Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Ze, Nicolas Mfe'e; Cantliffe, Daniel J.

doi:10.1007/s11627-001-0047-5

Cited by 20 publications

(8 citation statements)

References 23 publications

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“…The influence of ABA on somatic embryos maturation has already reported in different plant species (Ackerson 1984, Girija et al 2000, Torres et al 2001, Ramakrishnan et al 2005. However, we report for the first time in mung bean the combination of KNO 3 -, PEG 6000, Pro, and ABA that showed enhanced production of mature embryos (46.5 %) when cultured in half-strength B5 medium.…”

Section: Discussionsupporting

confidence: 56%

Somatic embryogenesis and regeneration of Vigna radiata

Sivakumar¹,

Gnanam²,

Ramakrishnan³

et al. 2010

Biologia plant.

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An efficient regeneration protocol via somatic embryogenesis was optimized for mung bean [Vigna radiata (L.) Wilczek; cv. Vamban 1]. Primary leaf explants were used for embryogenic callus induction in MMS medium (Murashige and Skoog salts with B5 vitamins) containing 2.0 mg dm -3 2,4-dichlorophenoxyacetic acid (2,4-D), 150 mg dm -3 glutamine and 3 % sucrose. Fast growing, highly embryogenic cell suspensions were established from 21-d-old calli in MMS medium supplemented with 0.5 mg dm -3 2,4-D and 50 mg dm -3 proline (Pro), and maximum recovery of globular (39.0 %), heart-shaped (26.3 %) and torpedo-stage (21.0 %) somatic embryos were observed in this medium. Mature cotyledonary-stage somatic embryos were cultured for 5 d in half strength B5 liquid medium containing 0.05 mg dm -3 2,4-D, 20 mg dm -3 Pro, 5 μM abscisic acid, 1000 mg dm -3 KNO 3, 50 mg dm -3 polyethylene glycol (PEG 6000) and 30 g dm -3 D-mannitol. Mature somatic embryos were germinated after dessication for 3 d and complete development of plantlets accomplished in MMS medium containing 30 g dm -3 maltose, 0.5 mg dm -3 benzyladenine and 500 mg dm -3 KNO 3 . Profuse lateral roots, and regeneration frequency (up to 60 %) were observed in half-strength MMS medium containing 0.5 mg dm -3 indolebutyric acid (IBA). The regenerated plants were grown to fruiting and were morphologically normal and fertile.Additional key words: cell suspension cultures, mung bean, plant growth regulators, primary leaf explant.

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Section: Discussionsupporting

confidence: 56%

Somatic embryogenesis and regeneration of Vigna radiata

Sivakumar¹,

Gnanam²,

Ramakrishnan³

et al. 2010

Biologia plant.

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“…At present, the challenge encountered with the production of somatic embryos as 'synthetic seeds' is in the maturation stage (Ara et al 2000). A number of studies have reported effective methods by which to mature somatic embryos, and successful treatments include the addition of osmoticum (Ramarosandratana et al 2001), use of ethylene inhibitors (Mandal et al 2001), supplement of ABA (Torres et al 2001), stress by temporary starvation (Lee et al 2001) and desiccation (Gorbatenko and Hakman 2001).…”

Section: Induction Of Somatic Embryosmentioning

confidence: 99%

Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

Wang

Bhalla

2004

Plant Cell Reports

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Somatic embryogenesis from leaf explants of Scaevola aemula R. Br. was achieved. Somatic embryos were induced from explants cultured on MS medium supplemented with 0.2 mg/ 2,4-dichlorophenoxyacetic acid and 0.2-0.5 mg/l 6-benzylaminopurine (BAP). Various developmental stages of somatic embryos were found on this medium-from globular embryos to germinated embryos. The transfer of globular embryos to MS medium containing 0.5 mg/l BAP resulted in a high frequency of shoot regeneration. Leaf explants cultured on MS medium containing different combinations of BAP and alpha-naphthaleneacetic acid formed adventitious shoots and roots. Histological examination confirmed the process of somatic embryogenesis. Induction of somatic embryogenesis in Scaevola provides a system for studying embryogenesis in Australian native plants and will facilitate the improvement of these plants using genetic transformation techniques.

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“…An efficient, synchronous SE system is of particular importance for commercial micropropagation and for synthetic seeds production and physiological in vitro studies ( Al-Khayri and Al-Bahrany, 2012 ). Such system has recently been established in suspension culture in many species, i.e., carrots ( Osuga and Komamine, 1994 ), sweet potato ( Torres et al, 2001 ), date palm ( Phoenix dactylifera ) ( Al-Khayri and Al-Bahrany, 2012 ), and Douglas-fir ( Pseudotsuga menziesii ) ( Gupta and Timmis, 2005 ). Cultures grown in the liquid medium have shown characteristics of well dispersion, rapid growth, and good repeatability, and it is easier to realize the synchronous generation of somatic embryos than that on the solid medium ( Song et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Multiple Methods Synergistically Promote the Synchronization of Somatic Embryogenesis Through Suspension Culture in the New Hybrid Between Pinus elliottii and Pinus caribaea

Yao

et al. 2022

Front. Plant Sci.

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Pinus elliottii × Pinus caribaea is an interspecific pine hybrid of major economic importance. Somatic embryogenesis and plant regeneration in P. elliottii × P. caribaea on solid medium have been reported previously; however, a current limitation is the lack of a stable and effective method for its commercial use. The objective of this study was to establish a suspension culture system and evaluate the effect of multiple methods synergistically on the synchronization of embryo development in P. elliottii × P. caribaea. For the former, a protocol to initiate and establish a suspension culture system of P. elliottii × P. caribaea was presented. Based on biomass growth, the growth of embryogenic calli (EC) followed an S-shape curve in suspensions grown for a 15-day period, and the exponential phase of cell suspensions was reached between days 3 and 6. The initial packed cell volume (PCV) and revolutions per minute (rpm) have a significant effect on the proliferation of EC, and the highest proliferation multiple reached 6.86 (±0.06) at the initial density of 5 ml PCV under a 9–10 days transfer interval in the dark on a rotary shaker at 70 rpm. For the latter, the influence of abscisic acid (ABA), ammonium (NH4+), nitrate (NO3–), low temperature, and polyethylene glycol (PEG) on somatic embryogenesis was very significant. When EC were suspended in the medium at a presence of 37.84 μM/L ABA, as many as 274 mature cotyledonary embryos/ml PCV of cells were thereafter formed in the mature medium, and 266 somatic embryos were obtained on mature medium after suspension culture in liquid medium containing 10 mmol/L NH4+ and 30 mmol/L NO3–. Furthermore, reducing the concentration of 2,4-dichlorophenoxyacetic acid gradually and at 4°C incubation for 12 h in the initial exponential phase could promote the synchronization of somatic embryogenesis, which resulted in 260 mature cotyledonary embryos. This suspension culture system and method of synchronic control can be used in the large-scale production of P. elliottii × P. caribaea seedlings.

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Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Cited by 20 publications

References 23 publications

Somatic embryogenesis and regeneration of Vigna radiata

Somatic embryogenesis and regeneration of Vigna radiata

Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

Multiple Methods Synergistically Promote the Synchronization of Somatic Embryogenesis Through Suspension Culture in the New Hybrid Between Pinus elliottii and Pinus caribaea

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