Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Jing, Fuyuan; Zhang, Ling; Li, Meiya; Tang, Yueli; Wang, Yuliang; Wang, Yueyue; Wang, Quan; Pan, Qin; Wang, Guofeng; Tang, Kexuan

doi:10.2478/s11756-009-0040-8

Cited by 95 publications

(72 citation statements)

References 26 publications

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“…Although artemisinin was previously reported to be overproduced also after treatment with ABA (Jing et al 2009) and SA (Pu et al 2009), the issue of any increase in plant biomass was not addressed. The highest artemisinin contents in treated plants with ABA and SA were 1.84% DW and 1.4% DW, which was 65 and 75.8% higher than the respective controls (Jing et al 2009;Pu et al 2009). The application of salinity stress was claimed to be a simple and efficient procedure for boosting artemisinin content (2-3% DW).…”

Section: Discussionmentioning

confidence: 99%

“…Irradiation ), salinity stress (Qureshi et al 2005;Qian et al 2007), chilling stress (Feng et al 2009) and DMSO elicitation (Mannan et al 2010) have been reported to affect artemisinin production. In addition, phytohormones involved in the plant defense response [e.g., abscisic acid (Jing et al 2009) and salicylic acid (Pu et al 2009)] have also been shown to play important roles in artemisinin accumulation.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA3 treatment

2010

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Artemisinin is a promising and potent antimalarial drug naturally produced by the plant Artemisia annua L. but in very low yield. Its artemisinin content is known to be greatly affected by both genotype and environmental factors. In this study, the production of artemisinin and leaf biomass in Artemisia annua L. was significantly increased by exogenous GA 3 treatment. The effect of GA 3 application on expression of proposed key enzymes involved in artemisinin yield was examined in both wild type (007) and FPS-overexpression (253-2) lines of A. annua. In the wild type (007) at 6 h post GA 3 application there was an abrupt rise in FPS, ADS and CYP71AV1 expression and at 24 h a temporary and significant peak in artemisinin (1.45-fold higher than the control). After GA 3 application in line 253-2, there was a dramatic rise in expression of FPS at 3 h, CYP71AV1 at 9 h and ADS at 72 h and accumulation of artemisinin after 7 days, which was a delay when compared with the wild type plant. Thus, increased artemisinin content from exogenous GA 3 treatment was associated with increased expression of key enzymes in the artemisinin biosynthesis pathway. Interestingly, exogenous GA 3 continuously enhanced artemisinin content from the vegetative stage to flower initiation in both plant lines and gave significantly higher leaf biomass than in control plants. Consequently, the artemisinin yield in GA 3 -treated plants was much higher than in control plants. Although the maximum artemisinin content was found at the full blooming stage [2.1% dry weight (DW) in 007 and 2.4% DW in 253-2], the highest artemisinin yield in GA 3 -treated plants was obtained during the flower initiation stage (2.4 mg/plant in 007 and 2.3 mg/plant in 235-2). This was 26.3 and 27.8% higher, respectively, than in non-treated plants 007 and 253-2. This study showed that exogenous GA 3 treatment enhanced artemisinin production in pot experiments and should be suitable for field application.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA3 treatment

2010

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“…But most of these experiments were conducted on suspension or hairy root culture of A. annua and not on large growing plants as in the present study. Published results showed that ADS expression could be up-regulated by SA and methyl jasmonate (MJ), but with no response to DMSO; CPR could be up-regulated by ABA and miconazole, but showed no response to SA and MJ; CYP could be up-regulated by low concentrations of MJ (22 M), and DMSO, but showed no response to high concentration of MJ (100 M), SA and miconazole [10,[13][14][15][16]. The responses in expression of these three genes regulated by SA in our experiments were in agreement with published reports [13,14].…”

Section: Resultsmentioning

confidence: 99%

Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Yin¹,

Kjær²,

Fretté³

et al. 2012

Process Biochemistry

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“…ABA, defined as a stress hormone, plays a central role in responses to biotic and abiotic stresses [21]. It was reported that ABA could stimulate the accumulation of some secondary metabolites [22,23]. Under ABA treatment, the PgSTS expression level was increased to 3.56 fold at 2 hr and rapidly decreased to minimum value at 4 hr, and then the expression increased gradually to top point 4.56 fold at 48 hr post treatments (Fig.…”

Section: Differential Expressions Of the Pgsts Under Various Abiotic mentioning

confidence: 88%

Isolation of Sesquiterpene Synthase Homolog from Panax ginseng C.A. Meyer

Khorolragchaa¹,

Parvin²,

Shim³

et al. 2010

Journal of Ginseng Research

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: Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.

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Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Cited by 95 publications

References 26 publications

Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA3 treatment

Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA3 treatment

Chitosan oligosaccharide and salicylic acid up-regulate gene expression differently in relation to the biosynthesis of artemisinin in Artemisia annua L.

Isolation of Sesquiterpene Synthase Homolog from Panax ginseng C.A. Meyer

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