Absorption Spectra of Rhodamine 6G by Slab Optical Waveguide Spectroscopy

Abstract

Slab optical waveguide (SOWG) was employed for obtaining absorption spectra of rhodamine 6G (Rh6G) with the concentration of 1 μmol/dm3. It was found that Rh6G existed as a monomer and dimer on SOWG surfaces. On the SOWG surfaces treated with octadecylsilane only the band assigned to a monomer of Rh6G was observed.

Adsorption Behavior of Cytochrome c, Myoglobin and Hemoglobin in a Quartz Surface Probed Using Slab Optical Waveguide (SOWG) Spectroscopy

“…4,[8][9][10][11][12] An Xe150W Lamp Unit (SIC, Japan) was used as the light source. A cell composed of a quartz plate was mounted on a flat silicone rubber sheet with a hollow center, as in Fig.…”

“…2,3 The reported nomenclature in the literature include slab optical waveguide (SOWG) spectroscopy, 4-17 the integrated optical waveguideattenuated total reflection technique (IOW-ATR) [18][19][20][21][22][23][24][25][26][27] and optical waveguide lightmode spectroscopy (OWLS). 1,[28][29][30][31][32][33][34][35][36][37][38] These techniques have been successfully used in real-time studies on the adsorption of dye molecules 11,12,[14][15][16] and proteins 4,8,[18][19][20][21][22][23][24][25][28][29][30][31][32][33][34][35][36][37]…”

“…Since the interacting photons come from the waveguiding surface, one advantage of ITR-based spectroscopy is its ability to interact mostly with the adsorbed molecules and much less with those in the bulk solution. Some of the research groups that are actively involved in studying protein adsorption on surfaces based on ITR are Jeremy Ramsden's group in Basel, Switzerland, 28-37 Scott Saavedra's group in Arizona, [18][19][20][21][22][23][24][25][26][27] Paul R. Van Tassel's group in Wayne, USA 38,40 and some research groups in Japan [4][5][6][7][8][9][10][11][12][13][14][15][16][17]39 and elsewhere. 41,42 In this work we investigated, under various solution conditions, the adsorption behavior of hemoglobin, myoglobin and cytochrome c in a bare quartz surface.…”

Dynamic Analysis of Adsorption-Desorption Equilibrium of Sublimed Aromatic Molecules Using Polarized Optical Waveguide Spectroscopy

“…3,4 Optical waveguide (OWG) spectrometry is a method that uses evanescent waves generated at the total reflection of incident light at the waveguide surface. [5][6][7][8][9][10][11] The penetration depth of the evanescent waves is almost the same as the wavelength of the light used. Since the evanescent wave exponentially decreases from the interface, this technique detects the electronic state of molecules only at the surface.…”

Time-Resolved Optical Waveguide Spectroscopy for Studying Protein Adsorption Kinetics

“…For several years now, our group has been developing this technique and tried a number of ways to investigate interfacial phenomena involving proteins and dye molecules, particularly adsorption processes, by measuring the transmission and absorbance spectra of the reflected light using various waveguide materials. [6][7][8][9][10][11][12][13][14] Recently, we have developed a cell arrangement wherein a polychromatic light is transmitted within the waveguide using a glycerol drop in lieu of conventional prism or grating couplers. 6,7) We have combined this novel cell arrangement with the high-speed photonic charge-coupled device to observe the adsorption behavior of proteins right after solution-surface contact.…”