Abnormalities on 1q and 7q are associated with poor outcome in sporadic Burkitt's lymphoma. A cytogenetic and comparative genomic hybridization study

Abstract

Comparative genomic hybridization (CGH) studies have demonstrated a high incidence of chromosomal imbalances in nonHodgkin's lymphoma. However, the information on the genomic imbalances in Burkitt's Lymphoma (BL) is scanty. Conventional cytogenetics was performed in 34 cases, and long-distance PCR for t(8;14) was performed in 18 cases. A total of 170 changes were present with a median of four changes per case (range 1-22). Gains of chromosomal material (143) were more frequent than amplifications (5) or losses (22). The most frequent aberrations were gains on chromosomes 12q (26%), Xq (22%), 22q (20%), 20q (17%) and 9q (15%). Losses predominantly involved chromosomes 13q (17%) and 4q (9%). High-level amplifications were present in the regions 1q23-31 (three cases), 6p12-p25 and 8p22-p23. Upon comparing BL vs Burkitt's cell leukemia (BCL), the latter had more changes (mean 4.372.2) than BL (mean 2.773.2). In addition, BCL cases showed more frequently gains on 8q, 9q, 14q, 20q, and 20q, 9q, 8q and 14q, as well as losses on 13q and 4q. Concerning outcome, the presence of abnormalities on 1q (ascertained either by cytogenetics or by CGH), and imbalances on 7q (P ¼ 0.01) were associated with a short survival.

A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility

“…We reported previously on regional chr.7q21-q22 over-representations in close association with progression of HCC to advance metastatic tumors (Poon et al, 2006). Recurrent proximal gains of chr.7q are in fact common in human neoplasms (Knuutila et al, 1998), where it has been implicated in increased metastatic potentials of colorectal cancer (Nakao et al, 2001), angiogenesis in prostate tumors (Strohmeyer et al, 2004) and inferior survival of patients with sporadic Burkitt's lymphoma (Garcı´a et al, 2003). We identified PFTK1 as target proto-oncogene within the aberrant genomic amplicon chr.…”

Secondary chromosomal abnormalities predict outcome in pediatric and adult high‐stage Burkitt lymphoma

“…These abnormalities have been documented in approximately one-third of BL cases by others. 3,4,9,47,48 Chromosome 1 abnormalities also occur in approximately one-third of follicular lymphomas, 31 of germinal center cell origin similar to BL, where they appear to have a negative impact on prognosis. 42 The biological significance of these abnormalities is unclear.…”

Translocations involving 8q24 in Burkitt lymphoma and other malignant lymphomas: a historical review of cytogenetics in the light of todays knowledge

“…The lack of complex genetic alterations in addition to a MYC translocation might therefore be suggestive for a 'true' BL. 28,29 The presence of an additional translocation as found in other types of B-NHL (for example, involving BCL2, BCL6 and/or CCND1) or a complex karyotype with multiple gains and/or losses would rather represent another type of B-NHL or a secondary transformed lymphoma. 30 This is supported by the publication of the MMML where array based comparative genomic hybridization (arrayCGH) was used to study genomic copy number imbalances in addition to fluorescent in situ hybridization (FISH) analysis for MYC, BCL2 and BCL6 translocations.…”