Abrupt emergence and predominance in Vietnam of rotavirus A strains possessing a bovine-like G8 on a DS-1-like background

Hoa-Tran, Thi Nguyen; Nakagomi, Toyoko; Vu, Hung Manh; P., L.; Gauchan, Punita; Agbemabiese, Chantal Ama; Nguyen, Thi-Hoai-Thu; Nakagomi, Osamu; Thanh, Nguyen Thi Hien

doi:10.1007/s00705-015-2682-x

Cited by 51 publications

(70 citation statements)

References 12 publications

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“…For example, a high frequency of G8P [6] has been reported in São Tom e and Pr ıncipe in Africa [Istrate et al, 2015]. Recently, DS-1-like G8P[8] strains similar to those in Thailand detected in this study were found in a high proportion (27%) in Vietnam in 2015 [Hoa-Tran et al, 2016].…”

Section: Discussionsupporting

confidence: 59%

Predominant prevalence of human rotaviruses with the G1P[8] and G8P[8] genotypes with a short RNA profile in 2013 and 2014 in Sukhothai and Phetchaboon provinces, Thailand

Guntapong

Tacharoenmuang

Singchai

et al. 2016

Journal of Medical Virology

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Of 2,754 stool specimens collected from children with acute gastroenteritis during 2013-2014 in Sukhothai and Phetchaboon provinces, Thailand, 666 (24.2%) were positive for rotavirus A (RVA) in polyacrylamide gel electrophoresis (PAGE). The G and P types of all RVA-positive specimens were determined by semi-nested RT-PCR. G1P[8] (56.5%) was most prevalent, followed by G2P[4] (22.1%). Unusual G8P[8] human RVAs (HuRVAs) were detected at a high frequency (20.0%). Interestingly, 171 of the 376 G1P[8] HuRVAs and all of the 133 G8P[8] HuRVAs showed a short RNA pattern in PAGE. Thus, it was shown that the properties of HuRVAs have been markedly unusual in recent years in Thailand. J. Med. Virol. 89:615-620, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 59%

Predominant prevalence of human rotaviruses with the G1P[8] and G8P[8] genotypes with a short RNA profile in 2013 and 2014 in Sukhothai and Phetchaboon provinces, Thailand

Guntapong

Tacharoenmuang

Singchai

et al. 2016

Journal of Medical Virology

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“…The genomes of these G8P[8] strains shared >99.6% nt identity with each other (Table 2; Technical Appendix). All 11 genome segments of strain To14-0 (the representative G8P[8] strain in this study) exhibited the highest nucleotide identity to human G8P[8] strains isolated in Southeast Asia in 2014 (represented by strain RVN1149 from Vietnam [>99.4% nt identity] and NP-130 from Thailand [>99.5% nt identity]) ( 6 , 7 ) (Table 2). This finding suggests that the strains share a common G8P[8] origin.…”

Section: The Studymentioning

confidence: 93%

Clinical and Molecular Characteristics of Human Rotavirus G8P[8] Outbreak Strain, Japan, 2014

Kondo¹,

Tsugawa²,

Ono³

et al. 2017

Emerg. Infect. Dis.

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During March–July 2014, rotavirus G8P[8] emerged as the predominant cause of rotavirus gastroenteritis among children in Hokkaido Prefecture, Japan. Clinical characteristics were similar for infections caused by G8 and non-G8 strains. Sequence and phylogenetic analyses suggest the strains were generated by multiple reassortment events between DS-1–like P[8] strains and bovine strains from Asia.

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“…Furthermore, in 2013–2014, we identified DS-1-like intergenogroup reassortant strains having G8P[8] genotypes (including strains KKL-17, PCB-79, PCB-84, PCB-85, PCB-103, SKT-107, SWL-12, NP-130, PCB-656, SKT-457, SSKT-269, and SSL-55) in Thailand as major strains [29]. Similar DS-1-like G8P[8] strains (including strain RVN1149) were subsequently detected in Vietnam in 2014–2015 [25]. G8, like G6 and G10, is one of the common G genotypes in cattle that has been identified in humans sporadically [30].…”

Section: Introductionmentioning

confidence: 99%

“…G8, like G6 and G10, is one of the common G genotypes in cattle that has been identified in humans sporadically [30]. Although human G8 strains are particularly prevalent in Africa, they have been infrequently detected elsewhere [25, 31]. In 2015, the emergence of DS-1-like G3P[8] strains (including strains ERN8263 and SS96217158) was recognized in Hungary and Spain, Europe [21, 23].…”

