Ability of human plasma to protect against ionising radiation is inversely correlated with age

Lenton, K J; Greenstock, C.L.

doi:10.1016/s0047-6374(98)00128-6

Cited by 13 publications

(10 citation statements)

References 13 publications

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“…Ageing is usually followed by disturbances in micronutritient status and accumulated point muations of repair genes: folate and vitamin B 12 concentrations are particularly important determinants of micronucleus frequency (28,29). Recent studies on men aged 50-70 years have confirmed these observations (30,31).…”

Section: Discussionmentioning

confidence: 95%

Age-related changes in radiation-induced micronuclei among healthy adults

Joksić

Petrović

Ilić

2004

Braz J Med Biol Res

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The aim of the present study was to establish the extent of in vitro radioresponse of lymphocytes among 62 healthy adults of both genders and to estimate the distribution of baseline micronuclei and radiosensitivity among individuals of the study population using the cytochalasin block micronucleus test. A younger study group consisted of 10 males (mean age, 22.4 years; range, 21-27) and 12 females (mean age, 24.8 years; range, 20-29), whereas an older study group consisted of 18 males (mean age, 35.1 years; range, 30-44) and 22 females (mean age, 38.5 years; range, 30-48). For evaluation of radiosensitivity blood samples were irradiated in vitro using 60 Co γ-ray source. The radiation dose employed was 2 Gy, the dose rate 0.45 Gy/ min. The study revealed a significant gender effect on baseline micronuclei favoring females (Z = 3.25, P < 0.001), while yields of radiation-induced micronuclei did not differ significantly (Z = 0.56, P < 0.56) between genders. The distribution of baseline micronuclei among the individuals tested followed Poisson distribution in both study groups and in both genders, whereas the distribution of radiosensitivity among individuals of the older study group did not fulfill Poisson expectations (Kolmogorov-Smirnof test, P < 0.01). In contrast to a nonsignificant difference in radiosensitivity between males and females of the same age group (Z = 1.97, P < 0.56), a statistically significant difference in radiosensitivity between younger and older group for both genders was found (Z = 3.03, P < 0.03). Since the individuals tested were healthy, the observed variability in radiation response is considered to be an early effect of ageing.

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Section: Discussionmentioning

confidence: 95%

Age-related changes in radiation-induced micronuclei among healthy adults

Joksić

Petrović

Ilić

2004

Braz J Med Biol Res

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“…Reactive oxygen species (ROS) including superoxide anion radical (O 2 − ), hydroxyl radical ( • OH) and hydrogen peroxide (H 2 O 2 ) are generated during normal aerobic metabolism (1) and are increased under the stress of ionizing radiation and chemical reagents. ROS are considered to cause oxidative damage of biological macromolecules such as DNA, lipids and proteins, leading to cancer, senescence and other diseases (2)(3)(4)(5). To combat these effects of ROS, living organisms have developed an antioxidant defence system that includes scavenging proteins and compounds to transform ROS into harmless molecules, e.g.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA in Deinococcus radiodurans

Teng

Sheng

et al. 2004

Luminescence

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Free radical scavenging effects of the cellular protein extracts from two strains of Deinococcus radiodurans and Escherichia coli against O2-, H2O2 and *OH were investigated by chemiluminescence (CL) methods. The cellular protein extracts of D. radiodurans R1 and KD8301 showed higher scavenging effects on O2- than that of E. coli. D. radiodurans R1 and KD8301 also strongly scavenged H2O2 with an EC50 (50% effective concentration) of 0.12 and 0.2 mg/mL, respectively, compared to that of E. coli (EC50 = 3.56 mg/mL). The two strains of D. radiodurans were effective in scavenging *OH generated by the Fenton reaction, with EC50 of 0.059 and 0.1 mg/mL, respectively, compared to that of E. coli (EC50 > 1 mg/mL). Results from the chemiluminescence assay of *OH-induced DNA damage and the plasmid pUC18 DNA double-strand break (DSB) model in vitro showed that D. radiodurans had remarkably inhibitory effect on the *OH-induced oxidative damage of DNA. The scavenging effects of D. radiodurans on reactive oxygen species (ROS) played an important role in the response to oxidation stress and preventing against DNA oxidative damage, and may be attributed to intracellular scavenging proteins, including superoxide dismutase (SOD) and catalase.

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“…Investigation on humans aged 30–80 years reported inverse relationship between plasma radioprotective ability to in vitro 26 Gy γ -irradiation and donor age, based on total plasma AO capacity [16]. Study on gerbils observed that AO potential of tissue homogenates from older animals had a reduced ability to protect against radiation-induced oxidative damage provoked by 60 Gy X-irradiation, measured by protein oxidation susceptibility.…”

Section: Discussionmentioning

confidence: 99%

“…However, an age-related modulation of AO system and AO enzyme expression in radiation response in humans is poorly investigated. The only obtainable report was given by Lenton and Greenstock [16], who showed inverse relationship between human plasma radioprotective ability to 26 Gy γ -irradiation and donor age, based on total plasma AO capacity. Thus, in this work we investigated the AO enzyme activities of copper, zink superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), as well as concentration of glutathione (GSH) in blood cells of healthy women after in vitroγ -irradiation of blood.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging

Kasapović

Stojiljković

Gavrilović

et al. 2012

The Scientific World Journal

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Reactive oxygen species (ROS) are independently recognized to play a significant role in radiation-induced damage on healthy tissue and in aging process. However, an age-related alteration of antioxidant (AO) system in radiation response in humans is poorly investigated. The aim of this paper was to evaluate the irradiation effects on the activities and expression of AO system in the blood of healthy women during aging. Blood samples were irradiated with curative and palliative doses of 2 Gy or 9 Gyγ-rays. AO capacity for detoxification of O2•−and H2O2in response to 2 Gyγ-irradiation decreases in women above 58 years, while in response to 9 Gy shows signs of weakening after 45 years of age. Due to reduction of AO capacity during aging, cytotoxic effects of curative and palliative doses of irradiation, mediated by ROS, may significantly increase in older subjects, while removal of H2O2excess could reduce them.

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Ability of human plasma to protect against ionising radiation is inversely correlated with age

Cited by 13 publications

References 13 publications

Age-related changes in radiation-induced micronuclei among healthy adults

Age-related changes in radiation-induced micronuclei among healthy adults

Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA in Deinococcus radiodurans

Antioxidant Protection against Curative and Palliative Doses of Ionizing Irradiation in Human Blood Decreases with Aging

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