Abstract 414: Lipidomic Of Platelet Concentrate Dedicated For Transfusion

Duchez, Anne‐Claire; Fauteux‐Daniel, Sébastien; Sut, Caroline; Ebermeyer, Theo; Arthaud, Charles‐Antoine; Eyraud, Marielle; Prier, Amélie; Audoux, Estelle; BERTRAND-MICHEL, Justine; Payrastre, Bernard; Garraud, Olivier; Boilard, Éric; Hamzeh‐Cognasse, Hind; Cognasse, F.

doi:10.1161/atvb.42.suppl_1.414

Cited by 1 publication

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have assessed the lipid content of platelet concentrates (8,(25)(26)(27) or, in the case of a severe adverse reaction, a few lipids (28), but this is not combined with an extensive lipid mediator assessment or linked with an adverse reaction post-PC transfusion. Our study highlights bioactive lipids in SDA-PC during storage and some lipids that could shed light on the onset of adverse reaction in recipients (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

“…The SDA-PC lipid mediator content during storage was investigated previously (8,42). However, the authors linked the lipid mediators with EV contained in the PC.…”

Section: Discussionmentioning

confidence: 99%

“…SDA-PCs were collected as described above (8,17). Briefly, blood was collected on ACD-A using Trima, a continuous-flow cell separator (Gambro BCT, Lakewood, CO, USA).…”

Section: Sample Preparationmentioning

confidence: 99%

“…These reactions are induced by biomolecules such as cytokines and lipids as well as storage (time, temperature, etc.). PC processing also plays a significant role in terms of proteomic changes that can escalate into adverse reactions (7), although it did not alter the lipidome (8).…”

Section: Introductionmentioning

confidence: 96%

See 3 more Smart Citations

Bioactive lipids as biomarkers of adverse reactions associated with apheresis platelet concentrate transfusion

et al. 2023

Self Cite

View full text Add to dashboard Cite

Platelet concentrate (PC) transfusion seeks to provide haemostasis in patients presenting severe central thrombocytopenia or severe bleeding. PCs may induce adverse reactions (AR) that can occasionally be severe (SAR). PCs contain active biomolecules such as cytokines and lipid mediators. The processing and storage of PCs creates so-called structural and biochemical storage lesions that accumulate when blood products reach their shelf life. We sought to investigate lipid mediators as bioactive molecules of interest during storage and review associations with adverse reactions post-transfusion. To facilitate understanding, we focused on single donor apheresis (SDA) PCs with approximately 31.8% of PCs being delivered in our setting. Indeed, pooled PCs are the most widely transfused products, but the study of a single donor lipid mediator is easier to interpret. We are investigating key lipid mediators involved in AR. Adverse reactions were closely monitored in accordance with current national and regional haemovigilance protocols. Residual PCs were analysed post-transfusion in a series of observations, both with and without severe reactions in recipients. A decrease in the lysophosphatidylcholine species to produce the lysophosphatidic acid species has been observed during storage and in the case of AR. Lysophosphatidic acid increased with primarily platelet-inhibitor lipids. Anti-inflammatory platelet-induced inhibition lipids were weakly expressed in cases of severe adverse reactions. We therefore propose that a decrease in lysophosphatidylcholine and an increase in lysophosphatidic acid can prospectively predict serious adverse transfusion reactions.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The SDA-PC lipid mediator content during storage was investigated previously (8,42). However, the authors linked the lipid mediators with EV contained in the PC.…”

Section: Discussionmentioning

confidence: 99%

“…SDA-PCs were collected as described above (8,17). Briefly, blood was collected on ACD-A using Trima, a continuous-flow cell separator (Gambro BCT, Lakewood, CO, USA).…”

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations