Abscisic Acid Synthesis and Response

Finkelstein, Ruth

doi:10.1199/tab.0166

Cited by 875 publications

(834 citation statements)

References 406 publications

(417 reference statements)

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“…In our study, four SnRKs (At2g26980, At4g33950, At4g24400, and At1g10940) were up-regulated significantly (Table II). In addition, the expression level of ABI5 (At2g36270), which was identified as a transcriptional regulator mediating ABA responses in seed germination and development (Finkelstein, 2013), was also elevated (Table II), while ABSCISIC ACID HYPERSENSITIVE1 (At2g13540), an mRNA cap-binding protein that negatively modulates early ABA signal transduction, showed decreased expression (Table II). Taken together, we assume that the signaling cascades of ABA increased in SSE seeds apparently due to the lower water contents and enhanced desiccation found in these seeds (Fig.…”

Section: Microarray Analysis Reveals the Induction Of Stress-associatmentioning

confidence: 99%

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Seed-Specific Expression of a Feedback-Insensitive Form of CYSTATHIONINE-γ-SYNTHASE in Arabidopsis Stimulates Metabolic and Transcriptomic Responses Associated with Desiccation Stress

et al. 2014

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and Tel-Hai College, Upper Galilee 11016, Israel (R.A.) With an aim to elucidate novel metabolic and transcriptional interactions associated with methionine (Met) metabolism in seeds, we have produced transgenic Arabidopsis (Arabidopsis thaliana) seeds expressing a feedback-insensitive form of CYSTATHIONINEg-SYNTHASE, a key enzyme of Met synthesis. Metabolic profiling of these seeds revealed that, in addition to higher levels of Met, the levels of many other amino acids were elevated. The most pronounced changes were the higher levels of stress-related amino acids (isoleucine, leucine, valine, and proline), sugars, intermediates of the tricarboxylic acid cycle, and polyamines and lower levels of polyols, cysteine, and glutathione. These changes reflect stress responses and an altered mitochondrial energy metabolism. The transgenic seeds also had higher contents of total proteins and starch but lower water contents. In accordance with the metabolic profiles, microarray analysis identified a strong induction of genes involved in defense mechanisms against osmotic and drought conditions, including those mediated by the signaling cascades of ethylene and abscisic acid. These changes imply that stronger desiccation processes occur during seed development. The expression levels of transcripts controlling the levels of Met, sugars, and tricarboxylic acid cycle metabolites were also significantly elevated. Germination assays showed that the transgenic seeds had higher germination rates under salt and osmotic stresses and in the presence of ethylene substrate and abscisic acid. However, under oxidative conditions, the transgenic seeds displayed much lower germination rates. Altogether, the data provide new insights on the factors regulating Met metabolism in Arabidopsis seeds and on the mechanisms by which elevated Met levels affect seed composition and behavior.

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Section: Microarray Analysis Reveals the Induction Of Stress-associatmentioning

confidence: 99%

“…The ABA-insensitive mutant abi4 was used as a control (Finkelstein, 2013). In the presence of ABA, the abi4 mutant germinated much faster than the wild-type and the transgenic seeds.…”

Section: Germination Rates Of Transgenic Seeds Under Stress and Hormomentioning

confidence: 99%

Seed-Specific Expression of a Feedback-Insensitive Form of CYSTATHIONINE-γ-SYNTHASE in Arabidopsis Stimulates Metabolic and Transcriptomic Responses Associated with Desiccation Stress

et al. 2014

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“…İlk çalışmalar dışarıdan ABA uygulamasının kesik yapraklarda senesensi teşvik ettiğini göstermiştir ancak kesik olmayan yapraklarda bu etki daha azdır [8]. Kuraklık, yüksek tuz konsantrasyonu, düşük sıcaklık gibi çevresel stres durumları sıklıkla yaprak senesensini teşvik eder ve bitkiler bu şartlara maruz kalınca yapraklardaki ABA konsantrasyonu artar [42]. Genetik çalışmalar, ABA biyosentezi ya da sinyal yolu eksik çeşitli Arabidopsis mutantlarında değiştirilmiş yaprak senesensi fenotipleri açığa çıktığını göstermektedir [8].…”

Section: Absisik Asitunclassified

Yaprak Senesensi: Fizyolojik ve Moleküler Düzenlenmesine Bakış

Sağlam

2015

Mar. J. Pure Appl. Sci.

