Aberrant Distribution of Human Adenovirus Type 2 Late Proteins in Monkey Kidney Cells

Cepko, Constance L.; Sharp, Phillip A.

doi:10.1128/jvi.46.1.302-306.1983

Cited by 6 publications

(2 citation statements)

References 26 publications

(18 reference statements)

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“…Several adenovirus mutants which fail to correctly form hexon trimers have been mapped to the gene for the 100K protein (23,29,45,49,53). Cepko and Sharp (10,11) have shown that in abortively infected monkey CV-1 cells, hexon trimer formation is normal. Together with the finding that the 100K protein is not cross-linked to mRNA in abortively infected monkey CV-1 cells, this suggests that the crosslinkable interaction of the 100K protein with mRNA is a manifestation of a function of this protein in a process other than hexon morphogenesis (10,12).…”

Section: Discussionmentioning

confidence: 99%

“…Infection of monkey cells with C-type human adenoviruses results in abortive infection (18,25,26). The 100K protein is produced in CV-1 cells in amounts similar to those produced in human cells, and hexon trimer complexes form as efficiently in these cells as in HeLa cells (11). However, whereas the 100K protein is found predominantly in the cytoplasm of infected HeLa cells late in infection, at comparable times in monkey CV-1 cells it is detected essentially only in the nucleus (11,20,38).…”

Section: Fig 5 Immunoblot Analysis Of Proteins Cross-linked To Polymentioning

confidence: 99%

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Adenovirus proteins associated with mRNA and hnRNA in infected HeLa cells

Adam

Dreyfuss²

1987

J Virol

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The proteins that interact with cytoplasmic and nuclear polyadenylated RNA in adenovirus type 5 (Ad5) infection of HeLa cells were examined by UV-induced RNA-protein cross-linking in intact cells. The Ad5 100-kilodalton late nonvirion protein (1OOK protein) was cross-linked to both host and viral polyadenylated cytoplasmic RNA (mRNA). The cross-linking of the 100K protein to mRNA appears to correlate with productive infection, because the protein is not cross-linked to mRNA in abortive infection of wild-type Ad5 in monkey cells (CV-1) even though normal amounts of it are produced. However, when CV-1 cells are infected with Ad5 hr404, an Ad5 mutant which overcomes the host restriction to wild-type Ad5 infection in these cells, the 100K protein is cross-linked to mRNA. To identify and obtain antibodies to RNA-contacting proteins, a mouse was immunized with oligo(dT)-selected cross-linked RNA-protein complexes from Ad5-infected cells and the serum was used for immunoblotting experiments. It was found that in addition to the 100K protein, the Ad5 72K DNA-binding protein is also associated with RNA in the infected cells. The 72K DNA-binding protein is cross-linked to polyadenylated nuclear RNA sequences. These findings indicate that adenovirus proteins interact with RNAs in the infected cell and suggest possible mechanisms for the effects of the virus on mRNA metabolism.

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