Absence of hyaluronidase in cultured human skin fibroblasts

Arbogast, Bradley W.; Hopwood, John J.; Dorfman, Albert

doi:10.1016/0006-291x(75)90326-5

Cited by 51 publications

(23 citation statements)

References 8 publications

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“…Further, there was no evidence of macromolecular degradation, which is consistent with the previously recognized absence of hyaluronidase in these cells ( 15).…”

Section: Methodssupporting

confidence: 90%

“…Treatment periods were usually 24 h unless indicated otherwise. This was followed by a 24-h labeling period, at the beginning of which fresh medium supplemented with [3H]acetate (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) MCi/ml), [3Hlglucosamine (1-2 tlCi/ ml), or [35S]sulfuric acid (10-25 ,Ci/ml) was added. Labeling periods were shortened in the time course experiments and in the pulse-chase study as indicated in the figure legends.…”

Section: Methodsmentioning

confidence: 99%

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n-Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts.

Smith¹

1987

J. Clin. Invest.

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The effects of the short-chain aliphatic carboxylic acid, n-butyrate, on glycosaminoglycan (GAG) accumulation were studied in cultured human skin fibroblasts. Normal fibroblast cultures were grown to confluence, shifted to a medium without or with n-butyrate for 24 h, labeled with either VHlacetate or V3Hjglucosamine and analyzed for IHIGAG and V3Hjhyaluronate accumulation. Accumulation was stimulated at low concentrations (0.1-1 mM) by up to 27%. Higher concentrations of n-butyrate (> 1 mM) inhibited V3HIGAG by up to 70-90%. This effect was maximal at 10 mM and half-maximal at 3 mM. Propionate had similar effects but was less potent. Parallel studies conducted in colonic fibroblasts revealed that n-butyrate could markedly inhibit I3HIGAG accumulation in that cell type as well. These effects were rapid, occurring within 3 h of treatment, and were reversible. Chondroitin sulfate accumulation was unaffected by the compound. A pulse-chase study failed to demonstrate any effect on 13HIGAG degradation.

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“…Further, there was no evidence of macromolecular degradation, which is consistent with the previously recognized absence of hyaluronidase in these cells ( 15).…”

Section: Methodssupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

n-Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts.

Smith¹

1987

J. Clin. Invest.

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“…The results shown in Table I the chase incubation (data not shown), which is consistent with the previously reported absence of hyaluronidase in human skin fibroblasts (19). These data suggest that Dex inhibition is the consequence of an attenuated rate of synthesis paralleling those observations made previously of T3 effects on GAG accumulation (10).…”

Section: Hourssupporting

confidence: 91%

Dexamethasone regulation of glycosaminoglycan synthesis in cultured human skin fibroblasts. Similar effects of glucocorticoid and thyroid hormones.

Smith¹

1984

J. Clin. Invest.

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“…However, the processes that regulate cellular HAase activity and the effect of alterations in HAase activity on tissue HA accumulation are poorly understood. In addition, since normal human fibroblasts have been reported to lack HAases (31,32), the role of fibroblast-derived HAase in tissue repair and remodeling in humans has not been analyzed.…”

mentioning

confidence: 99%