Abscisic acid levels in seeds of the gibberellin‐deficient mutant <i>lh‐2</i> of pea (<i>Pisum sativum</i>)

Batge, Shona L.; Ross, John J.; Reid, James B.

doi:10.1034/j.1399-3054.1999.105313.x

Cited by 23 publications

(17 citation statements)

References 28 publications

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“…Calculations were performed as described by McKay et al. (1994) and Batge et al. (1999) following correction for isotopic impurity in the ABA internal standard.…”

Section: Methodsmentioning

confidence: 99%

Pea rms6 mutants exhibit increased basal branching

Rameau

Murfet

Laucou

et al. 2002

Physiologia Plantarum

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Our studies on two branching mutants of pea (Pisum sativum L.) have identified a further Ramosus locus, Rms6, with two recessive or partially recessive mutant alleles: rms6-1 (type line S2-271) and rms6-2 (type line K586). Mutants rms6-1 and rms6-2 were derived from dwarf and tall cultivars, Solara and Torsdag, respectively. The rms6 mutants are characterized by increased branching from basal nodes. In contrast, mutants rms1 through rms5 have increased branching from both basal and aerial (upper stem) nodes. Buds at the cotyledonary node of wild-type (WT) plants remain dormant but in rms6 plants these buds were usually released from dormancy. Their growth was either subsequently inhibited, sometimes even prior to emergence above ground, or they grew into secondary stems. The mutant phenotype was strongest for rms6-1 on the dwarf background. Although rms6-2 had a weak single-mutant phenotype, the rms3-1 rms6-2 double mutant showed clear transgression and an additive branching phenotype, with a total lateral length almost 2-fold greater than rms3-1 and nearly 5-fold greater than rms6-2. Grafting studies between WT and rms6-1 plants demonstrated the primary action of Rms6 may be confined to the shoot. Young WT and rms6-1 shoots had similar auxin levels, and decapitated plants had a similar magnitude of response to applied auxin. Abscisic acid levels were elevated 2-fold at node 2 of young rms6-1 plants. The Rms6 locus mapped to the R to Gp segment of linkage group V (chromosome 3). The rms6 mutants will be useful for basic research and also have possible agronomical value.

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“…Calculations were performed as described by McKay et al. (1994) and Batge et al. (1999) following correction for isotopic impurity in the ABA internal standard.…”

Section: Methodsmentioning

confidence: 99%

Pea rms6 mutants exhibit increased basal branching

Rameau

Murfet

Laucou

et al. 2002

Physiologia Plantarum

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“…Although incomplete genome coverage can make it more difficult to conclude gene absence than presence from sequence data, this observation suggests that a specialized ABA2 enzyme for catalysis of this step of ABA biosynthesis likely evolved after divergence of the gymnosperm and angiosperm lineages. The conversion of xanthoxin to abscisic aldehyde can be catalyzed to a limited degree by SDRs other than ABA2 in angiosperms, as evidenced by the residual level of ABA produced in aba2 null mutants (Batge et al ; González‐Guzmán et al ; McAdam et al ). However, these non‐specific enzymes are slower at catalyzing this step, as evidenced by the reduced speed at which ABA levels increase in drought‐stressed Pisum aba2 mutant plants (Wang et al ; McAdam et al ).…”

Section: Gymnospermsmentioning

confidence: 99%

What are the evolutionary origins of stomatal responses to abscisic acid in land plants?

Sussmilch

Brodribb

McAdam

2017

JIPB

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The evolution of active stomatal closure in response to leaf water deficit, mediated by the hormone abscisic acid (ABA), has been the subject of recent debate. Two different models for the timing of the evolution of this response recur in the literature. A single-step model for stomatal control suggests that stomata evolved active, ABAmediated control of stomatal aperture, when these structures first appeared, prior to the divergence of bryophyte and vascular plant lineages. In contrast, a gradualistic model for stomatal control proposes that the most basal vascular plant stomata responded passively to changes in leaf water status. This model suggests that active ABA-driven mechanisms for stomatal responses to water status instead evolved after the divergence of seed plants, culminating in the complex, ABA-mediated responses observed in modern angiosperms. Here we review the findings that form the basis for these two models, including recent work that provides critical molecular insights into resolving this intriguing debate, and find strong evidence to support a gradualistic model for stomatal evolution.

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“…IAA was extracted from frozen tissue (0.03 to 0.5 g) as described by Morris et al (2001), based on a modification of Batge et al (1999).…”

Section: Endogenous Iaa Analysismentioning

confidence: 99%

The Branching Gene RAMOSUS1 Mediates Interactions among Two Novel Signals and Auxin in Pea

et al. 2005

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In Pisum sativum, the RAMOSUS genes RMS1, RMS2, and RMS5 regulate shoot branching via physiologically defined mobile signals. RMS1 is most likely a carotenoid cleavage enzyme and acts with RMS5 to control levels of an as yet unidentified mobile branching inhibitor required for auxin inhibition of branching. Our work provides molecular, genetic, and physiological evidence that RMS1 plays a central role in a shoot-to-root-to-shoot feedback system that regulates shoot branching in pea. Indole-3-acetic acid (IAA) positively regulates RMS1 transcript level, a potentially important mechanism for regulation of shoot branching by IAA. In addition, RMS1 transcript levels are dramatically elevated in rms3, rms4, and rms5 plants, which do not contain elevated IAA levels. This degree of upregulation of RMS1 expression cannot be achieved in wild-type plants by exogenous IAA application. Grafting studies indicate that an IAA-independent mobile feedback signal contributes to the elevated RMS1 transcript levels in rms4 plants. Therefore, the long-distance signaling network controlling branching in pea involves IAA, the RMS1 inhibitor, and an IAA-independent feedback signal. Consistent with physiological studies that predict an interaction between RMS2 and RMS1, rms2 mutations appear to disrupt this IAA-independent regulation of RMS1 expression.

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Abscisic acid levels in seeds of the gibberellin‐deficient mutant lh‐2 of pea (Pisum sativum)

Cited by 23 publications

References 28 publications

Pea rms6 mutants exhibit increased basal branching

Pea rms6 mutants exhibit increased basal branching

What are the evolutionary origins of stomatal responses to abscisic acid in land plants?

The Branching Gene RAMOSUS1 Mediates Interactions among Two Novel Signals and Auxin in Pea

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