Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

Брагина, Е. Е.; Arifulin, Eugene A.; Лазарева, Е. М.; Лелекова, М. А.; Коломиец, О. Л.; Чоговадзе, А. Г.; Сорокина, Т. М.; Курило, Л. Ф.; Поляков, Array В.

doi:10.17650/2070-9781-2017-18-1-48-61

Cited by 5 publications

(1 citation statement)

References 91 publications

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“…The overall appearance of spermatozoa was studied at a magnification of x4000, acrosomes, nuclear chromatin, and mitochondria at x25000, and axonemal anomalies on cross-sections of flagella at x25000. In each sample, at least 150 gametes were analyzed [15].…”

Section: Methodsmentioning

confidence: 99%

Potentials of electron microscopic examination in the diagnosis of genetically determined disorders related to morphology and motility of human spermatozoa

Bragina,

Zadubenko,

Kalimagambetov

et al. 2024

BIO Web Conf.

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Despite infertility is known as a serious medical and social issue, commercial panels for the determination of genetically caused disorders of morphology and motility of spermatozoa are not available in the territory of the Eurasian Economic Union. Electron microscopic examination of spermatozoa (EMIS) represents a promising method that allows to visualize and analyze structural anomalies in spermatozoa at a level which is not accessible by other methods. The study included transmission electron microscopy (TEM) of spermatozoa. Native sperm was diluted and fixed with 2.5% glutaraldehyde. Ultrathin sections were obtained on an UltraCut III microtome. Analysis was performed at magnifications of x4000, x25000. Spermatozoa were analyzed on a JEM-1011 electron microscope at different magnifications to identify the general appearance, axonemal anomalies, chromatin of the nucleus, and mitochondria. EMIS serves as a tool for detailed analysis of the morphology of spermatozoa in patients with asthenozoospermia (AZS) and teratozoospermia (TZS), and their combination. The obtained data provide a basis for more accurate diagnosis and personalized treatment approaches, contributing to increased effectiveness in overcoming infertility.

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Section: Methodsmentioning

confidence: 99%

Potentials of electron microscopic examination in the diagnosis of genetically determined disorders related to morphology and motility of human spermatozoa

Bragina,

Zadubenko,

Kalimagambetov

et al. 2024

BIO Web Conf.

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Subfertility in a married couple: emphases on pathogenesis and correction

Posiseeva¹,

Петрова²

2018

Ross. vestn. akush.-ginekol.

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Фертильность человека-это его способность к размножению в период половозрелости (с 15 до 49 лет). Фертильность женщины и мужчины является интегральным показателем здоровья человека. Факторы, способные негативно повлиять на фертильность, весьма многообразны и относятся к различным категориям. Снижение фертильности в настоящее время распространено довольно широко, причем как среди женщин, так и среди мужчин. Клиническими проявлениями субфертильности могут служить как бесплодие, так и нереализованные в полноценное деторождение зачатия в виде спонтанных абортов, внематочной беременности, мертворождений. Выявление вероятных причин снижения фертильности в супружеской паре, их коррекция являются актуальной задачей здравоохранения, особенно в настоящее время в связи с решением демографических проблем в стране. Причинами женской субфертильности могут служить гипоталамо

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Long-Term Results of Treatment of Patients With Chronic Nonbacterial Prostatitis and Increased Level of Sperm Dna Fragmentation With the Prostatilen® Ac Drug

Боровец¹,

Rybalov²,

Горбачев³

et al. 2018

Androl. genit. hir.

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The study objectiveis to evaluate the results of treatment of patients with chronic nonbacterial prostatitis, increased level of sperm DNA fragmentation, and concomitant reproductive function disorders with the Prostatilen® AC drug 1, 2, and 3 months after the end of therapy.Materials and methods. Thirty five men aged 21–46 years (mean age 31.3 ± 4.3 years) with a verified diagnosis of “chronic nonbacterial prostatitis, associated reproductive function disorders, history of early-stage missed miscarriage in a wife/sex partner” were examined. All patients received Prostatilen® AC as rectal suppositories daily for 10 days with a repeat course 20 days later. Before and after the treatment, as well as after 1, 2, and 3 months after its end, the fraction of spermatozoa with fragmented DNA was measured in all patients using sperm chromatin structure assay with flow cytofluorometry. The risk of decreased fertility was considered low if the number of spermatozoa with fragmented DNA was 15 % or lower (this value is considered the norm). If DNA fragmentation was 16 % or higher, it was considered increased.Results. The fraction of spermatozoa with fragmented DNA significantly decreased during the treatment, and 1 month after the end of the therapy it was 7.5 ± 4.2 % (prior to treatment it was 17.8 ± 6.7 %) (p <0.005). Prior to Prostatilen® AC treatment, enhanced DNA fragmentation was observed in 18 (51 %) patients, 1 month after treatment – only in 6 (17 %); the difference is statistically significant (p <0.001). Two months after the end of treatment, increased level of DNA fragmentation was detected in 7 (20 %) patients, 3 months after – in 15 (42 %) patients. Aside from that, if the fraction of spermatozoa with fragmented DNA was more than 30 % before treatment, then a decrease in this value during treatment wasn’t statistically significant.Conclusion. Prostatilen® AC promotes a reduction in the level of sperm DNA fragmentation in patients with chronic nonbacterial prostatitis. A steady positive effect persists for 2 months with a tendency to weaken at the end of month 3 which indicates advisability of repeated courses of Prostatilen® AC treatment 2 months after the end of therapy.

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Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

Cited by 5 publications

References 91 publications

Potentials of electron microscopic examination in the diagnosis of genetically determined disorders related to morphology and motility of human spermatozoa

Potentials of electron microscopic examination in the diagnosis of genetically determined disorders related to morphology and motility of human spermatozoa

Subfertility in a married couple: emphases on pathogenesis and correction

Long-Term Results of Treatment of Patients With Chronic Nonbacterial Prostatitis and Increased Level of Sperm Dna Fragmentation With the Prostatilen® Ac Drug

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