Absorption and translocation of copper and arsenic in an aquatic macrophyte Myriophyllum alterniflorum DC. in oligotrophic and eutrophic conditions

Abstract

The aim of this study is to evaluate copper and arsenic accumulation and translocation at a concentration of 100 μg/L of a submersed macrophyte Myriophyllum alterniflorum. The trophic level (eutrophic and oligotrophic conditions) of the medium was also considered. To achieve this goal, plants were incubated for 21 days in the presence of 100 μg/L of Cu or AsV. The heavy metal transfers from the contaminated medium to plants and into plant tissues was discussed in terms of the bioconcentration factor (BCF) and the translocation factor (TF). Malondialdehyde (MDA) content in tissues was analyzed in order to study the toxicity of these two contaminants. Our results show that copper was more accumulated in shoots, than roots, whereas the opposite trend was observed for arsenic. In addition, the two contaminants were more accumulated in oligotrophic than eutrophic medium. The BCF of copper in shoots was 1356 in oligotrophic condition, while that of arsenic was higher in roots about 620 in the same condition. The TF was less than 1 for arsenic, and higher than 1 for copper, indicating that watermilfoil restrains the translocation of arsenic to shoots, while it has a low capacity to control the translocation of an essential micronutrient like copper. An increase in MDA content was observed under Cu and As stress. On the basis of this experiment, M. alterniflorum has a higher accumulation potential of copper and arsenic, and therefore, it can be a good candidate for the phytofiltration of these two contaminants from water.

Comparative in vitro/in situ approaches to three biomarker responses of Myriophyllum alterniflorum exposed to metal stress

“…Accordingly, such objectives require constantly evaluating water quality by means of biomonitoring. Fish, mollusks and invertebrates are extensively used as bioindicators of metal pollution (Van Praet et al, 2014;Wang and Lu, 2017;Bouzahouane et al, 2018;Souza et al, 2018), although aquatic plants are also increasingly being investigated for these ecological surveys (Ferrat et al, 2003;Krayem et al, 2016;Reale et al, 2016). More specifically, the analyses of association, composition and richness of plant species during in situ studies have become even more widespread and in depth.…”

“…in situ accumulation of cobalt, lead, zinc, nickel, iron and manganese in Myriophyllum aquaticum (Harguinteguy et al, 2013(Harguinteguy et al, , 2016 or in Myriophyllum spicatum (Galal and Shehata, 2014). Cu, Cd or arsenic hyperaccumulation in Myriophyllum alterniflorum has also been evaluated from in vitro cultures (Delmail et al, 2011b(Delmail et al, , 2011c2013;Krayem et al, 2016;Krayem et al, 2018). In these last studies however and in contrast with other studies, biological changes were also measured through biomarker response analyses focusing on scavengers indicating the oxidative stress generated by reactive oxygen species (ROS) and/or antioxidant systems.…”

Phytoremediation of Arsenic Contaminated Water Using Aquatic, Semi-Aquatic and Submerged Weeds

“…had been widely reported to accumulate As in their body parts from contaminated soils and water [16,17,[48][49][50][51]. Alternanthera philoxeroides had potential to extract As from contaminated water and stored in root system [52,53]. Reports from previous studies indicates that As accumulation in A. philoxeroides followed in the order of root > stem > leaf and average BCF for root ranged from 106 to 191, when exposed to various doses of As containing solutions (1, 2 and 5 mg kg −1 ) under laboratory condition [52].…”

“…Reports from previous studies indicates that As accumulation in A. philoxeroides followed in the order of root > stem > leaf and average BCF for root ranged from 106 to 191, when exposed to various doses of As containing solutions (1, 2 and 5 mg kg −1 ) under laboratory condition [52]. Under natural condition, Alternanthera philoxeroides observed to uptake 12.94 mg kg −1 total As dw from pulp paper industry water with average BCF-3.58 and TF-0.51 [53]. Higher BCF under laboratory condition observed due to used of higher As containing solution and availability of external nutrients for weed plants which may trigger As uptake through phosphate uptake pathway.…”

Are Myriophyllum alterniflorum biomarker responses to arsenic stress differentially affected by hydrodynamic conditions?

“…and or nutrient enrichment (Krayem et al, 2016a(Krayem et al, , 2016b. It has been demonstrated that water movement plays a key role since it regulates freshwater macrophyte metabolism and growth (Madsen et al, 2001).…”