Ability of Bacillus to Degrade Geosmin

Danglot, C.; Amar, G; Vilagines, R.

doi:10.2166/wst.1983.0153

Cited by 13 publications

(3 citation statements)

References 3 publications

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“…(1987) could not reproduce these results using the same strains. In addition, Danglot et al. (1983) reported the unsuccessful attempts to degrade geosmin using spent culture filtrates of B. subtilis .…”

Section: Introductionmentioning

confidence: 99%

“…As then there have been conflicting reports, with Narayan and Nunez (1974) reporting that strains of B. cereus and B. subtilis isolated by soil-enrichment culture readily degraded geosmin; however, MacDonald et al (1987) could not reproduce these results using the same strains. In addition, Danglot et al (1983) reported the unsuccessful attempts to degrade geosmin using spent culture filtrates of B. subtilis. As then there has only been one other report of bacterial degradation of geosmin (Saadoun and El-Migdadi 1998), where an alcohol transformation test was used to demonstrate that cultured strains of various gram-positive bacteria, including Arthrobacter atrocyaneus, Arthrobacter globiformis, Chlorophenolicus strain N-1053 and Rhodococcus maris, were capable of degrading this compound in axenic culture with geosmin as the sole carbon source.…”

Section: Introductionmentioning

confidence: 99%

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Cooperative biodegradation of geosmin by a consortium comprising three gram-negative bacteria isolated from the biofilm of a sand filter column

Hoefel

Aunkofer

et al. 2006

Lett Appl Microbiol

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Aims: To isolate and identify bacteria from a sand filter column capable of degrading the taste and odour compound, geosmin. In doing so, to investigate if these organisms degrade geosmin either individually or if an alternative mechanism is utilized. Methods and Results: Geosmin‐degrading bacteria from a biologically active sand filter column were enriched by their growth in a minimal medium supplemented with geosmin as the sole carbon source. By day 51, 21·7 mg l−1 of geosmin had been degraded as determined by solid‐phase microextraction gas chromatography/mass spectrometry, and was accompanied by a 2·12 log10 increase in active bacterial numbers as measured using the BacLightTM bacterial viability kit and flow cytometric enumeration. During the onset of geosmin degradation, the predominance of three bacteria, most similar to previously cultured species of Sphingopyxis alaskensis, Novosphingobium stygiae and Pseudomonas veronii based on 16S rRNA gene sequences was detected by denaturing gradient gel electrophoresis. Subsequent isolation of these organisms revealed that degradation of geosmin, when present as either the sole carbon source (ranging from 40 ng l−1 to 20 mg l−1) or when spiked into sterile reservoir water (37 and 131 ng l−1), occurred only when all three isolates were present. None of the isolates was shown to be capable of degrading geosmin either individually or in any combination of two. Conclusions: This study has reported, for the first time, the cooperative degradation of geosmin by a consortium comprising three gram‐negative bacteria isolated from a biologically active sand filter column. Significance and Impact of the Study: These results are important for researchers currently employing molecular‐based approaches to further understand the biodegradation of geosmin by bacteria, as such studies may be complicated by the discovery of geosmin degradation occurring by a consortium. This study also advances the knowledge surrounding the types of bacteria capable of degrading the taste and odour compound, as investigations to date regarding this are limited.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cooperative biodegradation of geosmin by a consortium comprising three gram-negative bacteria isolated from the biofilm of a sand filter column

Hoefel

Aunkofer

et al. 2006

Lett Appl Microbiol

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“…Lack of study at realistic odour compound concentrations and disregard for losses of these compounds by other means such as volatilization or adsorption, include some of the limitations of previous studies. Danglot et al (1983) reported the physical adsorption of geosmin onto glass vessels or peristaltic pump tubing. The study of these compounds at ng/L levels has magnified the problems created by systemic losses of odour compounds.…”

Section: Introductionmentioning

confidence: 99%

Determination of System Losses of Geosmin and MIB in Bench-Scale Filtration Apparatus

Elhadi

Huck

Slawson

2004

Water Quality Research Journal

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Geosmin and 2-methylisoborneol (MIB) are two of the causative compounds responsible for the earthy/musty odour problem in drinking water. They are a major concern for the water treatment industry because they are difficult to remove by conventional water treatment practices and are fairly resistant to chemical oxidation. Various studies have been conducted at both bench and full scale to examine the removal of these compounds by adsorption and/or biological filtration. Lack of study at realistic odour compound concentrations and disregard for losses of these compounds by other means (i.e., volatilization or adsorption) represent some of the limitations of such studies. The bench-scale investigation reported herein focussed on system design and minimization of system losses. The results indicated that the preferred system design included the use of Teflon feed bottles, Teflon tubing, and a glass filter column in which the influent feed line descended close to the media surface. Such a design minimized system losses of both geosmin and MIB to 42 and 30%, respectively, based on target influent concentrations of 200 ng/L.

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