Aberrant fragment of Dab1 protein is present in yotari mouse

Onoue, Aya; Takeuchi, Mari; Kohno, Takao; Hattori, Mitsuharu

doi:10.1016/j.neures.2014.08.001

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…Antibodies. The antibodies used for Western blotting (WB), immunoprecipitation (IP), immunocytochemistry (ICC), or immunohistochemistry (IHC) were as follows: mouse monoclonal anti-Reelin G10 (MAB5364, Merck Millipore, RRID:AB_2179313; WB 1:2000 and ICC 1:500), mouse monoclonal anti-Reelin E5 (sc-25346, Santa Cruz Biotechnology, RRID:AB_628210; WB 1:1000), rabbit polyclonal anti-GFP (598, MBL, RRID:AB_591819; WB 1:3000, IP 1:1000, IHC 1:1000), rat monoclonal anti-GFP (04404-26, Nacalai Tesque; IHC 1:500), rabbit polyclonal anti-placental alkaline phosphatase (AP, GTX72989, GeneTex, RRID:AB_374630; WB 1:1000), rabbit monoclonal anti-placental AP (ab133602, Abcam; ICC 1:500), rat monoclonal anti-HA 3F10 (11867423001, Roche Diagnostics, RRID:AB_ 390918; WB 50 ng/ml), mouse monoclonal anti-b -actin (013-24553, Wako; WB 1:8000), rat monoclonal anti-Dab1 antibody 4E12 (D355-3, MBL; WB 1:1000) (Onoue et al, 2014), rat monoclonal anti-Dab1 antibody 4H11 (D354-3, MBL; WB 1:1000) (Onoue et al, 2014), mouse monoclonal anti-CTR 12C10 (MABN2428, Merck Millipore, WB 1:500) (Kohno et al, 2015), rabbit monoclonal anti-Nrp1 (ab81321, Abcam, RRID:AB_1640739; WB 1:1000, IHC 1:500), goat anti-VLDLR (AF2258, R&D Systems, RRID:AB_2288612; WB 1:1000, IHC 1:500, IP 1:300), rabbit monoclonal anti-ApoER2 (ab108208, Abcam, RRID:AB_10862841; WB 1:1000), mouse monoclonal antiphosphotyrosine 4G10 (05-321, Merck Millipore, RRID:AB_309678; WB 1:2000), and goat polyclonal TLE (sc-13373, Santa Cruz Biotechnology, RRID:AB_2203721; IP 1:300).…”

Section: Methodsmentioning

confidence: 99%

Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner

et al. 2020

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Reelin plays versatile roles in neocortical development. The C-terminal region (CTR) of Reelin is required for the correct formation of the superficial structure of the neocortex; however, the mechanisms by which this position-specific effect occurs remain largely unknown. In this study, we demonstrate that Reelin with an intact CTR binds to neuropilin-1 (Nrp1), a transmembrane protein.Both male and female mice were used. Nrp1 is localized with very-low-density lipoprotein receptor (VLDLR), a canonical Reelin receptor, in the superficial layers of the developing neocortex. It forms a complex with VLDLR, and this interaction is modulated by the alternative splicing of VLDLR. Reelin with an intact CTR binds more strongly to the VLDLR/Nrp1 complex than to VLDLR alone. Knockdown of Nrp1 in neurons leads to the accumulation of Dab1 protein. Since the degradation of Dab1 is induced by Reelin signaling, it is suggested that Nrp1 augments Reelin signaling. The interaction between Reelin and Nrp1 is required for normal dendritic development in superficial-layer neurons. All of these characteristics of Reelin are abrogated by proteolytic processing of the six C-terminal amino acid residues of Reelin (0.17% of the whole protein). Therefore, Nrp1 is a coreceptor molecule for Reelin and, together with the proteolytic processing of Reelin, can account for context-specific Reelin function in brain development.

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Section: Methodsmentioning

confidence: 99%

Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner

et al. 2020

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“…Anti-Reelin G10 (1:1,000) antibody was purchased from Millipore. Anti-Dab1 monoclonal antibodies (1:1,000) were described previously 60 . Anti-α-tubulin (1:10,000) was purchased from Wako Pure Chemicals (Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Mice that lack the C-terminal region of Reelin exhibit behavioral abnormalities related to neuropsychiatric disorders

Sakai

Shoji

Kohno

et al. 2016

Sci Rep

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The secreted glycoprotein Reelin is believed to play critical roles in the pathogenesis of several neuropsychiatric disorders. The highly basic C-terminal region (CTR) of Reelin is necessary for efficient activation of its downstream signaling, and the brain structure of knock-in mice that lack the CTR (ΔC-KI mice) is impaired. Here, we performed a comprehensive behavioral test battery on ΔC-KI mice, in order to evaluate the effects of partial loss-of-function of Reelin on brain functions. The ΔC-KI mice were hyperactive and exhibited reduced anxiety-like and social behaviors. The working memory in ΔC-KI mice was impaired in a T-maze test. There was little difference in spatial reference memory, depression-like behavior, prepulse inhibition, or fear memory between ΔC-KI and wild-type mice. These results suggest that CTR-dependent Reelin functions are required for some specific normal brain functions and that ΔC-KI mice recapitulate some aspects of neuropsychiatric disorders, such as schizophrenia, bipolar disorder, and autism spectrum disorder.

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“…Poor localization of these two markers suggests their diverse role during renal development (16). In the autosomal recessive mutated yotari mouse, there is a replacement of part of the Dab1 gene with a long-interspersed fragment of the nuclear element (17). Yotari mice express a mutated form of mdab1 mRNA and little or no mDAB1 protein (2).…”

Section: Introductionmentioning

confidence: 99%

The Significance of Expression Pattern of Dab1 and Reelin in the Postnatal Bladder of Dab1 -/- (Yotari) Mice

Čurčić

Racetin

Kelam

et al. 2022

Annals of Biomedical and Clinical Research

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Background. The aim of our study is to provide an insight into the genetic expression landscape of Dab1 and Reelin, which are important in human genitourinary tract development and could help elucidate the critical stages of the onset of bladder anomalies.Methods. Yotari, heterozygote and wild type mice were sacrificed on the 4th, 11th and 14th postnatal day. Morphological parameters were analyzed using immunohistochemistry on mice bladder samples.Results. Significant expression of Dab1 was observed in the bladder epithelium during the 4th postnatal day and in the wt -wild type, but also in the yot -/- mouse. Reelin expression was significant in bladder epithelium during the 4th postnatal day in the wild-type and mutant mouse type. A significant expression of Dab1 positive cells in lamina propria was observed on the 4th postnatal day in yot -/- and in wt. The percentage of Reelin positive cells in lamina propria was significant on the 4th postnatal day in mutated and wild-type mice.Conclusions. An increase in the expression of Dab1 and Reelin proteins in the bladder of a yotari mouse may indicate bladder damage, due to congenital renal abnormalities and caused by a mutation in the Dab1 gene.Key words: Dab1; Reelin, CAKUT, bladder development, yotari mice

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Aberrant fragment of Dab1 protein is present in yotari mouse

Cited by 10 publications

References 17 publications

Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner

Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner

Mice that lack the C-terminal region of Reelin exhibit behavioral abnormalities related to neuropsychiatric disorders

The Significance of Expression Pattern of Dab1 and Reelin in the Postnatal Bladder of Dab1 -/- (Yotari) Mice

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