Absorption spectrum analysis of dentine sialophosphoprotein (DSPP) in orthodontic patient

Zain, Mohd Norzaliman Mohd; Yusof, Zalhan Md; Yazid, Farinawati; Ashari, Asma; Wong, Koh-Ping; Lee, W. J.; Tan, Kok-Keong; Ariffin, Shahrul Hisham Zainal; Wahab, Rohaya Megat Abdul

doi:10.1063/1.5142099

Cited by 5 publications

(14 citation statements)

References 13 publications

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“…The penetration of light radiation through dentin specimens depends on tissue's optical properties, chromophores such as melanin, hemoglobin, and water, the technical speci cations of the device, and the operation method [37,41]. Furthermore, another study considered the increase in transfer rate in enamel to dentin with differences in refractive indices coe cients related to light extensions [31].…”

Section: Discussionmentioning

confidence: 99%

“…Dogandzhiyska et al [37] measured the absorption and penetration of light in the wavelength range 350-1000 nm on dentin specimens with a thickness of 1 mm prepared from above the roof of the pulp chamber perpendicular to the longitudinal axis of the tooth. They reported that the highest tissue uptake in radiation was observed in the blue spectral region and the lowest in the infrared spectral range.…”

Section: Discussionmentioning

confidence: 99%

“…The estimation of beam absorption in dentin or any other tissue is a function of the frequency and wavelength of that light [34][35][36]. However, few studies have investigated the patterns of laser's beam absorption in dentin and, particularly the depth of penetration and its effect on amount of absorption and distribution [23,31,37].…”

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confidence: 99%

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The Effect of Beam Direction on Absorption and Transmission of Ultraviolet to Infra-red Wave-length in Three Different Dentin Thicknesses

Eslami¹,

Kazeminejad²,

Karimian³

2021

Preprint

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BackgroundsLasers and optics have extensively been used in dental procedures in recent years, so realizing the optical properties of the tooth represents a milestone in its successful applications. the aim of this study was to compare the absorption and transmission of applied wavelengths in 190-1100 nm range in various dentin thicknesses and the effect of changing the direction of beam emission in dentinal tubules.MethodsFifteen dentin specimens with a thickness of 300, 600, and 1000 µm and five specimens from each thickness were prepared by a transverse incision at the upper pulpal roof area of the human molars.Considering the Corono-apical and Apico-coronal direction, we measured the absorption and transmission of parallel light beams perpendicular to the dentin specimens in various thicknesses and two directions using a UV/ Visible spectrometer.ResultsThe ultraviolet wavelength's absorption rate was significantly higher than visible and infrared light irradiation from both directions in three thicknesses (p-value<0.001). Additionally, The radiation beam displacement had no significant differences in the absorption and transmission of ultraviolet, visible, and infrared light in any of the three thicknesses (p-value>0.05).ConclusionAccording to the results, the change in the beam direction during irradiation does not cause a significant difference in light absorption. Furthermore, the results are expected to develop a suitable method for evaluating the trans-dental performance of different optical parameters for diagnostic purposes in the dental tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Effect of Beam Direction on Absorption and Transmission of Ultraviolet to Infra-red Wave-length in Three Different Dentin Thicknesses

Eslami¹,

Kazeminejad²,

Karimian³

2021

Preprint

View full text Add to dashboard Cite

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“…A total of 372 articles were initially identified, duplicate publications were removed, and the inclusion and exclusion criteria were applied, resulting in 20 articles found being included in the final review (Figure 2). 2,5,7,9,12,13,15,16,[19][20][21][22][23][24][25][26][27][28][29][30]…”

Section: Study Selectionmentioning

confidence: 99%

Biomarkers in External Apical Root Resorption: An Evidence-based Scoping Review in Biofluids

