Absence of the<i>mec</i>A Gene in Methicillin Resistant<i>Staphylococcus aureus</i>Isolated from Different Clinical Specimens in Shendi City, Sudan

Elhassan, Mogahid M.; Ozbak, Hani A.; Hemeg, Hassan A.; Elmekki, Miskelyemen A.; Ahmed, Leila M.

doi:10.1155/2015/895860

Cited by 76 publications

(65 citation statements)

References 23 publications

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“…In agreement with our results, the absence of mecA gene within resistant staphylococcal isolates was reported worldwide [38–40]. For example, Elhassan et al reported twelve out of the 123 MRSA isolates (9.8%) were mecA negative when using PCR targeting mecA gene [41]. Inconsistency between phenotypic and genotypic findings may result from mutations in the primers binding sites within the gene.…”

Section: Discussionsupporting

confidence: 90%

Nasal carriage of methicillin resistant Staphylococcus aureus among health care workers at Al Shifa hospital in Gaza Strip

Aila

Laham²,

Ayesh

2017

BMC Infect Dis

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BackgroundNasal carriage of Staphylococcus aureus among hospital personnel is a common cause of hospital acquired infections. Emergence of drug resistant strains especially methicillin resistant S. aureus (MRSA) is a serious problem in hospital environment. Therefore, the aim of this study was to determine the nasal carriage rate of S. aureus and MRSA among Health Care Workers (HCWs) at Al Shifa Hospital, the major hospital in Gaza Strip.MethodsA cross sectional study was conducted on 200 HCWs. Nasal swabs were collected during February — April 2015, and cultured on blood and mannitol salt agar. The isolates were identified as S. aureus based on morphology, coagulase test, DNase test and mannitol salt agar fermentation. Disk diffusion antibiotic susceptibility tests were performed according to the guidelines of the Clinical and Laboratory Standards Institute. MRSA were confirmed by detection of the mecA gene by PCR.ResultsOut of the 200 healthcare workers, 62 (31%) carried S. aureus, of which 51 (82.3%) were MRSA. Therefore, 25.5% of all HCWs were identified as MRSA carriers. MRSA carriage rate was highest among nurses (30.4%) whereas the carriage rate among doctors was (16%). The majority of MRSA carriers were workers of internal medicine department and surgical wards (41.3 and 35% respectively). Out of the 51 MRSA isolates identified by oxacillin disc resistance, 40 were confirmed by PCR targeting the mecA gene. Penicillin showed the highest rate of resistance among MRSA and MSSA isolates (100%).ConclusionsThe high rate of nasal MRSA carriage among healthcare workers found in this study is alarming and highlights the need for adjusted infection control measures to prevent MRSA transmission from HCWs to the vulnerable patient.

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Section: Discussionsupporting

confidence: 90%

Nasal carriage of methicillin resistant Staphylococcus aureus among health care workers at Al Shifa hospital in Gaza Strip

Aila

Laham²,

Ayesh

2017

BMC Infect Dis

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“…Here, resistance to oxacillin by disk diffusion was detected in 13 of 44 strains (29,55%). However, PCR analysis for mecA revealed that seven out of the 13 strains classified as resistant to oxacillin by disk diffusion harbored the mecA gene and five strains that were positive for mecA (ELHASSAN et al, 2015;BOURGUIGNON et al, 2016). Although, mecA seems to be the most common gene associated with meticillin resistance in S. pseudintermedius, some studies have shown that another gene (mecC) could also be responsible for this resistance profile (KJELLMAN et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa

et al. 2018

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Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis. Swabs from both ears of 52 dogs with otitis (n=104) were included. Bacteria were cultured using Muller-Hinton agar (supplemented with 5% equine blood and incubated at 37°C for 24 to 48 hours. All colonies underwent biochemical evaluation for identification of staphylococci. The identity of colonies as SP was confirmed by polymerase chain reaction. The antimicrobial susceptibility of SP strains was evaluated by disk diffusion. The presence of the gene mecA was evaluated in all SP isolates by PCR. Forty-four SP strains were isolated from swabs of 31 dogs (31/52, 59.6%). Seventy-five percent of the strains were susceptible to cephalexin and 93.2% to amoxicillin plus clavulanic acid. Less than 23% of the strains were susceptible to penicillin. For non-beta-lactam antimicrobials, 63.6% of the strains showed resistance to sulfamethoxazole-trimethoprim, 61.4% to tetracycline, and 38, 64% to enrofloxacin. Aminoglycoside resistance rate was 27.3% for gentamicin. Resistance to oxacillin in vitro was detected in 13 of the 44 strains (29, 55%). A total of 12 strains (27.3%) were positive for mecA gene and five of these 12 strains were susceptible to in vitro oxacillin. Twenty-six (59, 1%) strains were resistant to three or more classes of antimicrobials, and classified as multi resistant. Our results showed high frequency of SP and multi resistant isolates to antimicrobials commonly used in veterinary.

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“…However, a new mecA homologue mecALGA251 shared 70% nucleotide homology with mecA [41], which results in possible false negative results by using this method. Meanwhile, the detection of the mecA gene is not specific to MRSA, because a small proportion of methicillin-susceptible Staphylococcus aureus (MSSA) with mecA gene and a large proportion of MRSA strains lack of mecA [42,43]. Combination CRISPR-Cas9 with optical DNA mapping was also applied to identify bacterial antibiotic resistance genes [39].…”

Section: Type Ii: Crispr-cas9 Based Diagnosticsmentioning

confidence: 99%

CRISPR-cas systems based molecular diagnostic tool for infectious diseases and emerging 2019 novel coronavirus (COVID-19) pneumonia

Xiang

Qian

Zhang

et al. 2020

Journal of Drug Targeting

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Emerging infectious diseases, the persistent potential for destabilising pandemics, remain a global threat leading to excessive morbidity and mortality. The current outbreak of pneumonia caused by 2019 novel coronavirus (COVID-19) illustrated difficulties in lack of effective drugs for treatment. Accurate and rapid diagnostic tools are essential for early recognition and treatment of infectious diseases, allowing timely implementation of infection control, improved clinical care and other public health measures to stop the spread of the disease. CRISPR-Cas technology speed up the development of infectious disease diagnostics with high rapid and accurate. In this review, we summarise current advance regarding diverse CRISPR-Cas systems, including CRISPR-Cas9, CRISPR-Cas12 and CRISPR-Cas13, in the development of fast, accurate and portable diagnostic tests and highlight the potential of CRISPR-Cas13 in COVID-19 Pneumonia and other emerging infectious diseases diagnosis. ARTICLE HISTORY

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Absence of themecA Gene in Methicillin ResistantStaphylococcus aureusIsolated from Different Clinical Specimens in Shendi City, Sudan

Cited by 76 publications

References 23 publications

Nasal carriage of methicillin resistant Staphylococcus aureus among health care workers at Al Shifa hospital in Gaza Strip

Nasal carriage of methicillin resistant Staphylococcus aureus among health care workers at Al Shifa hospital in Gaza Strip

Frequency and antimicrobial susceptibility of Staphylococcus pseudintermedius in dogs with otitis externa

CRISPR-cas systems based molecular diagnostic tool for infectious diseases and emerging 2019 novel coronavirus (COVID-19) pneumonia

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