2009
DOI: 10.1080/09674845.2009.11730251
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ABO reverse grouping: effect of varying concentrations of the enzyme bromelain

Abstract: Proteolytic enzymes can be extremely useful for the identification of clinically significant antibodies in blood grouping; however, they can also be destructive to certain agglutination reactions. ABO antibodies are the most clinically significant. A review of the literature reveals little research involving proteolytic enzymes and IgM antibodies. This study investigates the effects of various concentrations of the proteolytic enzyme bromelain on the agglutination of reagent A1 and B cells with donor plasma du… Show more

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Cited by 6 publications
(5 citation statements)
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“…Currently, there exist several automated and semiautomated work stations with available comparative studies based on different technologies, such as column agglutination, solid‐phase, microplate, and red blood cell (RBC)‐magnetized methods. Although all these devices and methods have significantly contributed to the improvement testing performance, none has been unequivocally established as superior to others.…”
supporting
confidence: 91%
“…Currently, there exist several automated and semiautomated work stations with available comparative studies based on different technologies, such as column agglutination, solid‐phase, microplate, and red blood cell (RBC)‐magnetized methods. Although all these devices and methods have significantly contributed to the improvement testing performance, none has been unequivocally established as superior to others.…”
supporting
confidence: 91%
“…Elasticity and ζ potential can be somehow connected by some common factor? RBCs glycoproteins are sensitive to enzymes and can be even removed by them [8]. During RBCs storage period, the leukocyte degranulation promotes enzyme release, which can reduce glycoprotein membrane expressions and probably can change the membrane electrical charges [9].…”
Section: Introductionmentioning
confidence: 99%
“…Bromelain has been investigated to be applied in clinically important antibody identification (Rookard et al 2009). Papain and ficin are used for the preparation of Fab fragments from IgG to be employed in assay systems where the presence of the Fc region may cause problems (Mariani et al 1991;Boguslawski et al 1989).…”
Section: Use Of Cysteine Proteases In Pharmacy and Medicinementioning
confidence: 99%