ABH and related histo‐blood group antigens in normal &amp; malignant human endometrium in relation to genetic and hormonal factors

Skovlund, Vibeke Ravn

doi:10.1111/j.1600-0463.1997.tb05597.x

Cited by 15 publications

(10 citation statements)

References 121 publications

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“…Previous lectin studies have also shown that the binding of UEAI and its glycan epitope, H type antigen, are increased in endometrial carcinoma [51][52][53]. This corroborates with other reports showing that in endometrial carcinoma there is an increased expression of fucosyltransferases responsible for the expression of fucosylated epitopes including H type antigen [54,55]. Also, lectin histochemistry on breast cancer tissues has shown a major reduction of UEAI staining after endo-β-galactosidase digestion, suggesting that UEAI lectin on breast cancer tissues recognizes fucosylated antigens on elongated LacNAc antennae of N-glycans [56].…”

Section: Discussionsupporting

confidence: 90%

Altered glycosylation of glycodelin in endometrial carcinoma

Hautala

Pang

Antonopoulos

et al. 2020

Laboratory Investigation

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Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium. It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype. Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium. We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies. Glycans of HEC-1B Gd were found to comprise a typical mixture of highmannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae. However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found. These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated. This allowed us to select lectins that showed different binding to these classes of glycodelin. Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation. For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin. We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed. In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium. Similar methods can be used for studies of other glycoproteins.

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Section: Discussionsupporting

confidence: 90%

Altered glycosylation of glycodelin in endometrial carcinoma

Hautala

Pang

Antonopoulos

et al. 2020

Laboratory Investigation

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“…Arai (Arai & Nishida, 2003), and Skovlund (1997) showed that LeY is highly expressed in endometrial cancer and related to tumor grade. Theα1,3 fucosylation of LeY is catalyzed by fucosyltransferase IV (FUT4).…”

Section: Fucosyltransferase IV Enhances Expression Of Mmp-12 Stimulatmentioning

confidence: 99%

Fucosyltransferase IV Enhances Expression of MMP-12 Stimulated by EGF via the ERK1/2, p38 and NF-kB Pathways in A431Cells

Yang¹,

Liu²,

Liu³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…Interestingly, estrogen can modulate glycosyltransferase activity in many tissues, 33 and reportedly influences megakaryocytopoiesis and platelet reactivity. [34][35][36][37] When examined by donor sex and age ( Figure 2I), there was a progressive and significant increase in HPA staining on platelets of older female donors (Ͼ50 years; P Ͻ .05), suggesting a relationship between ABH and falling hormone levels during female menopause.…”

Section: Effect Of Donor Age and Sex On Platelet Abh Expressionmentioning

confidence: 99%