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ÖZETBitkilerde görülen, tüm bitki, organ, doku ya da hücre ölümü ile sonuçlanan önemli bir gelişme süreci olan senesens ile ilgili çalışmalar 1800'lü yılların sonlarına dayanmaktadır. İlk yıllarda gözlemlerden oluşan bu çalışmalar, günümüzde teknolojinin ve genetik biliminin gelişmesiyle yerini kapsamlı biyoteknolojik araştırmalara bırakmıştır. Dünyanın çeşitli yerlerindeki araştırma merkezlerinde bilim adamları senesensin genetik mekanizmasının aydınlatılması ile ilgili deneyler yapmaktadırlar. Üzerinde en çok araştırma yapılan senesens çeşidi, yapraklarda görülen ve klorofil kaybı ile karakterize edilen yaprak senesensidir. Senesens mekanizmasının aydınlatılması ekonomik düzeyde ürün kalitesinin artırılmasında, maydanoz, brokoli, ıspanak gibi sebze, domates, elma gibi meyve ve karanfil, Alstroemeria, petunya gibi süs bitkilerinin raf ömrünün uzatılmasında büyük yararlar sağlayacaktır. Bu derlemede yaprak senesensinin fizyolojik ve moleküler düzenlenme mekanizmaları anlatılmıştır.Anahtar kelimeler: Yaprak senesensi, klorofil kaybı, genetik mekanizma, fizyolojik düzenlenme I. GİRİŞSenesens yaşa bağlı olarak hücre, doku, organ ve organizma düzeyinde ölümle ya da yaşam döngüsünün sona ermesiyle sonuçlanan bitki gelişimindeki son evredir [1,2,3,4]. Azot, kükürt, fosfor ve potasyum gibi önemli besinlerin taşınması ve geri dönüşümü açısından bir bitkinin gelişiminde senesens hayati önem taşımaktadır. Bu besinler senesens olan yapraklardan aktif olarak büyüyen dokulara taşınır, böylece bitkinin büyümesi ve çoğalması desteklenmiş olur [3,4]. Bazı araştırıcılar senesensin, elverişsiz çevre koşulları (kuraklık, sıcaklık, azot eksikliği, yetersiz ışık, hastalık ve patojen saldırılar) sonucunda meydana gelmesinin [5] yanında, en uygun büyüme koşullarında yetişen sağlıklı bitkilerde de genetik olarak meydana geldiğini bildirmişlerdir [6,7]. Bir hücrenin yaşamsal geçmişi mitotik ve post-mitotik süreçlerden meydana gelir. Buna bağlı olarak bitkilerin mitotik ve post-mitotik senesens gösterdiği tanımlanmıştır (Şekil 1). Mitotik senesens (proliferatif senesens) olarak da bilinmektedir ve germline benzeri meristem hücrelerinin mitotik hücre bölünmesine maruz kalma yeteneklerini kaybettikleri zaman meydana gelir. Kültürdeki insan hücreleri ve mayada görülen replikatif senesensin aksine bitkilerdeki mitotik senesens telomer kısalması tarafından kontrol edilmemektedir. Post-mitotik senesens aktif bir bozulma sürecidir, yapraklar ve çiçek petalleri gibi organlarda meydana gelmektedir [8]. Leaf Senescence: A View of Its Physiological and Molecular RegulationAbSTRACT Studies related to senescence, which is an important developmental process in plants and leads to death of a cell, tissue or whole plant, is based on the end of the 1800s. The preliminary studies were at observational level but they were replaced by comprehensive biotechnological research in conjunction with the development of technology and genetic science. The scientists in various part of the world have carried out experiments related to elucidation of the genetic mech...

