Aberrant expression of lncRNAs and mRNAs in patients with intracranial aneurysm

Wang, Wen; Li, Hao; Yu, Lanbing; Zhao, Zheng; Wang, Haoyuan; Zhang, Dong; Zhang, Yan; Lan, Qing; Wang, Jiangfei; Zhao, Jizong

doi:10.18632/oncotarget.13908

Cited by 25 publications

(32 citation statements)

References 42 publications

(40 reference statements)

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“…MALAT1 has been documented to modulate smooth muscle dysfunction in thoracic aortic aneurysm [8]. Also, a study has presented the abnormal expression of lncRNAs and messenger RNA (mRNA) in IA, and the lncRNA-mRNA co-expression networks provide clues to find the pathogenesis of IA [9]. MALAT1 has been suggested to advance osterix expression and modulates osteogenic differentiation via targeting miRNA-143 (miR-143) expression in human bone marrow mesenchymal stem cells [10].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Long Non-coding RNA MALAT1/microRNA-143/VEGFA Signal Axis Modulates Vascular Endothelial Injury-Induced Intracranial Aneurysm

Gao

Zhang

et al. 2020

Nanoscale Res Lett

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The roles of some long non-coding RNAs (lncRNAs) in intracranial aneurysm (IA) have been investigated in many studies. The aim of this study is to elucidate the mechanism of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/microRNA-143 (miR-143)/vascular endothelial growth factor-A (VEGFA) signal axis in vascular endothelial injury-induced IA. MALAT1, miR-143, and VEGFA expression in IA tissues and normal arterial tissues were detected. Matrix metalloproteinase 9 (MMP-9) in tissues, von Willebrand factor (vWF) in serum and tissues, and endothelin-1 (ET-1) in serum were detected. The modeled IA rats were injected with silenced or overexpressed MALAT1 for detecting vascular endothelial injury. Vascular endothelial cells from patients with IA were abstracted and transfected with silenced or overexpressed MALAT1 to verify the impacts of MALAT1 on cell viability and apoptosis. The connections among MALAT1, miR-143, and VEGFA were verified by online prediction, luciferase activity, and RNA-pull down assays. Overexpression of MALAT1 and VEGFA and poor expression of miR-143 were found in IA tissues. Downregulation of MALAT1 inhibited blood pressure, the expression of ET-1, vWF, and MMP-9, as well as the apoptotic index of vascular endothelial cells of rats with IA. Downregulated MALAT1 inhibited apoptosis and promoted viability of vascular endothelial cells in IA. MALAT1 bound to miR-143 and miR-143 targeted VEGFA. This study suggests that MALAT1 elevates VEGFA expression through competitive binding to miR-143, thereby boosting apoptosis and attenuating viability of vascular endothelial cells in IA.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Long Non-coding RNA MALAT1/microRNA-143/VEGFA Signal Axis Modulates Vascular Endothelial Injury-Induced Intracranial Aneurysm

Gao

Zhang

et al. 2020

Nanoscale Res Lett

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“…Many articles have displayed of the critical roles played by lncRNAs, and it was suggested that lncRNAs are also implicated in cardiovascular diseases, such as aneurysms, myocardial infarction, and atherosclerosis . Furthermore, an lncRNA‐mRNA network was found to regulate inflammatory and immune responses, as well as the contraction of vascular smooth muscle and BSM . In a recent study, some RNA transcripts were shown to act as competing endogenous RNAs (ceRNAs) against miRNAs .…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used a luciferase reporter system to confirm the relationship between miR‐511 and lncRNA‐PAGBC. It was also shown that lncRNA‐PAGBC is implicated in the regulation of PIK3R3 and SOX4 level by binding with miR‐511 and miR‐133b, respectively . Therefore, we used the same reporter system to verify the regulatory relationship between miR‐511 and NOS1.…”

Section: Discussionmentioning

confidence: 99%

“…It was also shown that lncRNA-PAGBC is implicated in the regulation of PIK3R3 and SOX4 level by binding with miR-511 and miR-133b, respectively. 31 Therefore, we used the same reporter system to verify the regulatory relationship between miR-511 and NOS1. In addition to the data obtained from the luciferase assays, we also analyzed the levels of lncRNA-PAGBC, miR-511, NOS1 mRNA, and NO in the A549 and PASMC transfected with lncRNA-PAGBC.…”

Section: Discussionmentioning

confidence: 99%

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Long noncoding RNA PAGBC contributes to nitric oxide (NO) production by sponging miR‐511 in airway hyperresponsiveness upon intubation

