Absolute measurements and certified reference material for iron isotopes using multiple‐collector inductively coupled mass spectrometry

Zhou, Tao; Zhao, Mo-Tian; Wang, Jun; Lu, Hai

doi:10.1002/rcm.3406

Cited by 10 publications

(9 citation statements)

References 6 publications

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“…All Fe isotopes were simultaneously detected together with 60 Ni as a monitor for the correction of isobaric interferences of 58 Ni on 58 Fe in one sequence. A mixture of argon and H 2 were used as collision gas to eliminate the interferences [ 21 ]. Under optimized conditions, external precision of the order of 0.01–0.03% (relative standard deviation, RSD), and 0.1–0.3% (RSD) was obtained for 54 Fe/ 56 Fe, 57 Fe/ 56 Fe and 58 Fe/ 56 Fe, respectively.…”

Section: Methodsmentioning

confidence: 99%

Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique

Cai

Zhang²,

Wang³

et al. 2018

Nutr Metab (Lond)

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BackgroundIron is a kind of essential trace mineral in the human body, while the studies on its physiological requirement are very limited recently, especially in China. And most studies were performed with the radioisotope tracer technique, which was harmful to health. This study aimed to first get the value of iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique.MethodsForty-four eligible young Chinese healthy adults were randomly recruited from the Bethune Military Medical College (Shijiazhuang, Hebei, China) between January 2010 and March 2011, and 19 subjects were included in the final data analysis. After adaptive diets and observation, subjects received 58Fe intravenously. The baseline venous blood sample and general information were collected on day 0. Venous blood samples were also collected on day 14, 30, 60, 100, 120, 150, 240, 330, 425, 515, 605, 767, 1155, respectively. The blood samples were acid digested by a Microwave Digestion System and then analyzed by the MC-ICP-MS and Atomic Absorption Spectroscopy to get the abundance of Fe isotopes and the total iron concentration respectively. The circulation rate (the proportion of blood iron to whole body iron) could be calculated by the intake amount, background content and the peak isotope content. When the abundance changed stably, the iron physiological requirement could be calculated by the iron loss in a period of time.ResultsThe abundance of 58Fe reached its peak on day 14, and changed stably from day 425. The average circulation rate was 84%, with no significance difference between the 2 genders. The mean iron requirement in females was 1101.68 μg/d, and the mean requirement adjusted by body weight was 20.69 μg/kg.d. For males, the mean iron requirement was 959.9 μg/d, and the requirement adjusted by body weight was 14.04 μg/kg.d.ConclusionOur study has obtained the data about the iron physiological requirements of Chinese adults using stable isotope labeling technique, which could provide the basis for adjusting iron DRIs of Chinese people in the future.Trial registrationThe trial was registered at the Chinese Clinical Trial Registry (No: ChiCTR-TRC-09000581).

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Section: Methodsmentioning

confidence: 99%

Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique

Cai

Zhang²,

Wang³

et al. 2018

Nutr Metab (Lond)

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“…All Fe isotopes were detected simultaneously in one sequence together with 60 Ni as monitor for the correction of isobaric interferences of 58 Ni on 58 Fe. Mixture of Ar and H 2 were used as collision gas to eliminate the interferences [ 37 ]. Under the optimized conditions, external precisions on the order of 0.01–0.03% (relative standard deviation, RSD), and 0.1–0.3% (RSD) were obtained for 57 Fe/ 56 Fe and 58 Fe/ 56 Fe, respectively.…”

Section: Methodsmentioning

confidence: 99%

Non-Heme Iron Absorption and Utilization from Typical Whole Chinese Diets in Young Chinese Urban Men Measured by a Double-Labeled Stable Isotope Technique

