Abietanes from Plectranthus grandidentatus and P. hereroensis against methicillin- and vancomycin-resistant bacteria

Gaspar-Marques, Cristina; Rijo, Patrícia; Simões, M. Fátima; Duarte, Aida; Rodrı́guez, Benjamı́n

doi:10.1016/j.phymed.2005.06.002

Cited by 68 publications

(90 citation statements)

References 20 publications

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“…This is in agreement with other studies previously published 7 . Aqueous and methanolic extracts were shown to be inactive against all the microorganisms tested.…”

Section: Discussionsupporting

confidence: 94%

“…tion and characterization of diterpenes, mostly abietanes with a royleanone substructure, showing antimicrobial activities. However, only a few studies have related the diterpene activity with the traditional use of the plant species [6][7] . Some species of the Plectranthus genus have ethnopharmacological applications, with antimicrobial activity often cited 8 .…”

Section: Metodologias De Extracção Do Materials Vegetalmentioning

confidence: 99%

“…[6] [7] Algumas espécies de Plectranthus com aplicações etnofarmacológicas são usadas pela sua acção antimicrobiana. Muitas atividades biológicas têm sido atribuídas a plantas deste género e a sua atividade antimicrobiana é frequentemente citada [8] .…”

Section: Introductionunclassified

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Antimicrobial screening of Plectranthus madagascariensis and P. neochilus extracts

Pereira¹,

Matias²,

Pereira³

et al. 2015

BBR

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127 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Antimicrobial screening of Plectranthus madagascariensis and P. neochilus extracts AbstractNatural products are widely used as traditional medicines and are a common source of bioactive molecules for the treatment of bacterial infections. In particular, some plants of the genus Plectranthus (Lamiaceae) have demonstrated several applications, including the treatment of various infections. In this work, aqueous, acetonic and methanolic extracts of P. madagascariensis and P. neochilus were prepared using several extraction methods (infusion, decoction, maceration, microwave-and ultrasoundassisted and supercritical fluids). All extracts were screened for their antimicrobial activity against Gram positive bacteria (Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis and Mycobacterium smegmatis) and Gram negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli) and two yeast (Candida albicans and Saccharomyces cerevisiae) strains. The P. madagascariensis acetonic extracts obtained using ultrasound and maceration methods and P. neochilus acetonic extract obtained by ultrasound technique showed activity against the five tested Gram positive bacteria (5-24 mm of inhibition zone using the well diffusion test). The antimicrobial activity was further evaluated by the microdilution method (MIC values were between 250-0.49 μg/mL) and the bioautography assay against S. aureus. The P. madagascariensis ultrasound acetonic extract was the most active extract against all the tested Gram positive bacteria (MIC values ranged between 31.25 -0.49 μg/mL). It was also active against resistant MRSA and VRE strains (MIC values ranged between 31.25-0.98 μg/mL). The S. aureus bioautography assay showed that the more polar compounds were the responsible for the antimicrobial activity.Keywords: Antimicrobial activity; natural products; Plectranthus spp; extraction methods. ResumoOs produtos naturais são amplamente utilizados como remédios tradicionais e são uma fonte comum de moléculas bioativas para o tratamento de infeções bacterianas. Em particular, algumas plantas do género Plectranthus (Lamiaceae) demonstraram diversas aplicações, incluindo no tratamento de várias infeções. Neste trabalho, foram preparados extratos aquosos, acetónicos e metanólicos de P. madagascariensis e P. neochilus utilizando vários métodos de extração (infusão, decocção, maceração, extração assistida por micro-ondas e por ultrassom e com fluidos no estado supercrítico). Todos os extratos foram testados quanto à atividade antimicrobiana face a bactérias de Gram-positivo (Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis Bacillus subtilis e Mycobacterium smegmatis) e de Gram-negativo (Pseudomonas aeruginosa, Klebsiella pneumoniae e Escherichia coli) e duas estirpes de leveduras (Candida albicans e Saccharomyces cerevisiae). O extrato acetónico de P. madagascariensis obtid...

