Abnormal Pancreatic Isoamylases in the Serum of Children with Choledochal Cyst

Abstract

Abnormal pancreatic isoamylases, especially P4 and P5, are found in the serum of patients with choledochal cyst, which would point to a reflux of pancreatic juice into the biliary tract.

Activated Pancreatic Enzyme and Pancreatic Stone Protein (PSP/reg) in Bile of Patients with Pancreaticobiliary Maljunction/Choledochal Cysts

“…Many previous studies of pancreaticobiliary maljunction demonstrated high levels of amylase and/or lipase in the bile as evidence of regurgitation of pancreatic juice into the bile duct (19)(20)(21)(22)(23)(24)(25)(26)(27). However, only five studies have documented activation of proenzymes in the bile under pancreaticobiliary maljunction (6)(7)(8)(9)(10).…”

Potential diagnostic value of pancreatic isoamylases for pancreaticobiliary maljunction with mild biliary dilatation in patients and a porcine model

“…This knowledge has prompted us to look for abnormal pancreatic isoamylases in patients with CC. 7,8 With the same technique in the current study, we attempted to evaluate the diagnostic value of isoamylases for this rare subset of patients.…”

“…7,8,10 Isoamylases were separated in a standard electrophoresis cell on nitrocellulose membranes (Amersham, Piscataway, NJ) with a discontinuous buffer system. The anodal chamber contained TRIS base at 0.15 m/L titrated to a pH of 9.15 with boric acid.…”

“…In the normal pattern, there were 3 peaks of P2, P1, and S, or 2 peaks of P2 and S. Other peaks after P2 were interpreted as abnormal P3, P4, P5, and P6. Therefore, the sequence of appearance of the peaks from the cathode toward the anode was P6-P1, S. 8 Peak appearance rate (PAR) of each P peak was calculated as follows: number of cases showing the peak/total number of cases in this group ϫ 100%. Precise 2 was used for statistic analysis.…”

“…In the bile and serum of children with CC, we found abnormal pancreatic isoamylases, especially P4 and P5, which differed from those of normal controls and the patients with acute pancreatitis. 7,8 In the current study, we extended our earlier work to evaluate the diagnostic value of serum pancreatic isoamylases for PBM with mild biliary dilatation.…”