Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster

Crain, William R.; Eden, Francine C.; Pearson, William R.; Davidson, Eric H.; Britten, Roy J.

doi:10.1007/bf00292953

Cited by 94 publications

(33 citation statements)

References 30 publications

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“…Robertson (11) found mariner-like elements in 15% of 400 insect species from eight orders using degenerated PCR from conserved amino acid regions of the putative transposase. Previous studies have shown that the D. pulchellus, E. vuilleti, and A. mellifera genomes contain few interspersed repeated sequences, suggesting that transposable elements might be eliminated by male haploidy (7,8). Those data indicate that transposable elements with no direct deleterious effects on insect viability might not be eliminated by selection in male haploids during evolution.…”

Section: _ X ĩ G a C C G A T A C A A C A M A -R C C A C A T T G A C Cmentioning

confidence: 75%

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Mariner-like elements in hymenopteran species: insertion site and distribution.

Bigot¹,

Hamelin²,

Capy³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Copies of mariner-like element (MLE) transposons in two species, the bumble bee, Bombus terrestis, and the ant, Myrmica ruginodis, were sequenced. The hfll-sized elements are 1250 bp long in both species and include 28-bp inverted terminal repeats. The five copies sequenced were -75% similar to a mariner element (peach) of Drosophila maurina. The distribution of MLE in 27 hymenopteran species was studied by PCR and Southern blot hybridization; 93% of the species contained one or more of the four major forms of the element. They are inserted in their host genomes, in the middle ofa degenerated 30-bp palindrome, which is itself located in an 85-bp conserved region with a purine-rich tail at one of Its ends. The genomes of two parasitoid wasps, Diadromus pulchellus and Eupelmus vuilleti (7), and the honey bee, Apis mellifera (8), contain few interspersed repeated sequences corresponding to transposable elements. This might be due to the elimination of deleterious transposable elements in haploid males. However, R1 and R2 Line 1-like retrotransposable elements, which both have a specific insertion site in the 28S rRNA-encoding genes, are present in the 24 hymenopteran species tested to date (9, 10). These elements probably have no deleterious effects on insect viability because of the repeated structure of the 28S rRNA genes and therefore would not be eliminated by male haploidy. This insertion-site specificity may explain why they are found in Hymenoptera.Another transposon, mariner, has recently been shown to be widespread among insect orders (11). The complete copies of mariner-like elements (MLEs) in Drosophila mauritiana (Diptera) (12), Hyalophora cecropia (Lepidoptera) (13), and Chrysoptra plorabunda (Neuroptera) (14)

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Section: _ X ĩ G a C C G A T A C A A C A M A -R C C A C A T T G A C Cmentioning

confidence: 75%

“…The genomes of two parasitoid wasps, Diadromus pulchellus and Eupelmus vuilleti (7), and the honey bee, Apis mellifera (8), contain few interspersed repeated sequences corresponding to transposable elements. This might be due to the elimination of deleterious transposable elements in haploid males.…”

mentioning

confidence: 99%

Mariner-like elements in hymenopteran species: insertion site and distribution.

Bigot¹,

Hamelin²,

Capy³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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“…aegypti genome is organized in a ''short period interspersion pattern'' in which the single-copy DNAs are partitioned into small blocks by repetitive elements (40,47). In contrast, the majority of the D. melanogaster genome is organized in a ''long period interspersion pattern'' (43,48) in which single-copy DNAs are less interrupted by the interspersion of long repetitive elements. The presence of highly repetitive MITE-Like elements in Ae.…”

Section: Mite-like Elements and The Size And Organization Of Eukaryotmentioning

confidence: 98%

Three novel families of miniature inverted-repeat transposable elements are associated with genes of the yellow fever mosquito,Aedes aegypti

1997

Proc. Natl. Acad. Sci. U.S.A.

101

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Three novel families of transposable elements, Wukong, Wujin, and Wuneng, are described in the yellow fever mosquito, Aedes aegypti. Their copy numbers range from 2,100 to 3,000 per haploid genome. There are high degrees of sequence similarity within each family, and many structural but not sequence similarities between families. The common structural characteristics include small size, no coding potential, terminal inverted repeats, potential to form a stable secondary structure, A؉T richness, and putative 2-to 4-bp A؉T-biased specific target sites. Evidence of previous mobility is presented for the Wukong elements. Elements of these three families are associated with 7 of 16 fully or partially sequenced Ae. aegypti genes. Characteristics of these mosquito elements indicate strong similarities to the miniature inverted-repeat transposable elements (MITEs) recently found to be associated with plant genes. MITE-like elements have also been reported in two species of Xenopus and in Homo sapiens. This characterization of multiple families of highly repetitive MITE-like elements in an invertebrate extends the range of these elements in eukaryotic genomes. A hypothesis is presented relating genome size and organization to the presence of highly reiterated MITE families. The association of MITElike elements with Ae. aegypti genes shows the same bias toward noncoding regions as in plants. This association has potentially important implications for the evolution of gene regulation.

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“…bryos and larvae. Dr Ken Jones (University of Edinburgh) kindly provided a sample of DNA isolated as in [20]. This DNA had been purified by isopycnic centrifugation on CsCl gradients [21].…”

Section: Preparation Of Divamentioning

confidence: 99%

Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA

1983

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We have reported that production and characterization of antibodies highly specific to 5-methyl-cytosine (SmC) and the development of a sensitive immunochemicai method for the detection of 5mC in DNA [EBBS Lett. (1982) 150, 4691. Extension of this method to two other modified bases, 6-m~hyladenine (6mA) and %methyiguanine (7mG), is reported here. By use of this immunochemical approach, we are able to detect 5mC, 6mA and 7mG in human and Drosophila DNA and confirm their presence in the DNA of two mealybug species. DNA methylation Biotin-avidin cross-linking DNA-protein interaction Z-DNA X-chromosome inactivationGene regulation

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Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster

Cited by 94 publications

References 30 publications

Mariner-like elements in hymenopteran species: insertion site and distribution.

Mariner-like elements in hymenopteran species: insertion site and distribution.

Three novel families of miniature inverted-repeat transposable elements are associated with genes of the yellow fever mosquito,Aedes aegypti

Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA

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