Aberrant Expressional Profiling of Known MicroRNAs in the Liver of Silver Carp (Hypophthalmichthys molitrix) Following Microcystin-LR Exposure Based on samllRNA Sequencing

Abstract

Microcystin-LR (MC-LR) poses a serious threat to human health due to its hepatotoxicity. However, the specific molecular mechanism of miRNAs in MC-LR-induced liver injury has not been determined. The aim of the present study was to determine whether miRNAs are regulated in MC-LR-induced liver toxicity by using high-throughput sequencing. Our research demonstrated that 53 miRNAs and 319 miRNAs were significantly changed after 24 h of treatment with MC-LR (50 and 200 μg/kg, respectively) compared with the control group. GO enrichment analysis revealed that these target genes were related to cellular, metabolic, and single-organism processes. Furthermore, KEGG pathway analysis demonstrated that the target genes of differentially expressed miRNAs in fish liver were primarily involved in the insulin signaling pathway, PPAR signaling pathway, Wnt signaling pathway, and transcriptional misregulation in cancer. Moreover, we hypothesized that 4 miRNAs (miR-16, miR-181a-3p, miR-451, and miR-223) might also participate in MC-LR-induced toxicity in multiple organs of the fish and play regulatory roles according to the qPCR analysis results. Taken together, our results may help to elucidate the biological function of miRNAs in MC-LR-induced toxicity.

Integrated analysis of mRNA and microRNA expression profiles in hepatopancreas of Litopenaeus vannamei under acute exposure to MC-LR

“…Moreover, miR-181a can regulate the immune response by affecting T cells ( Bellon et al, 2009 ; Xie et al, 2013 ). After acute exposure to MC-LR, the expression levels of miR-181a-3p in the liver of juvenile silver carp ( Hypophthalmichthys molitrix ) was significantly decreased in 200 μg/kg groups at 1, 3, and 6 h, upregulated at 24 and 48 h, it may participate in the MC-LR-induced inflammatory response ( Feng et al, 2020 ). After methamphetamine injection, the expression of miR-181a was down-regulated in rat brain, targeting genes involved in protein ubiquitination process and indirectly regulating the down-regulation of GABAAα1 expression ( Wang et al, 2021 ).…”

Female‐to‐male differential transcription patterns of miRNA‐mRNA networks in the livers of dioxin‐exposed mice

“…108 Similarly, the expression of Mir21 was affected in female mouse liver exposed to tumorigenesis-induced microcystins, a class of toxins produced by certain freshwater cyanobacteria. 109,110 Also it was demonstrated that Mir21 and Mir16 were upregulated in cardiac myocytes after treatment with hydrogen peroxide H 2 O 2 and had a protective role in spinal cord against transient ischemia. 111,112 Inversely, Mir21 was repressed by ethanol exposure.…”

Microcystin-LR in Primary Liver Cancers: An Overview

“…An analysis of miRNA expression profiling was conducted in HepG2 cells treated with MC-LR and the expression of has-miR-454-3p, has-miR-449c-5p and has-miR-149-3p was increased in MC-LR-treated cells, while the expression of has-miR-500b-5p, has-miR-500a-5p, has-miR-500a-3p, and has-miR-4286 was decreased [ 64 ]. After exposure to MC-LR, changed miRNA expression was also detected in the liver of juvenile silver carp using small-RNA sequencing, with 372 miRNAs significantly changed after 24 h of treatment with MC-LR [ 65 ]. MiRNA profiling of HL7702 cells exposed to MC-LR was determined via high-throughput sequencing techniques.…”