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“…The phytohormone abscisic acid (ABA) fulfills pivotal roles in plant development and stress responses. 1 Cellular dehydration during seed maturation or under stress conditions upregulates endogenous ABA levels, which activate many ABA-responsive genes with G-box-like cis -acting ABA-responsive elements (ABREs, PyACGTGG/TC) in their promoter regions. 1,2 In Arabidopsis , binding of the PYR/PYL/RCAR receptor proteins to ABA triggers the formation of ternary complexes with group-A protein phosphatase 2Cs (PP2Cs) and derepresses the subclass III SnRK2 protein kinases such as SRK2D/SnRK2.2, SRK2E/OST1/SnRK2.6, SRK2I/SnRK2.3.…”

mentioning

confidence: 99%

“…1 Cellular dehydration during seed maturation or under stress conditions upregulates endogenous ABA levels, which activate many ABA-responsive genes with G-box-like cis -acting ABA-responsive elements (ABREs, PyACGTGG/TC) in their promoter regions. 1,2 In Arabidopsis , binding of the PYR/PYL/RCAR receptor proteins to ABA triggers the formation of ternary complexes with group-A protein phosphatase 2Cs (PP2Cs) and derepresses the subclass III SnRK2 protein kinases such as SRK2D/SnRK2.2, SRK2E/OST1/SnRK2.6, SRK2I/SnRK2.3. 3–7 Then, the SnRK2s phosphorylate basic leucine zipper (bZIP) transcription factors such as ABRE-binding protein/ABRE-binding factors (AREB/ABFs), which then activate the expression of ABA-responsive genes.…”

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confidence: 99%

Protein kinase CK2α subunits constitutively activate ABA signaling in Arabidopsis

Nagatoshi

Fujita

2018

Plant Signaling & Behavior

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Protein kinase CK2 (formerly known as casein kinase II), a Ser/Thr protein kinase highly conserved in eukaryotes, is essential for cell survival by regulating a wide range of plant growth, development, and stress responses. A growing body of evidence has shown a link between CK2 and abscisic acid (ABA) signaling in response to abiotic stress. However, the roles of CK2 subunits in ABA signaling remain unclear in plants. Our recent work in Arabidopsis thaliana has revealed that CK2α and CK2β subunits inversely modulate ABA signal output. Here, we examine the roles of CK2αs, by assessing how CK2αs affect ABA signaling. Together with the previous findings, our mutant and transient expression analyses demonstrate that CK2αs positively modulate ABA signaling through the core ABA signaling pathway in the presence of ABA, though the positive effect of CK2αs are much smaller than that of core ABA signaling components in ABA response. In addtion, our current and previous findings also suggest that CK2αs play a role in maintaining constitutively active ABA signaling even in the absence of ABA independently of the core ABA signaling pathway. Thus, we found that CK2αs constitutively activate ABA signaling in the presence or absence of ABA in a different manner in Arabidopsis plants.

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Abscisic Acid Synthesis and Response

Cited by 875 publications

References 406 publications

Seed-Specific Expression of a Feedback-Insensitive Form of CYSTATHIONINE-γ-SYNTHASE in Arabidopsis Stimulates Metabolic and Transcriptomic Responses Associated with Desiccation Stress

Seed-Specific Expression of a Feedback-Insensitive Form of CYSTATHIONINE-γ-SYNTHASE in Arabidopsis Stimulates Metabolic and Transcriptomic Responses Associated with Desiccation Stress

Yaprak Senesensi: Fizyolojik ve Moleküler Düzenlenmesine Bakış

Protein kinase CK2α subunits constitutively activate ABA signaling in Arabidopsis

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