Detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato

Zhang, L.; Khabbaz, Salah Eddin; Wang, A.; Li, H.; Abbasi, Pervaiz A.

doi:10.1111/jam.12728

Cited by 34 publications

(28 citation statements)

References 62 publications

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“…The split plate method, when the cultures were grown in liquid instead of solidified medium, led to a reduction in dry fungal biomass in comparison to the control plates. Zhang et al (2014) noted that BVCs were less effective overall than actual bacteria in inhibiting the growth of certain fungi including F. oxysporum . Our results suggested that contact was necessary for complete FOM- and FS-growth inhibition leading us to test whether CF extracts were involved in mycelial growth inhibition, which they were.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Bacillus Species Against Fusarium and Analysis of the Potential Mechanisms Used in Biocontrol

et al. 2018

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Fusarium is a complex genus of ascomycete fungi that consists of plant pathogens of agricultural relevance. Controlling Fusarium infection in crops that leads to substantial yield losses is challenging. These economic losses along with environmental and human health concerns over the usage of chemicals in attaining disease control are shifting focus toward the use of biocontrol agents for effective control of phytopathogenic Fusarium spp. In the present study, an analysis of the plant-growth promoting (PGP) and biocontrol attributes of four bacilli (Bacillus simplex 30N-5, B. simplex 11, B. simplex 237, and B. subtilis 30VD-1) has been conducted. The production of cellulase, xylanase, pectinase, and chitinase in functional assays was studied, followed by in silico gene analysis of the PGP-related and biocontrol-associated genes. Of all the bacilli included in this study, B. subtilis 30VD-1 (30VD-1) demonstrated the most effective antagonism against Fusarium spp. under in vitro conditions. Additionally, 100 μg/ml of the crude 1-butanol extract of 30VD-1’s cell-free culture filtrate caused about 40% inhibition in radial growth of Fusarium spp. Pea seed bacterization with 30VD-1 led to considerable reduction in wilt severity in plants with about 35% increase in dry plant biomass over uninoculated plants growing in Fusarium-infested soil. Phase contrast microscopy demonstrated distortions and abnormal swellings in F. oxysporum hyphae on co-culturing with 30VD-1. The results suggest a multivariate mode of antagonism of 30VD-1 against phytopathogenic Fusarium spp., by producing chitinase, volatiles, and other antifungal molecules, the characterization of which is underway.

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Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Bacillus Species Against Fusarium and Analysis of the Potential Mechanisms Used in Biocontrol

et al. 2018

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“…The results of the present study suggest that fluorescent pseudomonads can have an antagonistic effect that is not necessarily associated with the antibiotics that were searched for here. Several reports on plant-associated pseudomonads indicated also a role of siderophores, lytic enzymes, hydrogen cyanide and ammonia as well as organic volatiles in the inhibition of fungal phytopathogens ( Alimi et al, 2012 ; Loper et al, 2012 ; Müller et al, 2013 ; Zhang et al, 2014 ; De Vrieze et al, 2015 ; Yasmin et al, 2016 ). Wagner et al (2018) most recently examined Pseudomonas strains to identify the gene clusters that are responsible for antagonism against oomycetes by transposon mutagenesis combined with high throughput sequencing and antagonism tests.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Potential of Fluorescent Pseudomonads Colonizing Wheat Heads Against Mycotoxin Producing Alternaria and Fusaria

