Aberrant expression of CD56 on granulocytes and monocytes in myeloproliferative neoplasm

Gong, Ping; Metrebian, Fernanda; Dulau‐Florea, Alina; Wang, Zixuan; Bajaj, Renu; Gulati, Gene; Peiper, Stephen C.; Gong, Jerald Z.

doi:10.1007/s12308-013-0190-z

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…AA and PNH were characterized by a high proportion of CD56-positive cells in CD34 + myeloid precursors and neutrophils. CD56 is an adhesion molecule that is highly expressed in cells under reactive conditions after chemotherapy, transplantation of hematopoietic stem cells, and active regeneration of BM (Gong et al, 2013). The high expression of CD56 in CD34 + cells, neutrophils, and monocytes should not be overestimated, especially if there are no other features of MDS.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic significance of flow cytometry scales in diagnostics of myelodysplastic syndromes

Davydova

Паровичникова

Galtseva

et al. 2020

Cytometry Part B Clinical

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Background: Myelodysplastic syndromes (MDS) can present a challenge for clinicians. Multicolor flow cytometry (MFC) can aid in establishing a diagnosis. The aim of this study was to determine the optimal MFC approach for MDS. Methods:The study included 102 MDS (39 low-grade MDS), 83 cytopenic patients without myeloid neoplastic disorders (control group), and 35 healthy donors. Bone marrow was analyzed using a six-color MFC. Analysis was conducted according to the "Ogata score," "Wells score," and the integrated flow cytometry (iFC) score. Results:The respective sensitivity and specificity values were 77.5% and 90.4% for the Ogata score, 79.4% and 81.9% for the Wells score, and 87.3% and 87.6% for the iFC score. Specificity was not 100% due to deviations of MFC parameters in the control group. Patients with paroxysmal nocturnal hemoglobinuria (PNH) had higher levels of CD34 + CD7 + myeloid cells than donors. Aplastic anemia and PNH were characterized by a high proportion of CD56 + cells among CD34 + precursors and neutrophils. The proportion of MDS-related features increased with the progression of MDS. The highest number of CD34 + blasts was found in MDS with excess blasts.MDS with isolated del(5q) was characterized by a high proportion of CD34 + CD7 + cells and low granularity of neutrophils. In 39 low-grade MDS, the sensitivities were 53.8%, 61.5%, and 71.8% for Ogata score, Wells score, and iFC, respectively. Conclusion:The results support iFC as a useful diagnostic tool in MDS.

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Section: Discussionmentioning

confidence: 99%