Absolute and Relative Quantification of Multiplex DNA Assays Based on an Elemental Labeling Strategy

ICP-MS DNA assay based on lanthanide labels and hybridization chain reaction amplification

“…On the other hand, smears appeared in the 5th, 6th and 7th lanes when different concentrations of capture DNA probe 2 were mixed with H1 and H2-Ln, indicating that HCR had occurred and formed long-nicked DNA polymers in the presence of the initiation sequence. 19 The detection limit and range are comparable with previously reported rolling circle amplication (RCA) and ligase chain reaction based ICP-MS DNA assay. Therefore, the resulting polymers in lanes 5, 6, and 7 appear as smears instead of bands, which is consistent with other research involved with HCR amplication.…”

“…Conventionally well-established techniques for nucleic acid assay such as northern blotting technology 1 and DNA microarrays 2 without amplication steps might hinder their applications in nucleic acid clinical diagnostics. 19 This work indicates the excellent ability of the ICP-MS technique for high-level multiplexing analysis. [3][4][5][6][7][8][9] The uorescent labeling approach has been widely used in these methods for signal readout due to its sensitivity and easy labeling process.…”

Inductively coupled plasma mass spectrometry‐based immunoassay: A review

“…This technique gave a new way for efficient and accurate label free multiplex protease assay. Absolute and relative quantification of multiple DNAs based on elemental labeling was accomplished by Zhang et al (Han et al, ) Rare‐earth elements, indium, and stable isotopes macrocyclic compounds were labeled to oligonucleotides serving as DNA probes. Fifteen clinical diseases (cancer, heredopathia, and virus) associated DNA targets were simultaneously detected.…”

Cerium oxide-deposited mesoporous silica nanoparticles for the determination of carcinoembryonic antigen in serum using inductively coupled plasma-mass spectrometry

“…However, since a biomarker can be expressed by various cancers, and alternatively, a cancer may contain various biomarkers, multiplex detection is inevitable for improving diagnostic accuracy and speed in clinical application. In this concept, element tagging demonstrated excellent analytical performance for multiplex detection of biomarkers using various rare earth elements [1][2][3][4]. Recently, nanoparticle tagging instead of element tagging demonstrated a significantly improved detection limit owing to signal amplification [5].…”