Abnormal levels of seven amino neurotransmitters in depressed rat brain and determination by HPLC‐FLD

Cui, Ting; Qiu, Hongmei; Huang, Dan; Zhou, Qing; Fu, Xiaoyan; Li, Haiyan; Jiang, Xinhui

doi:10.1002/bmc.3937

Cited by 17 publications

(7 citation statements)

References 32 publications

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“…The effect of reaction time (1, 2, 5, 10, 20, 30, and 60 min after vortexing for 30 s) on the peak area of AA derivatives was tested using the established HPLC‐FLR method (Figure 6). The derivatization condition was developed based on the method suggested by Cui et al (2017). The results showed that the peak area of most AAs changed greatly in the first 10 min of reaction time, but no interference peak was observed during 60 min of reaction time.…”

Section: Resultsmentioning

confidence: 99%

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The variation in the levels of 18 amino acids in the cortex and plasma of cerebral ischemia C57BL/6 mice

Qiu

Jiang

et al. 2021

Biomedical Chromatography

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Emerging evidence suggests that amino acid (AA) neurotransmitters play important roles in the pathophysiological processes of cerebral ischemia. In this work, an HPLC with fluorescence detection (HPLC‐FLR) method was developed for the simultaneous determination of 18 AAs in the cortex and plasma after cerebral ischemia in mice. The ischemia model was prepared by bilateral common carotid artery occlusion, and then the cortex and plasma of the sham, ischemia, and naringenin groups were collected. Based on the protein precipitation method, a simple and effective sample preparation method was developed. The treated sample contained minimal proteins and lipids. The analysis of the sample was performed by the proposed HPLC‐FLR method in combination with o‐phthalaldehyde. The results showed a statistically significant increase in excitatory AAs (aspartic acid and glutamic acid), inhibitory AAs (glycine and 4‐aminobutyric acid), phenylalanine, citrulline, isoleucine, and leucine levels, and a decrease of glutathione and phenylalanine levels when compared with the sham group in the cortex. Besides, the administration of naringenin had significant effects on excitatory AAs, inhibitory AA (glycine), glutamine, tyrosine, phenylalanine, and leucine levels when compared with the sham group in the cortex. These findings could be utilized in studying and clarifying the mechanisms of ischemia.

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Section: Resultsmentioning

confidence: 99%

“…The pre‐column derivatization procedure was performed by modifying the method reported by Cui et al (2017). Briefly, 16.1 mg OPA was dissolved in 0.4 mL methanol, then 3.57 mL borate buffer and 0.03 mL β‐mercaptoethanol were added in the aforesaid mixture (prepared weekly and stored at 4°C).…”

Section: Methodsmentioning

confidence: 99%

The variation in the levels of 18 amino acids in the cortex and plasma of cerebral ischemia C57BL/6 mice

Qiu

Jiang

et al. 2021

Biomedical Chromatography

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“…The new scheme was obtained by modifying the OPA derivatization method reported by Cui Ting (Cui et al, ). The preparation of derivatization reagent was as follows: to 400 μl methanol containing 16.1 mg of OPA was added 3.57 ml 0.1 mol·L −1 borax buffer (pH 10.0) and 30 μl β ‐mercaptoethanol, newly prepared weekly and stored at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous determination of thirteen substances related to NAFLD in mouse brain tissue using 3‐aminobutyric acid as internal standard by HPLC–FLD

Liu

Wan

et al. 2020

Biomedical Chromatography

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Disorders of certain branched‐chain amino acids may be associated with the occurrence and development of non‐alcoholic fatty liver disease. Measurement of related branched‐chain amino acid levels could provide a reference for the clinical and scientific research of the non‐alcoholic fatty liver disease. An established HPLC–FLD method was used to quantify aspartic acid, glutamate, glutamine, glycine, taurine, tyrosine, 4‐amino butanoic acid, tryptophan, methionine, valine, phenylalanine, isoleucine and leucine in mouse brain tissue. Brain tissue samples mixed with internal standard (3‐aminobutyric acid) were processed, then derivatized with 2‐O‐phthaldialdehyde, and finally separated on an ODS2 column through gradient elution at a flow rate of 1.0 ml·min−1. The excitation and emission wavelengths were set at 340 and 455 nm, respectively. The mobile phase A was 100% methanol and the mobile phase B consisted of 30 mmol·L−1 sodium acetate (pH 6.8). The injection volume was 20 μl and the single run time was 45 min. Several parameters, accuracy, precision, and stability, were verified and the results showed the established method had good sensitivity and resolution for all of the 13 compounds and internal standard in mouse brain.

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“…The glutamate level in the hippocampus was determined by high performance liquid chromatography (HPLC; Agilent 1260, Thermo Fisher Scientific, Waltham, MA, USA), according to the protocol described by Cui et al (2017) (19). The hippocampus sample was added to homogenization buffer and centrifuged to obtain supernatant, which was suspended with O-phthaldialdehyde.…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Herba Rhodiolae alleviates depression via the BDNF/TrkB-GSK-3β signaling pathway

Gao

Liao

et al. 2021

Ann Transl Med

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Background: Depression is one of the most common psychiatric disorders worldwide. Many antidepressant drugs are only effective for specific patients and their long-term use can lead to treatment resistance. There is an ongoing need for more effective antidepressant treatments. Methods:The rats were exposed to CUMS to induce depression model. The glutamate level in the hippocampus was determined by HPLC. Protein levels in the tissues were measured using western blotting.The fluid consumption test was performed to evaluate potential anhedonia. Open field test was conducted to evaluate locomotor activity. Forced swimming test was used to evaluate susceptibility to negative mood.Results: In the present study, the depressive-like behaviors induced by chronic unpredictable mild stress (CUMS) were alleviated in rats by treatments with Herba Rhodiolae (0.04 g/kg, 1 mL), venlafaxine (0.035 g/kg, 1 mL), and combination of Herba Rhodiolae and glycogen synthase kinase 3 beta (GSK-3β) inhibitor (10 μM, 1 mL). Among these treatments, the combination of Herba Rhodiolae and GSK-3β inhibitor was most effective. Abnormalities in the glutamate system and autophagy of the brain were decreased greatly in Herba Rhodiolae and GSK-3β inhibitor-treated CUMS rats. Herba Rhodiolae was more effective in promoting the brain derived neurotrophic factor/tropomyosin receptor kinase B (BDNF/ TrkB) signaling. The expression of GSK-3β was remarkably suppressed by the GSK-3β inhibitor.Conclusions: These findings indicated that the combined effects of Herba Rhodiolae and GSK-3β inhibitor contributed to the activation of BDNF/TrkB-GSK-3β signaling pathway.

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Abnormal levels of seven amino neurotransmitters in depressed rat brain and determination by HPLC‐FLD

Cited by 17 publications

References 32 publications

The variation in the levels of 18 amino acids in the cortex and plasma of cerebral ischemia C57BL/6 mice

The variation in the levels of 18 amino acids in the cortex and plasma of cerebral ischemia C57BL/6 mice

Simultaneous determination of thirteen substances related to NAFLD in mouse brain tissue using 3‐aminobutyric acid as internal standard by HPLC–FLD

Herba Rhodiolae alleviates depression via the BDNF/TrkB-GSK-3β signaling pathway

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