Absence of siderophore activity in Legionella species grown in iron-deficient media

Abstract

Growth of Legionella species in a defined medium deficient in iron did not result in the production of phenolic or hydroxamate siderophores which could be detected by chemical or biological assay methods. Growth of a variety of other gram-negative organisms under the same conditions resulted in the production of both hydroxamate and phenolate siderophores. The iron-deficient medium limited growth of the Legionella species more severely than it did the growth of the other gram-negative organisms. We have concluded that Legionella species do not make the commonly recognized siderophores, probably because they are restricted in their growth to those environments in which inorganic iron is readily available or is supplied in a form bound to an unknown carrier.

Characterisation of antagonistic Bacillus and Pseudomonas strains for biocontrol potential and suppression of damping‐off and root rot diseases

“…The quantity of siderophore produced by bacterial isolates was estimated by the method described previously (Reeves et al , ). Bacteria were grown in King's B broth on a rotary shaker at 24 ± 2°C for 3 days and centrifuged at 12 100 g for 10 min and the supernatants were collected.…”

Cadmium‐induced changes in the production of siderophores by a plant growth promoting strain of Pseudomonas fulva

“…The coefficient of activity (W act ) reached nearly 10 and was one of the highest values we observed in the group of Pseudomonadales in our earlier studies [5]. Higher W act values (in the range of [30][31][32][33][34][35] were obtained only in the case of 5 strains of Streptomyces sp. isolated from the rhizosphere of Betula pendula L. and Alnus glutinosa L. growing at heavy metal contaminated sites in southern Poland [5].…”

Detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato

“…The quantity of siderophores produced by bacterial isolates was estimated by the method described previously (Reeves et al . ). The bacterial isolates were grown in King's B broth on a rotary shaker (150 rev min −1 ) at room temperature (24 ± 2°C) for 2 days.…”