Yang

Zhang²,

Wang

et al. 2016

PLoS ONE

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BackgroundThis study was to observe the non-heme iron absorption and biological utilization from typical whole Chinese diets in young Chinese healthy urban men, and to observe if the iron absorption and utilization could be affected by the staple food patterns of Southern and Northern China.Materials and MethodsTwenty-two young urban men aged 18–24 years were recruited and randomly assigned to two groups in which the staple food was rice and steamed buns, respectively. Each subject received 3 meals containing approximately 3.25 mg stable 57FeSO4 (the ratio of 57Fe content in breakfast, lunch and dinner was 1:2:2) daily for 2 consecutive days. In addition, approximately 2.4 mg 58FeSO4 was administered intravenously to each subject at 30–60 min after dinner each day. Blood samples were collected from each subject to measure the enrichment of the 57Fe and 58Fe. Fourteen days after the experimental diet, non-heme iron absorption was assessed by measuring 57Fe incorporation into red blood cells, and absorbed iron utilization was determined according to the red blood cell incorporation of intravenously infused 58Fe SO4.ResultsNon-heme iron intake values overall, and in the rice and steamed buns groups were 12.8 ±2.1, 11.3±1.3 and 14.3±1.5 mg, respectively; the mean 57Fe absorption rates were 11±7%, 13±7%, and 8±4%, respectively; and the mean infused 58Fe utilization rates were 85±8%, 84±6%, and 85±10%, respectively. There was no significantly difference in the iron intakes, and 57Fe absorption and infused 58Fe utilization rates between rice and steamed buns groups (all P>0.05).ConclusionWe present the non-heme iron absorption and utilization rates from typical whole Chinese diets among young Chinese healthy urban men, which was not affected by the representative staple food patterns of Southern and Northern China. This study will provide a basis for the setting of Chinese iron DRIs.

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“…This is achieved by gravimetrically blending high-purity isotopically enriched materials and obtaining the mass bias correction factors through comparison of instrumental results with predetermined blend ratios, and using these to correct ratios in samples. This approach was recently used for Fe (Zhou et al, 2008), Zn (Ponzevera et al, 2006), Cd (Pritzkow et al, 2007), and Nd (Zhao et al, 2005) systems. This strategy suffers drawbacks similar to those of the normal SSB method.…”

Section: External Standard-sample-standard Bracketing (Ssb) Technimentioning

confidence: 99%

Accurate and precise determination of isotopic ratios by MC‐ICP‐MS: A review

Yang

2009

Mass Spectrometry Reviews

240

222

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For many decades the accurate and precise determination of isotope ratios has remained a very strong interest to many researchers due to its important applications in earth, environmental, biological, archeological, and medical sciences. Traditionally, thermal ionization mass spectrometry (TIMS) has been the technique of choice for achieving the highest accuracy and precision. However, recent developments in multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS) have brought a new dimension to this field. In addition to its simple and robust sample introduction, high sample throughput, and high mass resolution, the flat-topped peaks generated by this technique provide for accurate and precise determination of isotope ratios with precision reaching 0.001%, comparable to that achieved with TIMS. These features, in combination with the ability of the ICP source to ionize nearly all elements in the periodic table, have resulted in an increased use of MC-ICP-MS for such measurements in various sample matrices. To determine accurate and precise isotope ratios with MC-ICP-MS, utmost care must be exercised during sample preparation, optimization of the instrument, and mass bias corrections. Unfortunately, there are inconsistencies and errors evident in many MC-ICP-MS publications, including errors in mass bias correction models. This review examines "state-of-the-art" methodologies presented in the literature for achievement of precise and accurate determinations of isotope ratios by MC-ICP-MS. Some general rules for such accurate and precise measurements are suggested, and calculations of combined uncertainty of the data using a few common mass bias correction models are outlined.

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Absolute measurements and certified reference material for iron isotopes using multiple‐collector inductively coupled mass spectrometry

Cited by 10 publications

References 6 publications

Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique

Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique

Non-Heme Iron Absorption and Utilization from Typical Whole Chinese Diets in Young Chinese Urban Men Measured by a Double-Labeled Stable Isotope Technique

Accurate and precise determination of isotopic ratios by MC‐ICP‐MS: A review

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