Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p

Su, Yingying; Ni, Zhaohui; Wang, Guoqing; Cui, Juan; Wei, Chengguo; Wang, Jihan; Yang, Qing; Xu, Ying; Фан, Ли

doi:10.1016/j.intimp.2012.05.016

Cited by 61 publications

(49 citation statements)

References 28 publications

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“…The downregulation of miR-451 was significantly associated with gastric adenocarcinomas as compared with normal gastric mucosa (Bandres et al , 2009; Ueda et al , 2010; Konishi et al , 2012). Similarly, Oh et al (2011) identified miR-486 as a significantly downregulated miRNA in primary GC tumours and GC cell lines, and similar trend was also reported by Konishi et al (2012) and Su et al (2012). Of interest, in the current study, the expression levels of miR-451 and miR-486-5p did not change in culture medium of GC cell lines as cell counts and incubation time increased.…”

Section: Discussionsupporting

confidence: 88%

A five-microRNA panel in plasma was identified as potential biomarker for early detection of gastric cancer

et al. 2014

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Background:Circulating microRNAs (miRNAs) have been implicated as novel biomarkers for gastric cancer (GC) diagnosis. However, the mixture of GC subtypes may have led to the inconsistent circulating miRNA profiles, and the clinical performance of circulating miRNAs has not yet been evaluated independently on early detection of GC.Methods:A four-phase study was designed with a total of 160 cancer-free controls, 124 patients with gastric non-cardia adenocarcinoma (GNCA) and 36 patients diagnosed gastric cardia adenocarcinoma (GCA). In the discovery phase, we screened the miRNA expression profile in plasma of 40 GNCA patients (stage I) and 40 matched controls by TaqMan low density array (TLDA) chips with pooled samples. Differentially expressed miRNAs were further validated in individual sample using quantitative reverse-transcriptase PCR (qRT–PCR) in the training phase. Subsequently, in an independent validation phase, the identified miRNAs were evaluated in 48 GNCA patients (stage I) and 102 matched controls. Finally, the identified miRNAs were further assessed in an external validation phase including advanced GNCA and GCA patients. Additionally, the expression levels of identified miRNAs were measured in the media of BGC823 and MGC803 cell lines.Results:Five miRNAs (miR-16, miR-25, miR-92a, miR-451 and miR-486-5p) showed consistently elevated levels in plasma of the GC patients as compared with controls, and were identified to be potential markers for GNCA with area under the receiver operating characteristic (ROC) curves (AUCs) ranging from 0.850 to 0.925 and 0.694 to 0.790 in the training and validation phases, respectively. The five-miRNA panel presented a high diagnostic accuracy for the early-stage GNCA (AUCs=0.989 and 0.812 for the training and validation phases, respectively). Three miRNAs (miR-16, miR-25 and miR-92a) were excreted into the culture media of GC cell lines.Conclusions:The five-miRNA panel in plasma may serve as a potential non-invasive biomarker in detecting the early-stage GC.

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Section: Discussionsupporting

confidence: 88%

A five-microRNA panel in plasma was identified as potential biomarker for early detection of gastric cancer

et al. 2014

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“…Case-control approach focuses on identifying prognostic markers which are differentially expressed [29, 30]. However, case-only approach interrogates the entire dataset in an unbiased manner [31–33] and may thus yield higher number of prognostic markers. We observed the same in our study, where, with the case–only method, we obtained four and six piRNAs for OS and RFS, respectively as opposed to three piRNAs each for OS and RFS.…”

Section: Discussionmentioning

confidence: 99%

Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer

et al. 2016

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Piwi-interacting RNAs (piRNAs), whose role in germline maintenance has been established, are now also being classified as post-transcriptional regulators of gene expression in somatic cells. PIWI proteins, central to piRNA biogenesis, have been identified as genetic and epigenetic regulators of gene expression. piRNAs/PIWIs have emerged as potential biomarkers for cancer but their relevance to breast cancer has not been comprehensively studied. piRNAs and mRNAs were profiled from normal and breast tumor tissues using next generation sequencing and Agilent platforms, respectively. Gene targets for differentially expressed piRNAs were identified from mRNA expression dataset. piRNAs and PIWI genes were independently assessed for their prognostic significance (outcomes: Overall Survival, OS and Recurrence Free Survival, RFS). We discovered eight piRNAs as novel independent prognostic markers and their association with OS was confirmed in an external dataset (The Cancer Genome Atlas). Further, PIWIL3 and PIWIL4 genes showed prognostic relevance. 306 gene targets exhibited reciprocal relationship with piRNA expression. Cancer cell pathways such as apoptosis and cell signaling were the key Gene Ontology terms associated with the regulated gene targets. Overall, we have captured the entire cascade of events in a dysregulated piRNA pathway and have identified novel markers for breast cancer prognostication.

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“…SMAD4 overexpression could rescue the promoting effects of miR-574-3p on cancer cell growth, which additionally supports the theory that miR-574-3p may be associated with SMAD4. miRNAs are critical regulators and biomarkers for numerous types of cancers (8,(21)(22)(23)(24). There are several miRNAs that have been found to be abnormal in human osteosarcoma, including miR-141, miR-429, miR-451, miR-195, miR-183 miR-199a-3p, miR-127-3p and miR-376c (3,5,10,(25)(26)(27).…”

Section: Downregulation Of Mir-574-3p Inhibited Cell Growth and Inducmentioning

confidence: 99%

miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway

Liu

Zhou

et al. 2016

Oncology Letters

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Abstract. Human osteosarcoma is the most common primary bone malignancy sarcoma that affects primarily children and people <20 years old. In the present study, it was demonstrated that miR-574-3p was downregulated in human osteosarcoma U2OS, SAOS and MG63 cells lines as well as in osteosarcoma tissue compared with the normal tissues. Downregulation of miR-574-3p by antisense miR-574-3p, inhibited cell growth and induced cell apoptosis. Overexpression of miR-574-3p by transfection with miR-574-3p mimics promoted the growth of U2OS cells. The present study then identified mothers against decapentaplegic homolog 4 (SMAD4) as a target of miR-574-3p and SMAD4 was suppressed in miR-574-3p transfected cells. Overexpression of SMAD4 could rescue the promoting effects of miR-574-3p on cancer cell growth. In conclusion, miR-574-3p exerts tumor-promoting roles by targeting the tumor-suppressing gene SMAD4 and its downstream signaling in human osteosarcoma, which provides a novel target for the treatment.

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Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p

Cited by 61 publications

References 28 publications

A five-microRNA panel in plasma was identified as potential biomarker for early detection of gastric cancer

A five-microRNA panel in plasma was identified as potential biomarker for early detection of gastric cancer

Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer

miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway

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