Kapoor¹,

Chowdhry²,

Bagga³

et al. 2022

Rambam Maimonides Med J

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Background: External apical root resorption (EARR), an unwanted sequela of orthodontic treatment, is difficult to diagnose radiographically. Hence, the current scoping review was planned to generate critical evidence related to biomarkers in oral fluids, i.e. gingival crevicular fluid (GCF), saliva, and blood, of patients showing root resorption, compared to no-resorption or physiologic resorption. Methods: A literature search was conducted in major databases along with a manual search of relevant articles in the library, and further search from references of the related articles in March 2021. The initial search was subjected to strict inclusion and exclusion criteria according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Results: Following PRISMA guidelines, 20 studies were included in the final review. The studies included human clinical trials and cross-sectional and prospective studies with/without control groups with no date/ language restriction. Various biomarkers identified in EARR included dentinal proteins, enzymes, cytokines, and salivary proteins. Severe resorption had higher dentin sialoprotein (DSP) and resorption protein concentrations as well as lower granulocyte-macrophage colony-stimulating factor (GM-CSF) as compared with mild resorption. Increased DSP and dentin phosphophoryn (DPP) expression was found in physiologic resorption. Compared to controls, resorbed teeth showed a higher receptor activator of nuclear factor kappa B ligand/ osteoprotegerin (RANKL/OPG) ratio. In contrast, levels of anti-resorptive mediators (IL-1RA, IL-4) was significantly decreased. Differences in force levels (150 g and 100 g) showed no difference in resorption, but a significant rise in biomarkers (aspartate transaminase [AST] and alkaline phosphatase [ALP]) for 150 g force. Moderate to severe resorption in young patients showed a rise in specific salivary proteins, requiring further validation. Limitations of the studies were heterogeneity in study design, biomarker collection, sample selection, and confounding inflammatory conditions. Conclusions: Various biomarkers in biofluids indicate active resorption, while resorption severity was associated with DSP and GM-CSF in GCF, and a few salivary proteins. However, a robust study design in the future is mandated.

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“…However, ELISA lacks accuracy and speci city in detecting the sample at trace level and depends only on an absorption of a single wavelength. Multi wavelength analysis of biological markers spectrum proven to give a conclusive result [10][11]. The objective of this study is to investigate the univariate and multivariate analysis of the ELISA spectrum trace samples.…”

Section: Introductionmentioning

confidence: 99%

Multivariate Versus Univariate Spectrum Analysis of Dentine Sialophosphoprotein (DSPP) for Root Resorption Prediction: A Clinical Trial

Zain¹,

Yusof²,

Basri³

et al. 2021

Preprint

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Background: A force applied during orthodontic treatment induces inflammation to root area and lead to root resorption known as Orthodontically Induced Inflammatory Root Resorption (OIIRR). Dentine sialophosphoprotein (DSPP) is one of the most abundant non-collagenous protein in dentine that was released into gingival crevicular fluid (GCF) during OIIRR. The aim of this research is to compare DSPP detection using the univariate and multivariate analysis in predicting classification level of root resorption. Methods: The subjects for this study consisted of 30 patients in 3 group classified as normal, mild and severe groups of OIIRR. The GCF samples were taken from upper permanent central incisors in the normal and mild group while the upper primary second molars in the severe group. The DSPP qualitative detection limit was determined by analyzing the whole absorption spectrum utilizing multivariate analysis embedded with different preprocessing method. The multivariate analysis represents the multi-wavelength spectrum while univariate analyzes the absorption of a single wavelength. Results: The results showed that the multivariate analysis technique using Partial Least Square-Discriminate Analysis (PLS-DA) with the preprocess method has successfully improved in classification prediction for the normal and mild group at 0.88 percent accuracy. The multivariate using PLS-DA algorithm with Mean Center preprocess method was able to predict normal and mild tooth resorption classes better than the univariate analysis. The classification parameters have improved in term of the specificity, precision and accuracy. Conclusion: Therefore, the multivariate analysis helps to predict an early detection of tooth resorption complimenting the sensitivity of the univariate analysis.

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Absorption spectrum analysis of dentine sialophosphoprotein (DSPP) in orthodontic patient

Cited by 5 publications

References 13 publications

The Effect of Beam Direction on Absorption and Transmission of Ultraviolet to Infra-red Wave-length in Three Different Dentin Thicknesses

The Effect of Beam Direction on Absorption and Transmission of Ultraviolet to Infra-red Wave-length in Three Different Dentin Thicknesses

Biomarkers in External Apical Root Resorption: An Evidence-based Scoping Review in Biofluids

Multivariate Versus Univariate Spectrum Analysis of Dentine Sialophosphoprotein (DSPP) for Root Resorption Prediction: A Clinical Trial

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