et al. 2018

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Natural control of phytopathogenic microorganisms is assumed as a priority function of the commensal plant microbiota. In this study, the suitability of fluorescent pseudomonads in the phyllosphere of crop plants as natural control agents was evaluated. Under field conditions, ears of winter wheat were found to be colonized with high consistency and at a high density by pseudomonads at the late milk dough stage. Isolates of these bacteria were evaluated for their potential to protect the plants from phytopathogenic Alternaria and Fusarium fungi. More Pseudomonas isolates were antagonistically active against alternaria than against fusaria in the dual culture test. The alternaria responded species-specifically and more sensitively to bacterial antagonism than the strain-specific reacting fusaria. A total of 110 randomly selected Pseudomonas isolates were screened for genes involved in the biosynthesis of the antibiotics 2,4-diacetylphloroglucinol, phenazine-1-carboxylic acid, pyoluteorin, and pyrrolnitrin. The key gene for production of the phloroglucinol was found in none of these isolates. At least one of the genes, encoding the biosynthesis of the other antibiotics was detected in 81% of the isolates tested. However, the antagonistic effect found in the dual culture assay was not necessarily associated with the presence of these antibiotic genes. Wheat grains as natural substrate were inoculated with selected antagonistic Pseudomonas isolates and Alternaria and Fusarium strains, respectively. The fungal growth was only slightly delayed, but the mycotoxin production was significantly reduced in most of these approaches. In conclusion, the distribution of phytopathogenic fungi of the genera Alternaria and Fusarium in the field is unlikely to be inhibited by naturally occurring pseudomonads, also because the bacterial antagonists were not evenly distributed in the field. However, pseudomonads can reduce the production of Alternaria and Fusarium mycotoxins in wheat grains and thus have the potential to improve the crop quality.

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“…GC/MS analysis of headspace extracts from this bacterium revealed the release of 21 compounds (S1 Table), among them sulfur-containing volatiles such as methanethiole, dimethyl disulfide ( 1 ), dimethyl trisulfide ( 2 ), S -methyl ethanethioate ( 3 ), S -methyl propanethioate ( 4 ), S -methyl 2-methylpropanethioate ( 5 ), S -methyl 3-methylbutanethioate ( 6 ) and S -methyl phenylethanethioate ( 7 ), as well as γ-decalactone ( 8 ) (S3 Fig). Some these compounds, for example, dimethyl trisulfide and S -methyl 3-methylbutanethioate, have been associated with inhibition of a variety of plant pathogens [52,53], suggesting they may play a role in suppression of amphibian fungal pathogens, such as Bd and Bsal .…”

Section: Resultsmentioning

confidence: 99%

Amphibian skin-associated Pigmentiphaga: Genome sequence and occurrence across geography and hosts

et al. 2019

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The bacterial communities colonizing amphibian skin have been intensively studied due to their interactions with pathogenic chytrid fungi that are causing drastic amphibian population declines. Bacteria of the family Alcaligenaceae, and more specifically of the genus Pigmentiphaga, have been found to be associated specifically to arboreal frogs. Here we analyze their occurrence in a previously assembled global skin microbiome dataset from 205 amphibian species. Pigmentiphaga made up about 5% of the total number of reads in this global dataset. They were mostly found in unrelated arboreal frogs from Madagascar (Mantellidae and Hyperoliidae), but also occurred at low abundances on Neotropical frogs. Based on their 16S sequences, most of the sequences belong to a clade within Pigmentiphaga not assignable to any type strains of the five described species of the genus. One isolate from Madagascar clustered with Pigmentiphaga aceris (>99% sequence similarity on 16S rRNA gene level). Here, we report the full genome sequence of this bacterium which, based on 16S sequences of >97% similarity, has previously been found on human skin, floral nectar, tree sap, stream sediment and soil. Its genome consists of a single circular chromosome with 6,165,255 bp, 5,300 predicted coding sequences, 57 tRNA genes, and three rRNA operons. In comparison with other known Pigmentiphaga genomes it encodes a higher number of genes associated with environmental information processing and cellular processes. Furthermore, it has a biosynthetic gene cluster for a nonribosomal peptide syntethase, and bacteriocin biosynthetic genes can be found, but clusters for β-lactones present in other comparative Pigmentiphaga genomes are lacking.

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Detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato

Abstract: Indigenous antagonistic bacterial isolates have the potential to be developed as biofungicides for minimizing early crop losses due to soilborne diseases caused by Fusarium and other soilborne pathogens.

Cited by 34 publications

References 62 publications

Antifungal Activity of Bacillus Species Against Fusarium and Analysis of the Potential Mechanisms Used in Biocontrol

Antifungal Activity of Bacillus Species Against Fusarium and Analysis of the Potential Mechanisms Used in Biocontrol

Antagonistic Potential of Fluorescent Pseudomonads Colonizing Wheat Heads Against Mycotoxin Producing Alternaria and Fusaria

Amphibian skin-associated Pigmentiphaga: Genome sequence and occurrence across geography and hosts

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