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“…This is in agreement with other studies previously published 7 . Aqueous and methanolic extracts were shown to be inactive against all the microorganisms tested.…”

Section: Discussionsupporting

confidence: 94%

Section: Metodologias De Extracção Do Materials Vegetalmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial screening of Plectranthus madagascariensis and P. neochilus extracts

Pereira¹,

Matias²,

Pereira³

et al. 2015

BBR

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“…The resultant was filtered through Whatman paper nº4, vacuum-dried (RV06, IKA, Staufen, Germany) and an aliquot was analyzed using a validated HPLC-DAD method (1200 Infinity, Agilent Technologies, Santa Clara, California, USA), identifying the main components of the extract by comparison to authentic standards 11 .…”

Section: Plant Extract Preparationmentioning

confidence: 99%

“…O resultante foi filtrado em filtro Whatman n.º 4, seco a pressão reduzida (RV06, IKA, Staufen, Alemanha) e uma alíquota foi analisada usando um método validado de HPLC-DAD (1200 Infinity, Agilent Technologies, Santa Clara, Califórnia, EUA) sendo identificados os componentes maioritários do extracto por comparação com amostras autêntitcas 11 .…”

Section: Preparação Do Extracto Vegetalunclassified

Plectranthus madagascariensis phytosomes: formulation optimization

Matias¹,

Roque²,

Simões³

et al. 2015

BBR

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Biopharmaceutical Sciences│Ciências BiofarmacêuticasBiomed Biopharm Res. , 2015; (12) 2: , 223-231 223 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Plectranthus madagascariensis phytosomes: formulation optimization AbstractMedicinal plants have been a reliable source of bioactive natural products with potential pharmaceutical applications. The delivery of those bioactive agents into nanosystems is considered a promising strategy for the optimization of their pharmacologic effects. This work describes the preparation of phytosomes containing a bioactive extract from Plectranthus madagascariensis and optimization of the preparation method. Different formulations and process parameters were studied. It was observed that smaller and more uniform particles were obtained using acetone as solvent, a reaction time of two hours, and the addition of 2.5% molar concentration of cholesterol. The optimally prepared phytosomes had a diameter of 191.3 ± 75.3 nm with a polydispersity index of 0.243 ± 0.18, and a spherical shape with amorphous appearance. These nanosystems were able to encapsulate 92.8% of the extract, as evaluated by HPLC, relative to 7α,6β-dihydroxyroyleanona, the main extract component. This study suggests a future application of those phytosomes in the delivery of bioactive agents with therapeutic interest.Keywords: Phytosome, Plectranthus, Lamiaceae, Diterpenes, Formulation optimization ResumoAs plantas medicinais são uma fonte interessante de produtos naturais bioactivos com potenciais aplicações farmacêuticas. A veiculação destes agentes bioactivos em nanossistemas é considerada uma estratégia promissora para a optimização dos seus efeitos farmacológicos. O objectivo deste trabalho incide na preparação de fitossomas contendo um extracto bioactivo de Plectranthus madagascariensis e a sua optimização no método de preparação. Foram estudados vários parâmetros de formulação e processo. Observou-se que partículas menores e mais uniformes foram obtidas utilizando acetona como solvente, um período de reacção de 2h e com a adição do colesterol a uma concentração molar de 2,5%. Os fitossomas apresentaram um diâmetro de 191,3 ± 75,3 nm e com um índice de polidispersividade de 0,243 ± 0,18. Os fitossomas apresentaram uma forma esférica mas com aspecto amorfo. Estes nanossistemas foram capazes de encapsular 92,8% do extracto avaliado por HPLC em relação ao composto 7α,6β-dihidroxi-roileanona, o principal composto do extracto. Este estudo sugere uma futura aplicação destes fitossomas na veiculação de agentes bioactivos com interesse terapêutico.

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Natural Diterpene and Triterpene Quinone Methides: Structures, Synthesis, and Biological Potentials

Zhou

2009

Quinone Methides

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Abietanes from Plectranthus grandidentatus and P. hereroensis against methicillin- and vancomycin-resistant bacteria

Cited by 68 publications

References 20 publications

Antimicrobial screening of Plectranthus madagascariensis and P. neochilus extracts

Antimicrobial screening of Plectranthus madagascariensis and P. neochilus extracts

Plectranthus madagascariensis phytosomes: formulation optimization

Natural Diterpene and Triterpene Quinone Methides: Structures, Synthesis, and Biological Potentials

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