Ability of indigenous Bacillus licheniformis and Bacillus subtilis in microbial enhanced oil recovery

Abstract

ABSTARCT: Microbially produced lipopeptide have been isolated and studied for microbial enhanced oil recovery.About 60 gram positive bacteria isolated from soil contaminated with crude oil, near the crude oil storage tank in Tehran Refinery, Tehran, Iran. However, most of these studies have produced lipopeptide by one of the pure-culture microbes isolated in a laboratory. Among the isolates, heamolytic tests revealed two biosurfactant producers. The isolated strains were designated as C2, E1. By using morphological, biochemical and molecular biology tests (16 SrRNA), the strains identified as Bacillus licheniformis and Bacillus subtitlis, respectively. Emulsification activity and measurement of surface tension indicated that, the isolates were high producers of biosurfactant. The product of C2 and E1 is mainly lipopeptide. This product reduce surface tension from 65 to 30 mN/m. Emulsified activity of crude oil was 92% for C2 and 90 % in case of E1. This is the first report of indigenous Bacillus licheniformis and Bacillus subtilis from a soil contaminated with oil in an Iranian refinery with ability to produce biosurfactant.

Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment

“…The fate of PAHs in the environment is associated with both abiotic and biotic processes, including volatilization, photooxidation, chemical oxidation, bioaccumulation and microbial transformation. Microbial activity has been deemed the most influential and significant cause of PAH removal (Cerniglia, 1993;Nwuche and Ugoji, 2008;Haghighat et al, 2008;Agbozu and Opuene, 2009;Atlas and Bragg, 2009). Considerable attention has focused on th e metabolic path ways and genetics of degradation of low molecular mass PAHs, such as naphthalene, phenanthrene and anthracene, by gramnegative bacteria (Williams and Sayers, 1994).…”

Enhancement of Crude Oil Biodegradation by Immobilizing of different Bacterial Strains on Porous PVA Hydrogels or Combining of them with their produced Biosurfactants

“…A crosslinked prepared PVA (CPVA) carrier was chosen for comparison and it was used for the preparation of the immobilization matrix. The main advantages in the use of immobilized cells in comparison with suspended ones include the retention in the reactor of higher concentration of microorganisms, easy removal of bacteria after use from the reaction mixture, providing the ability to control reaction time, reuse of cells for many reaction cycles, lowering the total production cost of cells-mediated reactions, provide pure products [24][25][26]. The efficiencies of each of free, immobilized cells (Bacillus licheniformis, Rhodococcuserythropolishas and Pseudomonas xanthomarina) on CPVAF and CPVA matrixes crosslinked by epichlorohydrin [17], and their biosurfactants in degradation of crude oil in water were evaluated.…”

Identification of potential local isolated for biosurfactant production

“…Isolates were screened on blood agar plates containing 5% (v/v) sheep blood and incubated at 37°C for 48 h. Hemolytic activity was detected as the presence of a clear zone around bacterial colonies [7,8,18].…”