Abdominal segment deformity disease (ASDD) of the whiteleg shrimp Penaeus vannamei reared in Thailand

Sakaew, Waraporn; Pratoomthai, Benjamart; Anantasomboon, Gun; Asuvapongpatana, Somluk; Sriurairattana, Siriporn; Withyachumnarnkul, Boonsirm

doi:10.1016/j.aquaculture.2008.07.041

Cited by 18 publications

(13 citation statements)

References 12 publications

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“…As in previous results, semi-thin sections of the abdominal ventral nerve cord revealed an irregular pattern of fibre arrangement [1]. Here, additional anomalies were observed in the cytoplasm of neurons of the abdominal ganglia of ASDD shrimp.…”

Section: Resultssupporting

confidence: 86%

“…Under TEM, numerous viral-like, non-enveloped, icosahedral particles of 20–22 nm diameter were found in the cytoplasm and processes of glial cells, as in a previous report [1]. This study revealed additional abnormal ultrastructure of an axon from ASDD P. vannamei (Figure 8).…”

Section: Resultssupporting

confidence: 86%

“…However, the value of distorted shrimp is approximately 10% less than normal shrimp and farmer losses are proportional to the fraction of a crop that is affected [1]. The impact can be significant, especially when market fluctuations lead to low profit margins.…”

Section: Introductionmentioning

confidence: 99%

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Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei

et al. 2013

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BackgroundAbdominal segment deformity disease (ASDD) of cultivated whiteleg shrimp Penaeus (Litopenaeus) vannamei causes economic loss of approximately 10% in affected specimens because of the unsightliness of distorted abdominal muscles. It is associated with the presence of viral-like particles seen by electron microscopy in the ventral nerve cords of affected shrimp. Thus, shotgun cloning was carried out to seek viral-like sequences in affected shrimp.ResultsA new retrovirus-like element of 5052 bp (named abdominal segment deformity element or ASDE) was compiled by shotgun cloning and 3′ and 5′ RACE using RNA and DNA extracted from ventral nerve cords of ASDD shrimp. ASDE contained 7 putative open reading frames (ORF). One ORF (called the PENS sub-domain), had a deduced amino acid (aa) sequence homologous to the GIY-YIG endonuclease domain of penelope-like retrotransposons while two others were homologous to the reverse transcriptase (RT) and RNaseH domains of the pol gene of non-long terminal repeat (non-LTR) retrotransposons (called the NLRS sub-domain). No single amplicon of 5 kb containing both these elements was obtained by PCR or RT-PCR from ASDD shrimp. Subsequent analysis indicated that PENS and NLRS were not contiguous and that NLRS was a host genetic element. In situ hybridization using a dioxygenin-labeled NLRS probe revealed that NLRS gave positive reactions in abdominal-ganglion neurons of ASDD shrimp but not normal shrimp. Preliminary analysis indicated that long-term use of female broodstock after eyestalk ablation in the hatchery increased the intensity of RT-PCR amplicons for NLRS and also the prevalence of ASDD in mysis 3 offspring of the broodstock. The deformities persist upon further cultivation until shrimp harvest but do not increase in prevalence and do not affect growth or survival.ConclusionsOur results suggested that NLRS is a shrimp genetic element associated with ASDD and that immediate preventative measures could include shorter-term use of broodstock after eyestalk ablation and/or discard of broodstock that give strong RT-PCR reactions for NLRS. In the longer term, it is recommended, if possible, that currently used, domesticated shrimp lines be selected for freedom from NLRS. The molecular tools developed in this work will facilitate the management and further study of ASDD.

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Section: Resultssupporting

confidence: 86%

Section: Resultssupporting

confidence: 86%

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Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei

et al. 2013

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“…The natural host-range of LSNV is presently unknown, but it occurs commonly in Penaeus monodon, and limited studies have suggested a geographical distribution restricted to south and southeast Asia (Sittidilokratna et al 2009). It has also been detected in Litopenaeus vannamei in Thailand affected by abdominal segment deformity disease (Sakaew et al 2008), suggesting that its natural host-range might include species other than P. monodon. There is limited evidence of LSNV occurring in P. monodon cultured in India (Prakasha et al 2007, Rai et al 2009), but it has not been associated with slow growth, and the virus has not been reported from any other crustaceans.…”

Section: Investigations Of Msgs-affected Penaeus Monodon Inmentioning

confidence: 99%

“…HQ728430) possessed 99.0% identity to the Thai LSNV sequence. (Sritunyalucksana et al 2006, Sittidilokratna et al 2009), LSNV has also been detected in Thai Litopenaeus vannamei investigated for abdominal segment deformity disease (Sakaew et al 2008). In India thus far, LSNV has been reported only in P. monodon (Prakasha et al 2007, Rai et al Table 2.…”

Section: Lsnv Sequence Analysismentioning

confidence: 99%

Natural host-range and experimental transmission of Laem-Singh virus (LSNV)

Kumar

Krishnan²,

Makesh³

et al. 2011

Dis. Aquat. Org.

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Slow growth caused by viral diseases has become a major constraint in shrimp aquaculture. Laem-Singh virus (LSNV), a positive-sense single-stranded RNA (ssRNA) virus, has been identified in Penaeus monodon showing slow growth syndrome. To examine the host-range and transmission modes of the virus, 6 species of penaeid shrimp of varying life stages, sourced from the wild and from farms, as well as juvenile mud crabs Scylla serrata, were screened using RT-nested PCR. LSNV was detected in P. monodon, Fenneropenaeus merguiensis, Metapenaeus dobsoni, and Litopenaeus vannamei, but not in F. indicus, Marsupenaeus japonicus or S. serrata. LSNV was most prevalent in P. monodon followed by M. dobsoni, F. merguiensis, and L. vannamei, and real-time quantitative RT-PCR (qRT-PCR) showed that LSNV infection loads were highest in P. monodon, followed by L. vannamei, M. dobsoni, and F. merguiensis. The nucleotide sequence of the LSNV RdRP gene fragment amplified by RT-nested PCR was highly conserved (99% identity) across these 4 penaeid species. LSNV was detected in both small and normal-sized P. monodon collected from the same pond. In experimental infections of both P. monodon and S. serrata, LSNV infection loads increased over time. The present study extends the known natural penaeid host-range and geographical distribution of LSNV and shows for the first time the potential susceptibility of S. serrata.

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Penaeid Prawns

Hai

Fotedar

Hảo

2011

Recent Advances and New Species in Aquaculture

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The study focused on the decline in the Marine fishery resources caught by the trawlers. Different Species of the Marine catch like Skates, Rays, Cat fish, Wolf herring, Anchovies, Stolphorous, Thryssa, OtherClupeids, Flying-fish, other perches, Gastropods and Big Pamfrets declined. The decline in catch rate is more for the species like Halibut followed by Stomatopods and Silver bellies. High valued species like.05) showed a declining trend which affects the economic sustainability of the trawler fishery in Kerala. The most prominent centre of the trawl catch in Kerala namely the Neendakara-Shakthikulangara marked a considerable decline in the varieties of the Marine resources caught as a comparison is made between the years 2002 and 2011.

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Abdominal segment deformity disease (ASDD) of the whiteleg shrimp Penaeus vannamei reared in Thailand

Cited by 18 publications

References 12 publications

Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei

Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei

Natural host-range and experimental transmission of Laem-Singh virus (LSNV)

Penaeid Prawns

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