Absolute molecular weight determination of hypromellose acetate succinate by size exclusion chromatography: Use of a multi angle laser light scattering detector and a mixed solvent

Chen, Raymond; Ilasi, Nicholas; Sekulic, Sonja

doi:10.1016/j.jpba.2011.07.035

Cited by 19 publications

(16 citation statements)

References 12 publications

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“…Briefly, 1 g HME extrudate was weighed and then dissolved in the mobile phase in a 10‐ml volumetric flask via sonication for 10 min (Chen, Ilasi, & Sekulic, ). The mobile phase consisted of acetonitrile: water (25:75, v/v) (Skibic et al., ).…”

Section: Methodsmentioning

confidence: 99%

Preparation, characterization, and pharmacokinetics in swine of a florfenicol enteric formulation prepared using hot‐melt extrusion technology

Wen

Feng

et al. 2018

Vet Pharm & Therapeutics

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The objective of this work was to manufacture an enteric formulation of florfenicol (FF) using hot-melt extrusion (HME) technology and to evaluate its in vitro dissolution and in vivo pharmacokinetics. For the HME process, hypromellose acetate succinate LG (HPMCAS-LG) was the enteric polymer mixed with FF, and the two components were extruded with a standard screw configuration at a speed of 50 rpm. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), powder X-ray diffraction (PXRD), and Fourier transform infrared spectroscopy (FT-IR) were performed to characterize the HME extrudate. The release percentage of the enteric formulation in the acidic stage was <10% of the loaded FF, whereas that in the phosphate buffer stage was >80%. Pharmacokinetic evaluations in swine revealed that the enteric formulation had a longer t and MRT than commercially available FF powder (FULAIKA ), indicating that the novel formulation exhibited enteric and sustained release properties. Compared with the commercial product, the relative bioavailability of the enteric formulation reached up to 117.2%. This study suggests that this formulation may have potential for future commercialization.

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Section: Methodsmentioning

confidence: 99%

Preparation, characterization, and pharmacokinetics in swine of a florfenicol enteric formulation prepared using hot‐melt extrusion technology

Wen

Feng

et al. 2018

Vet Pharm & Therapeutics

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show abstract

“…6). The latter fact indicates that very low concen trations of "species" are eluted from the column [21], which may come from the partial adsorption of SSMA due to hydrophobic interactions with the stationary phase. The intense MALLS signal at high M w values is probably dealt with strong SSMA aggregation.…”

Section: Resultsmentioning

confidence: 99%

“…The solution was allowed to dissolve at room temperature in the capped vial for 24 h with gentle shaking occasionally. The normaliza tion solution and sample solution were filtered into HPLC vials through a syringe filter respectively [21].…”

Section: Introductionmentioning

confidence: 99%

Absolute molecular mass determination of sodium salt of styrene/methacrylic acid copolymer by gel permeation chromatography coupled with multi angle laser light scattering

et al. 2015

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Sodium salt of styrene/methacrylic acid copolymer was synthesized via radical polymerization, and its absolute molecular mass and polydispersity index were measured by aqueous based GPC coupled with MALLS in a mixed solvent containing 75/25, v/v%, methanol/water, 38.6 mM NaCl and 10.0 mM NaH 2 PO 4 buffer, pH 6.0. Dynamic light scattering verified the absence of aggregation of copolymer in pre ferred mobile phase compositions.

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“…With the development of MALLS technique, SEC-MALLS is widely accepted as a preferred method for determining polymer absolute molecular weight today (Chen, Ilasi, & Sekulic, 2011). Nonetheless, accurate determination of the M w of XG is difficult because of its very high molecular weight, the stiffness of the molecules and the presence of aggregates between chains (Born et al, 2005).…”

Section: Sec-malls Resultsmentioning

confidence: 99%

Development, validation and influence factor analysis of a near-infrared method for the molecular weight determination of xanthan gum

Song

Ling

Zang

et al. 2015

Carbohydrate Polymers

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Absolute molecular weight determination of hypromellose acetate succinate by size exclusion chromatography: Use of a multi angle laser light scattering detector and a mixed solvent

Cited by 19 publications

References 12 publications

Preparation, characterization, and pharmacokinetics in swine of a florfenicol enteric formulation prepared using hot‐melt extrusion technology

Preparation, characterization, and pharmacokinetics in swine of a florfenicol enteric formulation prepared using hot‐melt extrusion technology

Absolute molecular mass determination of sodium salt of styrene/methacrylic acid copolymer by gel permeation chromatography coupled with multi angle laser light scattering

Development, validation and influence factor analysis of a near-infrared method for the molecular weight determination of xanthan gum

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