Section: Introductionmentioning

confidence: 99%

“…To date, however, the full genome sequences of only two DS-1-like intergenogroup reassortant strains having G8P[8] genotypes, strain RVN1149 from Vietnam and strain DRC88 from the Democratic Republic of Congo, have been sequenced and characterized, indicating the presence of bovine-like segments on a DS-1-like genetic backbone [25, 32]. However, because the overall genomic constellation and genomic diversity of DS-1-like G8P[8] strains remain to be elucidated, full genomic analysis of Thai DS-1-like G8P[8] strains might be useful for obtaining a more precise understanding of the evolutionary dynamics of the emerging DS-1-like intergenogroup reassortant strains involving DS-1-like G8P[8] HuRVAs.…”

Section: Introductionmentioning

confidence: 99%

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Full Genome Characterization of Novel DS-1-Like G8P[8] Rotavirus Strains that Have Emerged in Thailand: Reassortment of Bovine and Human Rotavirus Gene Segments in Emerging DS-1-Like Intergenogroup Reassortant Strains

et al. 2016

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The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotavirus strains have been recently reported in Asia, Australia, and Europe. During rotavirus surveillance in Thailand in 2013–2014, novel DS-1-like intergenogroup reassortant strains having G8P[8] genotypes (i.e., strains KKL-17, PCB-79, PCB-84, PCB-85, PCB-103, SKT-107, SWL-12, NP-130, PCB-656, SKT-457, SSKT-269, and SSL-55) were identified in stool samples from hospitalized children with severe diarrhea. In this study, we determined and characterized the complete genomes of these 12 strains (seven strains, KKL-17, PCB-79, PCB-84, PCB-85, PCB-103, SKT-107, and SWL-12, found in 2013 (2013 strains), and five, NP-130, PCB-656, SKT-457, SSKT-269, and SSL-55, in 2014 (2014 strains)). On full genomic analysis, all 12 strains showed a unique genotype constellation comprising a mixture of genogroup 1 and 2 genes: G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. With the exception of the G genotype, the unique genotype constellation of the 12 strains (P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2) was found to be shared with DS-1-like intergenogroup reassortant strains. On phylogenetic analysis, six of the 11 genes of the 2013 strains (VP4, VP2, VP3, NSP1, NSP3, and NSP5) appeared to have originated from DS-1-like intergenogroup reassortant strains, while the remaining four (VP7, VP6, VP1, and NSP2) and one (NSP4) gene appeared to be of bovine and human origin, respectively. Thus, the 2013 strains appeared to be reassortant strains as to DS-1-like intergenogroup reassortant, bovine, bovine-like human, and/or human rotaviruses. On the other hand, five of the 11 genes of the 2014 strains (VP4, VP2, VP3, NSP1, and NSP3) appeared to have originated from DS-1-like intergenogroup reassortant strains, while three (VP7, VP1, and NSP2) and one (NSP4) were assumed to be of bovine and human origin, respectively. Notably, the remaining two genes, VP6 and NSP5, of the 2014 strains appeared to have originated from locally circulating DS-1-like G2P[4] human rotaviruses. Thus, the 2014 strains were assumed to be multiple reassortment strains as to DS-1-like intergenogroup reassortant, bovine, bovine-like human, human, and/or locally circulating DS-1-like G2P[4] human rotaviruses. Overall, the great genomic diversity among the DS-1-like intergenogroup reassortant strains seemed to have been generated through additional reassortment events involving animal and human strains. Moreover, all the 11 genes of three of the 2014 strains, NP-130, PCB-656, and SSL-55, were very closely related to those of Vietnamese DS-1-like G8P[8] strains that emerged in 2014–2015, indicating the derivation of these DS-1-like G8P[8] strains from a common ancestor. To our knowledge, this is the first report on full genome-based characterization of DS-1-like G8P[8] strains that have emerged in Thailand. Our observations will add to our growing understanding of the evolutionary patterns of emerging DS-1-like intergenogroup reassortant strains.

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Abrupt emergence and predominance in Vietnam of rotavirus A strains possessing a bovine-like G8 on a DS-1-like background

Abstract: An apparently single rotavirus A strain possessing a genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 abruptly emerged, caused diarrhoea in children requiring hospitalisation, and increased to reach 27 % of strains detected during the first half of 2015 in Vietnam.

Cited by 51 publications

References 12 publications

Predominant prevalence of human rotaviruses with the G1P[8] and G8P[8] genotypes with a short RNA profile in 2013 and 2014 in Sukhothai and Phetchaboon provinces, Thailand

Predominant prevalence of human rotaviruses with the G1P[8] and G8P[8] genotypes with a short RNA profile in 2013 and 2014 in Sukhothai and Phetchaboon provinces, Thailand

Clinical and Molecular Characteristics of Human Rotavirus G8P[8] Outbreak Strain, Japan, 2014

Full Genome Characterization of Novel DS-1-Like G8P[8] Rotavirus Strains that Have Emerged in Thailand: Reassortment of Bovine and Human Rotavirus Gene Segments in Emerging DS-1-Like Intergenogroup Reassortant Strains

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