.…”

Section: Discussionmentioning

confidence: 99%

Composition of the microbial communities in the gastrointestinal tract of perch (Perca fluviatilis L. 1758) and cestodes parasitizing the perch digestive tract

Kashinskaya

Simonov

Извекова

et al. 2019

Journal of Fish Diseases

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Using the approach of sequencing the V3‐V4 region of the 16S rRNA gene, we have analysed the bacterial diversity associated with the distinct compartments of the gastrointestinal tract of perch (Perca fluviatilis) and cestodes (Proteocephalus sp.) parasitizing their digestive tract. The dominant microbiota associated with cestodes (Proteocephalus sp.) was represented by bacteria from the genera Serratia, Pseudomonas and Mycoplasma. By comparing the associated microbiota of perch and cestodes, a clear difference in bacterial composition and diversity was revealed between the community from the stomach content and other parts of the gastrointestinal tract of fish. Microbiota associated with cestodes was not significantly different in comparison with microbiota of different subcompartments of perch (mucosa and content of intestine and pyloric caeca) (ADONIS, p > .05) excluding microbiota of stomach content (ADONIS, p ≤ .05). PICRUSt‐based functional assessments of the microbial communities of perch and cestodes indicated that they mainly linked in terms of metabolism and environmental information processing and could play an important role in the nutrition and health of host.

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“…For collecting OF metacercariae, a naturally infected freshwater fish ( Leuciscus idus ) was net-caught in the Ob River near Novosibirsk (Western Siberia, Russia) by research assistant Viktor Antonov (ICG SB RAS) and was processed without the use of chemicals. OF metacercariae were extracted as described previously ( Mordvinov et al, 2017 ; Pakharukova et al, 2019a ). After several washes with normal saline, metacercariae were identified under a light microscope.…”

Section: Methodsmentioning

confidence: 99%

Global changes in gene expression related to Opisthorchis felineus liver fluke infection reveal temporal heterogeneity of a mammalian host response

Pakharukova

Zaparina

Baginskaya

et al. 2022

Food and Waterborne Parasitology

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ABC transporters in the liver fluke Opisthorchis felineus

Cited by 14 publications

References 41 publications

New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes

New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes

Composition of the microbial communities in the gastrointestinal tract of perch (Perca fluviatilis L. 1758) and cestodes parasitizing the perch digestive tract

Global changes in gene expression related to Opisthorchis felineus liver fluke infection reveal temporal heterogeneity of a mammalian host response

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