Abortos por Neosporacaninum em bovinos do sul de Minas Gerais

Orlando, Débora Ribeiro; Costa, R. A.; Soares, Bruno Alvarenga; Oliveira, Natália Souza; Nascimento, Lismara Castro do; Peconick, Ana Paula; Raymundo, Djeison Lutier; Varaschin, Mary Suzan

doi:10.1590/s0100-736x2013001100008

Cited by 16 publications

(16 citation statements)

References 31 publications

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“…Corbellini et al (2000) found 10% of the fetuses to be positive when they analyzed tissues of 30 fetuses using the immunohistochemical technique (IHC). Orlando et al (2013), after analyzing tissues of 60 fetuses through PCR and IHC, found 23.33% of the samples to be positive for the protozoan. Meanwhile, analyzing the brain, lung, liver, heart and intrathoracic fluid of 30 fetuses of dairy cattle from the State of Santa Catarina, Macedo et al (2017) found 38.8% positives through the PCR technique, 25% through immunohistochemistry and 26.7% through of the ELISA.…”

Section: Aborted Bovine Fetusmentioning

confidence: 99%

Neospora caninum in properties in the west region of Paraná, Brazil: prevalence and risk factors

Snak

Garcia

Lara

et al. 2018

Rev. Bras. Parasitol. Vet.

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Neospora caninum is a heteroxenous protozoa, whose definitive hosts are canids and intermediate hosts are herbivores, and is of great importance in cattle. The objectives of this study were to determine the prevalence of N. caninum in dairy cattle and dogs, to detect the presence of the protozoa at the molecular level in aborted fetuses, and to identify the risk factors associated with infection in properties in the western region of the state of Paraná. For this study, 600 bovine serum samples from 60 properties, 163 canine serum samples from 52 properties and 17 bovine fetuses from nine properties were collected. Data were collected using an epidemiological questionnaire to verify the risk factors. Serum samples were analyzed using the indirect fluorescent antibody test. Fetal tissues were analyzed using polymerase chain reaction and subsequent DNA sequencing. Of the bovine samples, 23.67% were positive for N. caninum. Among the canine samples, 11.66% were positive for N. caninum. Risk factors in cattle were history of abortion, low milk production, extensive breeding, and Jersey breed (p<0.05). Protozoan DNA was detected in 52.94% of the 17 fetuses and the sequencing presented high similarity with N. caninum.

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Section: Aborted Bovine Fetusmentioning

confidence: 99%

Neospora caninum in properties in the west region of Paraná, Brazil: prevalence and risk factors

Snak

Garcia

Lara

et al. 2018

Rev. Bras. Parasitol. Vet.

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“…In Brazil, N. caninum was considered an important abortifacient agent in cattle (CORBELLINI et al, 2002;SANTOS et al, 2005b;CORBELLINI et al, 2006;ORLANDO et al, 2013;PESCADOR et al, 2007;CABRAL et al, 2009). Corbellini et al (2002) showed in Rio Grande do Sul state, southern Brazil, 81% of the aborted fetuses with brain lesions suggestive of N. caninum.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.Keywords: Neosporosis, fetuses, lesion, serology, cattle. ResumoO objetivo deste estudo foi avaliar a ocorrência de N. caninum associado a abortamentos em vacas de leite do estado de Santa Catarina, sul do Brasil pelo uso das técnicas de ELISA (HerdCheck, IDEXX), reação em cadeia pela polimerase (PCR) e imunohistoquímica (IHC). O sangue das vacas leiteiras que abortaram, bem como, o líquido intratorácico e amostras de tecidos (cérebro, coração, fígado e pulmão) de seus fetos foram coletados e usados para sorologia, PCR (Np21+ e Np6+), e IHC. Vinte e uma vacas (51,2%) de um total de 41, bem como, oito fetos (26,7%) de um total de 30 foram positivos no ELISA (IDEXX) para N. caninum. As vacas > 36 meses de idade tiveram um maior risco de serem soropositivas do que os animais mais jovens. PCR e IHC revelaram que 38,8% e 25,0% dos fetos foram positivos para N. caninum, respectivamente para cada um dos testes. As vacas soropositivas tiveram uma maior frequência de fetos que também foram positivos no fluído intratorácico, na PCR ou na IHC. Em resumo, o presente estudo observou uma alta frequência de N. caninum em abortos de vacas leiteiras na região estudada, com maior prevalência de N. caninum em vacas com mais de 36 meses de idade. Além disso, sorologia, PCR e IHC deveriam ser utilizadas conjuntamente para melhor diagnóstico de neosporose em bovinos.

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“…Dezenove fetos, que apresentaram lesões compatíveis com infecção por N. caninum e confirmação diagnóstica por IHQ e PCR em 14 deles, foram descritos por Orlando et al (2013). Nos outros 30 fetos, sem lesões microscópicas, não podem ser descartadas causas não infecciosas de aborto, evidenciadas no histórico desses casos; em duas propriedades houve relato de medicação com corticoide nas vacas gestantes e, em várias propriedades, havia histórico de escassez alimentar.…”

Section: Discussionunclassified

“…Entre as diversas etiologias do aborto, destacam-se as de origem infecciosa, com abortos causados por protozoários, bactérias, vírus e fungos. Abortos por protozoários em bovinos são associados principalmente à infecção por Neospora caninum (Corbellini et al 2002, Orlando et al 2013, mas podem ser causados também por Tritrichomonas foetus (Alves et al 2011). Abortos bacterianos são associados a Brucela abortus, Leptospira sp., Listeria monocytogenes e Campylobacter sp.…”

Section: Introductionunclassified

Caracterização morfológica e imuno-histoquímica de lesões em casos de aborto bovino bacteriano e viral no sul de Minas Gerais

et al. 2014

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Lavras, from 1999Lavras, from to 2013. From 60 fetuses studied, 30 (50%) had microscopic lesions. From these, eight had lesions consistent with bacterial agents and three had lesions suggestive of viral agents. In the bacterial abortions, one fetus presented lesions compatible with leptospirosis, characterized by jaundice, cholestasis, lymphoplasmacytic intersticial nephritis, and tubular nephrosis. Seven fetuses had purulent pneumonia or bronchopneumonia and one of them had also fibrinous pleuritis and peritonitis; two of them presented positive immunostaining for Brucella abortus. One of the three fetuses with lesions of viral infection revealed positive imunostaining for bovine herpesvirus. The results showed that abortions of bacterial and viral origin occur in the Region of this study and prophylactic measures should be adopted on the farms. This study also demonstrates that immunohistochemistry associated with histopathology is a useful and viable tool for the diagnosis, especially when microbiological and/or serological tests are not available.

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Abortos por Neosporacaninum em bovinos do sul de Minas Gerais

Cited by 16 publications

References 31 publications

Neospora caninum in properties in the west region of Paraná, Brazil: prevalence and risk factors

Neospora caninum in properties in the west region of Paraná, Brazil: prevalence and risk factors

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Caracterização morfológica e imuno-histoquímica de lesões em casos de aborto bovino bacteriano e viral no sul de Minas Gerais

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