Abiotic Stress Phenotyping of Polyamine Mutants

Berberich, Thomas; Sagor, G. H. M.; Kusano, Tomonobu

doi:10.1007/978-1-4939-7398-9_32

Cited by 3 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To obtain total conductivity, samples were incubated at 121°C for 15 min, allowed to cool to room temperature, and then the electrical conductivity (C2) measured. The EL was calculated using the formula: % EL = (C1/C2) × 100%, as previously described (Berberich et al ., 2018).…”

Section: Methodsmentioning

confidence: 99%

Molecular basis for neofunctionalization of duplicated E3 ubiquitin ligases underlying adaptation to drought tolerance inArabidopsis thaliana

et al. 2020

View full text Add to dashboard Cite

Multigene families ina plants expanded from ancestral genes via gene duplication mechanisms constitute a significant fraction of the coding genome. Although most duplicated genes are lost over time, many are retained in the genome. Clusters of tandemly arrayed genes are commonly found in the plant genome where they can promote expansion of gene families. In the present study, promoter fusion to the GUS reporter gene was used to examine the promoter architecture of duplicated E3 ligase genes that are part of group C in the Arabidopsis thaliana ATL family. Acquisition of gene expression by AtATL78, possibly generated from defective AtATL81 expression, is described. AtATL78 expression was purportedly enhanced by insertion of a TATA box within the core promoter region after a short tandem duplication that occurred during evolution of Brassicaceae lineages. This gene is associated with an adaptation to drought tolerance of A. thaliana. These findings also suggest duplicated genes could serve as a reservoir of tacit genetic information, and expression of these duplicated genes is activated upon acquisition of core promoter sequences. Remarkably, drought transcriptome profiling in response to rehydration suggests that ATL78-dependent gene expression predominantly affects genes with root-specific activities.

show abstract

Section: Methodsmentioning

confidence: 99%

Molecular basis for neofunctionalization of duplicated E3 ubiquitin ligases underlying adaptation to drought tolerance inArabidopsis thaliana

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The levels of superoxide O 2 - were determined based on its ability to reduce nitro blue tetrazolium (NBT) to blue formazan as previously described [59,60]. Dry seeds (0.035g) were grinded in 1.3 mL of incubation solution (10 mM K-phosphate buffer pH 7.8, 10 mM NaN 3 , 0.05% NBT).…”

Section: Methodsmentioning

confidence: 99%

polyamine uptake transporter 2 (put2) and decaying seeds enhance phyA-mediated germination by overcoming PIF1 repression of germination

et al. 2019

View full text Add to dashboard Cite

Red light promotes germination after activating phytochrome phyB, which destabilizes the germination repressor PIF1. Early upon seed imbibition, canopy light, unfavorable for photosynthesis, represses germination by stabilizing PIF1 after inactivating phyB. Paradoxically, later upon imbibition, canopy light stimulates germination after activating phytochrome phyA. phyA-mediated germination is poorly understood and, intriguingly, is inefficient, compared to phyB-mediated germination, raising the question of its physiological significance. A genetic screen identified polyamine uptake transporter 2 ( put2 ) mutants that overaccumulate polyamines, a class of antioxidant polycations implicated in numerous cellular functions, which we found promote phyA-mediated germination. In WT seeds, our data suggest that canopy light represses polyamines accumulation through PIF1 while red light promotes polyamines accumulation. We show that canopy light also downregulates PIF1 levels, through phyA; however, PIF1 reaccumulates rapidly, which limits phyA-mediated germination. High polyamines levels in decaying seeds bypass PIF1 repression of germination and stimulate phyA-mediated germination, suggesting an adaptive mechanism promoting survival when viability is compromised.

show abstract

SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana

Yan

Zhang

et al. 2022

Sci. China Life Sci.

View full text Add to dashboard Cite

Abiotic Stress Phenotyping of Polyamine Mutants

Cited by 3 publications

References 29 publications

Molecular basis for neofunctionalization of duplicated E3 ubiquitin ligases underlying adaptation to drought tolerance inArabidopsis thaliana

Molecular basis for neofunctionalization of duplicated E3 ubiquitin ligases underlying adaptation to drought tolerance inArabidopsis thaliana

polyamine uptake transporter 2 (put2) and decaying seeds enhance phyA-mediated germination by overcoming PIF1 repression of germination

SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana

Contact Info

Product

Resources

About