Absolute CD4<sup>+</sup> cell count using a plastic microchip and a microscopic cell counter

Bae, Sun Hyun; Park, Hae Chul; Oh, Jun Seo; Yoon, Soo Young; Park, Dae Won; Choi, In Keun; Kim, Hae-Joong; Oh, Jung Hyun; Hur, Dae Sung; Chung, Chong-Pyoung; Chang, Jun Keun; Robinson, J. Paul; Lim, Chae Seung

doi:10.1002/cyto.b.20470

Cited by 9 publications

(9 citation statements)

References 22 publications

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“…A recent study showed similar performance to flow cytometry in an HIV infected cohort [27]. Furthermore, a separate study showed that T cells can be distinguished from monocytes based on their different intensity after staining with fluorescent anti-hCD4 [28]. The CD4 counts from this microchannel method correlate closely with results of flow cytometry in 100 specimens, but blood dilution and staining are yet to be automated.…”

Section: The Need For Point Of Care Technologiesmentioning

confidence: 91%

“…In addition to being single platform and potentially portable, imaging cytometry detects a large number of cells simultaneously, while FACS measures one cell at a time. Of the several imaging systems described in this section above, detection of the sandwich structures has a higher accuracy by eliminating monocyte interference [25,26,29,33], while label-free imaging of all cells captured by a single antibody [28,34–37] offers greater operation simplicity and faster turn-around time. The field performances of these systems are yet to be established to determine if the strength of each system outweighs its shortcomings.…”

Section: The Need For Point Of Care Technologiesmentioning

confidence: 99%

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Emerging technologies for point-of-care CD4 T-lymphocyte counting

Boyle¹,

Hawkins²,

Steele³

et al. 2012

Trends in Biotechnology

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A CD4 T-lymphocyte count determines eligibility for antiretroviral therapy (ART) with patients recently diagnosed with HIV and also monitors the efficacy of ART treatment thereafter. ART slows the progression of HIV to AIDS. In the developing world, CD4 tests are often performed in centralized laboratories, typically in urban areas. The expansion of ART programs into rural areas has created a need for rapid CD4 counting as logistical barriers can delay the timely dissemination of test results and affect patient care through delay in intervention or loss of follow-up care. CD4 measurement at the point-of-care (POC) in rural areas could help facilitating ART and monitoring of treatment. This review highlights recent technology developments with applications towards determining CD4 counts at the POC.

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Section: The Need For Point Of Care Technologiesmentioning

confidence: 91%

Section: The Need For Point Of Care Technologiesmentioning

confidence: 99%

Emerging technologies for point-of-care CD4 T-lymphocyte counting

Boyle¹,

Hawkins²,

Steele³

et al. 2012

Trends in Biotechnology

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show abstract

“…Some designs relied on the even distribution of cells in a plastic chamber to produce accurate counts [80]. Others have used a microfabricated membrane to filter out erythrocytes, leaving leukocytes, which were fluorescently labeled [81].…”

Section: B Global Health Solutions 1) Optical Methodsmentioning

confidence: 99%

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

Damhorst

Watkins

Bashir

2013

IEEE Trans. Biomed. Eng.

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“…The new cell viability test was developed by modifying (or improving) the previously reported microscopic technique for counting residual WBC with microchannel chip 3, 4. The microchannel chip was made with polymethylmetacrylate (PMMA).…”

Section: Methodsmentioning

confidence: 99%

Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods

Kim¹,

Nam²,

An³

et al. 2011

Clinical Laboratory Analysis

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The cell viability test is an essential tool in any laboratory, performing cell-based studies and clinical laboratory tests. The trypan blue exclusion method is the most popular assay for its simple concept among various diagnostic tools. However, several disadvantages include time-consuming and labor-intensive steps with low precision. In this study, we evaluated a new technique for the automatic cell viability measurement with microscopic cell counter and microchip. Upon blood draw from 11 healthy volunteers, Mononuclear cells were separated immediately from the heparinized whole blood, and the viable cells were diluted from 100 to 1%. The cell viability tests were performed simultaneously with following three methods: the conventional manual trypan blue exclusion method; the flow cytometry measurement with propidium iodide stain; and the newly developed microscopic cell counter with microchip. Linearities, precisions, and correlations from three methods were analyzed and compared. The correlations data from the microscopic cell counter were in good agreement with both the conventional trypan blue method (r=0.99, P<0.05) and the flow cytometry (r=0.99, P<0.05), respectively. The precision (2.0-6.2%) and linearity from the microscopic cell counter method with microchip were superior in comparison with the conventional method. The microscopic cell counter with microchip performed well with high precision, linearity, and efficient running time than both the manual trypan blue and the flow cytometry methods.

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Absolute CD4⁺ cell count using a plastic microchip and a microscopic cell counter

Abstract: We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD41 T-cells with the use of plastic microchips. This new system is an adaptation of a ''nolyse, no-wash,'' volumetric single platform assay, and absolute CD4

Cited by 9 publications

References 22 publications

Emerging technologies for point-of-care CD4 T-lymphocyte counting

Emerging technologies for point-of-care CD4 T-lymphocyte counting

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods

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Absolute CD4+ cell count using a plastic microchip and a microscopic cell counter

Abstract: We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD41 T-cells with the use of plastic microchips. This new system is an adaptation of a ''nolyse, no-wash,'' volumetric single platform assay, and absolute CD4

Cited by 9 publications

References 22 publications

Emerging technologies for point-of-care CD4 T-lymphocyte counting

Emerging technologies for point-of-care CD4 T-lymphocyte counting

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods

Contact Info

Product

Resources

About

Absolute CD4⁺ cell count using a plastic microchip and a microscopic cell counter