2018

J of Cellular Biochemistry

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Background and Objectives In this study, we aimed to study the molecular mechanisms underlying the symptoms of hyperresponsiveness during intubation. Method The value of circulating long noncoding RNA (lncRNA)‐prognosis‐associated gallbladder cancer (PAGBC) in the prediction of hyperresponsiveness upon intubation during general anesthesia was evaluated via the receiver operating characteristic analyses of serum miR‐511, serum PAGBC, and serum nitric oxide (NO). In addition, the possible association between lncRNA‐PAGBC/NOS1 messenger RNA (mRNA) and miR‐511 was further validated via real‐time quantitative polymerase chain reaction, immunohistochemistry assay, computational analysis, and luciferase assay. Enzyme‐linked immunosorbent assay and Western blot analysis were also conducted to establish the regulatory relationship among PAGBC, miR‐511, and NO synthase 1 (NOS1). Results Compared with circulating miR‐511 and serum NO, circulating PAGBC was associated with a higher predictive value. In addition, a negative correlation was found between serum miR‐511 and serum PAGBC (multicorrelation coefficient: −0.5) as well as between serum miR‐511 and serum NO (multicorrelation coefficient: −0.6). In addition, both lncRNA‐PAGBC and NO were decreased in patients with hyperresponsiveness, whereas the levels of miR‐511 and NOS1 in these patients were similar to those in normal patients. Furthermore, our computational analyses and luciferase assays validated the direct binding between miR‐511 and lncRNA‐PAGBC, whereas NOS1 mRNA was identified as a virtual target gene of miR‐511. Moreover, in the presence of lncRNA‐PAGBC, we also observed an evident increase in the levels of NOS1 and NO accompanied by an obvious decrease of miR‐511 expression. Conclusion LncRNA‐PAGBC downregulated the expression of miR‐511, which in turn upregulated the expression of NOS1 mRNA and led to the increase in NOS1 expression, thus leading to the inhibited responsiveness (normal‐responsiveness rather than hyperresponsiveness) to intubation in patients.

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“…The authors further performed lncRNA-mRNA co-expression network analysis (Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG)) and the co-expression networks were represented in immune response, inflammatory response, the muscle contraction pathway, and the vascular smooth muscle contraction pathway. There were 24 lncRNAs that interacted with eight mRNAs in the vascular smooth muscle contraction, 10 lncRNAs interacted with 10 mRNAs in the GO term of immune response, seven lncRNAs interacted with seven mRNAs in the GO term of inflammatory response, and 31 lncRNAs interacted with nine mRNAs in the muscle contraction pathway [27]. ANRIL, also known as CDKN2B-AS, is a long non-coding RNA mostly localized in the nucleus, spanning 126.3 kb in the genome, and its spliced product is a 3834 bp RNA [28].…”

Section: Lncrnas and Iasmentioning

confidence: 99%

The Role of Long Non-Coding RNAs in Intracranial Aneurysms and Subarachnoid Hemorrhage

Gareev

Beylerli

Aliev

et al. 2020

Life

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Intracranial aneurysms (IAs) represent the most complex and relevant problem of modern neurology and neurosurgery. They serve as one of the main causes of non-traumatic subarachnoid hemorrhage (SAH), causing up to 85% of all cases of intracranial hemorrhage, which is associated with frequent disability and high mortality among patients. Unfortunately, the molecular mechanisms of the development and rupture of IAs are still under study. Long non-coding RNAs (lncRNAs) are non-coding RNAs that typically have a length of more than 200 nucleotides. It is known that lncRNAs regulate many processes, such as transcription, translation, cell differentiation, regulation of gene expression, and regulation of the cell cycle. In recent years, a lot of evidence has established their role in human diseases from oncology to cardiovascular disease. Recent studies have shown that lncRNAs may be involved in the pathogenesis of IAs. The study of lncRNAs and its targets in various pathological conditions of a person is a rapidly developing field, and it is likely that the knowledge obtained from these studies regarding the pathogenesis of intracranial aneurysms will have the potential to use lncRNAs in therapy, as well as in the diagnosis and prediction of high aneurysms risk of rupture.

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Aberrant expression of lncRNAs and mRNAs in patients with intracranial aneurysm

Cited by 25 publications

References 42 publications

RETRACTED ARTICLE: Long Non-coding RNA MALAT1/microRNA-143/VEGFA Signal Axis Modulates Vascular Endothelial Injury-Induced Intracranial Aneurysm

RETRACTED ARTICLE: Long Non-coding RNA MALAT1/microRNA-143/VEGFA Signal Axis Modulates Vascular Endothelial Injury-Induced Intracranial Aneurysm

Long noncoding RNA PAGBC contributes to nitric oxide (NO) production by sponging miR‐511 in airway hyperresponsiveness upon intubation

The Role of Long Non-Coding RNAs in Intracranial Aneurysms and Subarachnoid Hemorrhage

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