Aberrant microRNA expression in human cervical carcinomas

Abstract

Because altered microRNAs (miRNAs) expression patterns have been observed in a variety of diseased tissues, miRNA expression was compared in human cervical cancer tissues relative to adjacent normal cervical tissues in the present study. Microarray chips with 924 probes were used to detect the expression of miRNAs in cervical cancer tissue and adjacent normal cervical tissue of 13 patients with cervical cancer (11 squamous cervical cancers, one cervical adenocarcinoma, and one cervical sarcoma), all of whom were infected with human papilloma virus (HPV) 16 and/or HPV18. Compared to the expression levels in normal cervical tissues, 18 miRNAs (1.9%) were significantly upregulated (increase of ≥2×), and 19 miRNAs (2.1%) were significantly downregulated (decrease of ≤0.5×) in cervical cancer tissues. miRNA expression was independent of lymph node involvement, vascular invasion, and pathological differentiation. Taken together, cervical cancer tissues have altered expression of miRNAs relative to adjacent normal tissues. Further studies are necessary to determine whether aberrant miRNA expression is related to the pathogenesis of cervical cancer.

MicroRNA-214 Suppresses Growth and Invasiveness of Cervical Cancer Cells by Targeting UDP-N-acetyl-α-d-galactosamine:Polypeptide N-Acetylgalactosaminyltransferase 7

“…We previously demonstrated that miR-214 was down-regulated in cervical cancer tissue and could negatively regulate HeLa cell growth (14,19). A new microarray analysis also indicated that miR-214 was down-regulated in cervical cancer tissue (20). Additionally, our previous cDNA microarray analysis indicated that GALNT7 increased up to 5.49-fold when miR-214 expression was suppressed in HeLa cells (19).…”

Activities of E6 Protein of Human Papillomavirus 16 Asian Variant on miR-21 Up-regulation and Expression of Human Immune Response Genes

“…However, some studies did not find this to be the case in cervical cancer tissues (Martinez et al, 2008;Li et al, 2011;Rao et al, 2012). Thus, the association of HR-HPV and miR-21 upregulation with cervical cancer development is still limited.…”

The Role of miRNAs in Diagnosis, Prognosis and Treatment Prediction in Cervical Cancer

“…Patients that were enrolled in these studies had not received chemo-or radiotherapy prior to sample collection, which in most cases, occurred during surgery. In this regard, Rao et al [88] determined miRNA profiling of 26 matched cervical cancer and normal tissue samples collected from 13 patients. MiRNA gene expression of cervical cancer tissues and normal adjacent tissues was assessed using CaptialBio mammalian miRNA array V3.0 (CapitalBio, Beijing, China).…”