Absence of Intercellular Antigens in the Deep Layers of the Epidermis in Pemphigus Foliaceus

Bystryn, Jean‐Claude; Rodrı́guez, José Luis

doi:10.1172/jci108944

Cited by 46 publications

(15 citation statements)

References 21 publications

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“…There is some experimental support, which is derived from indirect immunofluorescence studies on PF lesions, for this hypothesis (31). However, the present study only found one PF serum that contained antibodies that were directed against PV antigen, and that serum also contained antibodies that were directed against an antigen defined by other PF sera.…”

Section: Resultssupporting

confidence: 50%

“…Another explanation for the different clinical and histologic expression of PV and PF is that perhaps PF and PV antibodies have the same specificity, but the reactive antigen is missing in the deeper epidermis of patients with PF (31). There is some experimental support, which is derived from indirect immunofluorescence studies on PF lesions, for this hypothesis (31).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceus autoantibodies.

Stanley¹,

Koulu²,

Thivolet³

1984

J. Clin. Invest.

229

102

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Section: Resultssupporting

confidence: 50%

Section: Resultsmentioning

confidence: 99%

Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceus autoantibodies.

Stanley¹,

Koulu²,

Thivolet³

1984

J. Clin. Invest.

229

102

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“…Immunofluorescence blocking studies have suggested that antibodies from patients with Brazilian pemphigus foliaceus may have different specificities than antibodies of patients with pemphigus vulgaris (31), however inability of one serum to block the binding of another serum does not prove that these sera bind to different molecules. The presence of several cell surface pemphigus antigens has also been suggested by the observation that different pemphigus sera tested on the same skin specimen may result in different immunofluorescence patterns (32,33). For example, some patients with pemphigus foliaceus have antibodies that only bind the superficial layers of normal epidermis (32).…”

Section: Resultsmentioning

confidence: 97%

“…For example, some patients with pemphigus foliaceus have antibodies that only bind the superficial layers of normal epidermis (32). However, most patients with pemphigus foliaceus have antibodies that bind all layers of normal epidermis and it has been suggested that a cell surface antigen, normally present throughout the epidermis, is missing in the lower part of the epidermis in pemphigus foliaceus lesions (33). Thus, the superficial or deep location of the blister in pemphigus foliaceus and pemphigus vulgaris, respectively, may be due either to distinct antigenic specificity of antibodies or to a different distribution, in the epidermis, of a single reactive antigen.…”

Section: Resultsmentioning

confidence: 99%

Pemphigus Antibodies Identify a Cell Surface Glycoprotein Synthesized by Human and Mouse Keratinocytes

Stanley¹,

Yaar²,

Hawley‐Nelson³

et al. 1982

J. Clin. Invest.

196

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A B S T R A C T Pemphigus is an antibody-mediated autoimmune skin disease in which loss of cell-to-cell contacts in the epidermis results in blister formation. Patients with pemphigus develop antibodies that bind to the keratinocyte cell surface, the site of primary pathology. The purpose of this study was to characterize the antigen(s) to which pemphigus antibodies bind. Because we could detect pemphigus antigen by indirect immunofluorescence on the surface of multiply-passaged cells in cultures of both a spontaneously transformed mouse keratinocyte cell line (Pam) and normal human epidermal cells, we used these cells as a source of antigen. In order to demonstrate biosynthesis of antigen and to characterize the antigen(s), we radiolabeled cell cultures with ['4C]glucosamine or D-[2-3H]mannose and used different pemphigus sera to immunoprecipitate antigen from nonionic detergent extracts of these labeled cells. Specifically precipitated radiolabeled molecules were identified using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and fluorography. Sera from five of seven pemphigus patients specifically precipitated (from extracts of both Pam cells and human epidermal cells) a molecule that, when reduced, was -130 kD, whereas seven normal human sera and two pemphigoid sera did not precipitate this molecule. The findings that (a) these precipitated molecules comigrated on SDS-PAGE and that (b) the 130-kD molecule could no longer be precipitated from cell extracts that had been previously reacted with a pemphigus serum, indicate that reactive pemphigus sera bind the same molecule. The molecule was not detected in the culture medium of these cells. This finding, along with the cell surface

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“…In fact, in some cases, PF antibodies have been reported to be located in the superficial epidermis only (14,15).…”

Section: Discussionmentioning

confidence: 99%

Identification of pemphigus vulgaris antigen extracted from normal human epidermis and comparison with pemphigus foliaceus antigen.

Eyre

Stanley²

1988

J. Clin. Invest.

196

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Immunoprecipitations of cultured keratinocyte extracts have shown that pemphigus vulgaris (PV) sera bind a polypeptide of 210,000 mol wt with disulfide-linked chains of 130,000 and 85,000 mol wt. To identify proteins in normal human skin recognized by PV antibodies, we performed immunoprecipitations of normal human epidermal extracts. All 22 PV sera tested immunoprecipitated a complex of polypeptides (PV complex) of 210,000, 130,000, and 85,000 mol wt, after reduction. One-and two-dimensional gel electrophoresis showed that the 130,000-and 85,000-mol-wt polypeptides of the PV antigen from both cultured keratinocytes and epidermis have identical charges and sizes. In addition to precipitating the PV complex, 14 of 22 PV sera also have antibodies to a calciumsensitive epitope on a different complex of polypeptides (PF complex) which has previously been shown to be precipitated by all pemphigus foliaceus (PF) sera. The PF complex consists of polypeptides of 260,000, 160,000, 110,000, and 85,000 mol wt. Although the majority of PV sera also precipitate the PF complex, no PF sera precipitate the PV complex. Thus, PV and PF can be absolutely distinguished on a molecular level using the patients' autoantibodies. The PV and PF complexes, although distinct, have certain similarities. The 85,000-mol-wt polypeptide of each is identical. The 160,000-mol wt-peptide of the PF complex and the 130,000-mol-wt peptide of the PV complex have the same isoelectric point and both are capable of disulfide linkage to the 85,000-mol-wt polypeptide. The PV and PF complexes are closely related and may prove important in cell adhesion.

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Absence of Intercellular Antigens in the Deep Layers of the Epidermis in Pemphigus Foliaceus

Cited by 46 publications

References 21 publications

Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceus autoantibodies.

Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceus autoantibodies.

Pemphigus Antibodies Identify a Cell Surface Glycoprotein Synthesized by Human and Mouse Keratinocytes

Identification of pemphigus vulgaris antigen extracted from normal human epidermis and comparison with pemphigus foliaceus antigen.

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