Abscisic Acid Catabolism Generates Phaseic Acid, a Molecule Able to Activate a Subset of ABA Receptors

Rodriguez, Pedro L.

doi:10.1016/j.molp.2016.09.009

Cited by 21 publications

(15 citation statements)

References 12 publications

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“…ABA catabolic pathways are conventionally thought of as routes for ABA inactivation. Nevertheless, increasing reports imply that some ABA metabolites, such as phaseic acid (PA), possess ABA-like biological activity ranging from the regulation of stomata closure to inhibition of seed germination [ 59 , 60 ]. Eventually, PA is metabolically converted to dihydrophaseic acid (DPA), which is inactive in various bioassays [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Integrated transcriptome and hormone profiling highlight the role of multiple phytohormone pathways in wheat resistance against fusarium head blight

Wang

Liu

et al. 2018

PLoS ONE

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Fusarium head blight (FHB or scab) caused by Fusarium spp. is a destructive disease of wheat. Since the most effective sources of FHB resistance are typically associated with unfavorable agronomic traits, breeding commercial cultivars that combine desired agronomic traits and a high level of FHB resistance remains a considerable challenge. A better understanding of the molecular mechanisms governing FHB resistance will help to design more efficient and precise breeding strategies. Here, multiple molecular tools and assays were deployed to compare the resistant variety Sumai3 with three regionally adapted Canadian cultivars. Macroscopic and microscopic disease evaluation established the relative level of Type II FHB resistance of the four varieties and revealed that the F. graminearum infection process displayed substantial temporal differences among organs. The rachis was found to play a critical role in preventing F. graminearum spread within spikes. Large-scale, organ-specific RNA-seq at different times after F. graminearum infection demonstrated that diverse defense mechanisms were expressed faster and more intensely in the spikelet of resistant varieties. The roles of plant hormones during the interaction of wheat with F. graminearum was inferred based on the transcriptomic data obtained and the quantification of the major plant hormones. Salicylic acid and jasmonic acid were found to play predominantly positive roles in FHB resistance, whereas auxin and ABA were associated with susceptibility, and ethylene appeared to play a dual role during the interaction with F graminearum.

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Section: Resultsmentioning

confidence: 99%

Integrated transcriptome and hormone profiling highlight the role of multiple phytohormone pathways in wheat resistance against fusarium head blight

Wang

Liu

et al. 2018

PLoS ONE

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“…A transgenic approach comparing at least 2 different inactivation pathways is also important, since metabolic engineering carries a risk of phenotypic artifacts from the degradation products (van Wees and Glazebrook, 2003). Recently, the CYP707A ABA degradation product phaseic acid was found to be active in both mammalian and plant cells, which could cause such phenotypic artifacts (Hou et al, 2016; Rodriguez, 2016; Weng et al, 2016). This means that future ABA depletion studies relying on CYP707A activity should most likely also express the phaseic acid reductase (PAR, Arabidopsis ABH2: At4g27250) to avoid one potential side effect.…”

Section: Perspectives: How Plant Science and Studies Of Phytopathogenmentioning

confidence: 99%

Abscisic Acid as Pathogen Effector and Immune Regulator

et al. 2017

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Abscisic acid (ABA) is a sesquiterpene signaling molecule produced in all kingdoms of life. To date, the best known functions of ABA are derived from its role as a major phytohormone in plant abiotic stress resistance. Different organisms have developed different biosynthesis and signal transduction pathways related to ABA. Despite this, there are also intriguing common themes where ABA often suppresses host immune responses and is utilized by pathogens as an effector molecule. ABA also seems to play an important role in compatible mutualistic interactions such as mycorrhiza and rhizosphere bacteria with plants, and possibly also the animal gut microbiome. The frequent use of ABA in inter-species communication could be a possible reason for the wide distribution and re-invention of ABA as a signaling molecule in different organisms. In humans and animal models, it has been shown that ABA treatment or nutrient-derived ABA is beneficial in inflammatory diseases like colitis and type 2 diabetes, which confer potential to ABA as an interesting nutraceutical or pharmacognostic drug. The anti-inflammatory activity, cellular metabolic reprogramming, and other beneficial physiological and psychological effects of ABA treatment in humans and animal models has sparked an interest in this molecule and its signaling pathway as a novel pharmacological target. In contrast to plants, however, very little is known about the ABA biosynthesis and signaling in other organisms. Genes, tools and knowledge about ABA from plant sciences and studies of phytopathogenic fungi might benefit biomedical studies on the physiological role of endogenously generated ABA in humans.

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“…For example, the ABA degradation by-product, phaseic acid (PA), is able to bind to certain ABA receptors (Weng et al , 2016). PA has submicromolar activity towards the ABA receptors PYL3, PYL5, and PYL6, and a role for PA on water stress tolerance has been proposed (Lozano-Juste and Cutler, 2016; Rodriguez, 2016; Weng et al , 2016). Other hormone receptors can also accommodate hormone analogs or different forms of the hormone.…”

Section: Discussionmentioning

confidence: 99%

The fungal sesquiterpenoid pyrenophoric acid B uses the plant ABA biosynthetic pathway to inhibit seed germination

Lozano‐Juste

Masi²,

Cimmino³

et al. 2019

Journal of Experimental Botany

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Pyrenophoric acid (P-Acid), P-Acid B, and P-Acid C are three phytotoxic sesquiterpenoids produced by the ascomycete seed pathogen Pyrenophora semeniperda, a fungus proposed as a mycoherbicide for biocontrol of cheatgrass, an extremely invasive weed. When tested in cheatgrass bioassays, these metabolites were able to delay seed germination, with P-Acid B being the most active compound. Here, we have investigated the cross-kingdom activity of P-Acid B and its mode of action, and found that it activates the abscisic acid (ABA) signaling pathway in order to inhibit seedling establishment. P-Acid B inhibits seedling establishment in wild-type Arabidopsis thaliana, while several mutants affected in the early perception as well as in downstream ABA signaling components were insensitive to the fungal compound. However, in spite of structural similarities between ABA and P-Acid B, the latter is not able to activate the PYR/PYL family of ABA receptors. Instead, we have found that P-Acid B uses the ABA biosynthesis pathway at the level of alcohol dehydrogenase ABA2 to reduce seedling establishment. We propose that the fungus P. semeniperda manipulates plant ABA biosynthesis as a strategy to reduce seed germination, increasing its ability to cause seed mortality and thereby increase its fitness through higher reproductive success.

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Abscisic Acid Catabolism Generates Phaseic Acid, a Molecule Able to Activate a Subset of ABA Receptors

Cited by 21 publications

References 12 publications

Integrated transcriptome and hormone profiling highlight the role of multiple phytohormone pathways in wheat resistance against fusarium head blight

Integrated transcriptome and hormone profiling highlight the role of multiple phytohormone pathways in wheat resistance against fusarium head blight

Abscisic Acid as Pathogen Effector and Immune Regulator

The fungal sesquiterpenoid pyrenophoric acid B uses the plant ABA biosynthetic pathway to inhibit seed germination

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