Absence of Human Papillomavirus DNA in Nongenital Seborrheic Keratosis

Abstract

Absence of Human Papillomavirus DNA in Nongenital Seborrheic KeratosisSeborrheic keratosis (SK) is a benign epidermal tumor of unknown etiology. Because of its wart-like morphology, human papillomavirus (HPV) has been suggested as a possible causative agent. Viral involvement, however, has not been confirmed yet despite extensive research. The aim of this study was to evaluate the presence of HPV 6/11, 31, 33 DNA in nongenital SK. We analyzed 40 biopsy specimens taken from patients with nongenital SK using in situ polymerase chain reaction (PCR) and PCR with tissue extracts. The SK specimens (n=40), analyzed by in situ PCR, were negative for all HPV probes tested (types 6/11, 31, 33). Control slides (condyloma acuminatum, n=3) were positive for type 6/11, 31, and 33 HPV probes tested. Melasma samples (n=4), the negative controls, were consistently negative. No HPV DNA band was detected by PCR with the tissue extracts from paraffin-embedded SK samples, while condyloma acuminatum, the positive controls, showed DNA bands of the correct molecular weights. Our results show that HPV type 6/11, 31, and 33 cannot be recognized as causative agents for nongenital SK, which is in contrast to the previous studies. Further studies are required to reveal the presence of other types (more than 90) of HPV DNA.

Epidermal growth factor receptor expression in mice skin upon ultraviolet B exposure - Seborrheic Keratosis as a coincidental and unique finding

“…This however is in disagreement with previous studies in which they described the etiology of SK as unknown,[17–20] but it is in agreement with Haw et al . [21] This study was the only research on UVB as a causative agent in inducing SK.…”

No evidence of human papillomaviruses in non-genital seborrheic keratosis

“…This result is similar to the results of the following studies, which reported the absence of HPVs genome: Lee et al ., reported that HPV types 6/11, 31, and 33 were not found in non-genital SK. [8] Also, 28 (96%) cases of non-genital SK did not contain HPVs DNA sequences in Zhu's study. [9] It is interesting to note that HPVs detection by IHC in all of the 31 inverted follicular keratoses (IFK) samples performed by Asadi-Amoli revealed negative result.…”

Detection of epidermodysplasia verruciformis-associated human papillomavirus DNA in nongenital seborrhoeic keratosis

“…Some EV-associated HPVs have been suggested to be associated with a diverse range of skin malignancies and premalignant diseases, 1,2 especially in immunocompromised patients. 3 According to their phylogenetic similarity, EV-associated HPVs consist of HPV 5,8,9,12,14,15,17,[19][20][21][22][23][24][25][36][37][38]47 and 49. 4 Using highly sensitive polymerase chain reaction (PCR), several unknown types belonging or related to EV-associated HPVs have been reported in renal transplant recipients (RTRs).…”