Abstract 4208: Inhibition of proliferation and induction of apoptosis by a novel phytobiological mixture against breast cancer cell lines

Abstract

Breast cancer remains a leading cause of women’s death worldwide with progressive emergence of resistance towards approved chemotherapies. For this reason, new complementarytreatments have been sought. One of those includethe use of a phytobiological mixture against different types of malignancies, including but not limited to breast cancer. Herein, we investigated the anti-proliferative effects of a novel phytobiological mixture (PB)against two breast cancer cell lines :MDA-MB-231 and MCF-7.The PB mixture is composed of the following: quercetin, resveratrol, cruciferex, epigallocatechin-3-gallate and curcumin at different proportions. Theanti-proliferative and pro-apoptotic effects of PB were evaluated at non-cytotoxic concentrations as determined usingthe Sulfa-Rhodamine B assay (SRB).The effects of PB on the induction of apoptosis were valuated using Annexin-V and flow cytometry to measure the resultingDNA fragments. The results showed that at 72hrs, PB had cytotoxic effects against MDA-MB-231 and MCF-7 cells with an IC50’s of 25.8±5.4 µg/ml and 56.9±4.2 µg/ml, respectively. Exposure of MDA-MB-231 to PB for 72hrs resulted in the induction of apoptosis in a dose-dependent manner. PB concentrations of 10 µg/ml and 30 µg/ml, resulted in early programmed cell death of 9.1±1.5% and 22.1±1.8% respectively. Exposure to PB (10 µg/ml for 24 hrs) resulted in mild cell cycle arrest at the G2/M phase20.3±0.3% ;while higher dosesshowed a significant cell cyclearrest at theS-phaseas compared to the control untreated cells (p<0.001). However, exposure to PB for 48 hrs at both doses,10 µg/ml and30 µg/ml,resulted in a significant cell cycle arrest at the S-phase 25.3±1.6% and 20.5± 0.4%respectivelyas compared to the control untreated cells 12.4±1.5% (p<0.001). In conclusion, PB was found to have a promising anti-proliferative and pro-apoptotic effect in breast cancer cell lines. Further studies are needed to address the underlying mechanism of action of PB against breast cancer cell lines. Citation Format: Afnan A. Alqarni, Aliaa A. Alamoudi, Ghada M. Ajabnoor, Ahmed M. Al-Abd, Aleksandra Niedwiecki, Matthias Rath, Steve Harakeh. Inhibition of proliferation and induction of apoptosis by a novel phytobiological mixture against breast cancer cell lines [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4208. doi:10.1158/1538-7445.AM2017-4208

Enhancement of Annexin V in response to combination of epigallocatechin gallate and quercetin as a potent arrest the cell cycle of colorectal cancer

“…The annexin V/PI assay with flow cytometry showed that PB induced the pro-apoptotic effect in HCT116 cells in a concentration dependent manner at 24 h. At the highest concentration of 150 ug/mL, the percentage of annexin V positive cells stood at 28.55%. Our findings are in accordance with the previous studies that reported that the exposure of MDA-MB-231 breast cancer cells to PB resulted in the induction of apoptosis in a concentration-dependent manner (Alqarni et al, 2017).…”

“…It can be inferred that at higher concentrations, PB may have arrested the replication of DNA and hence prevented the cells to enter into the G2 phase leading to the decreased cell division and overall inhibitory effect on the cancer cell growth. Previous studies have also reported that exposure of MDA-MB-231 to concentrations of PB of 10 ug/mL and 30 ug/mL resulted in a significant cell cycle arrest at the G1/S phase with a population of 25.3 ± 1.6% and 20.5 ± 0.4%, respectively, as compared to the control untreated cells 12.4±1.5% (p<0.001) (Alqarni et al, 2017). However, further molecular studies at the protein and gene level are necessary which can enable us to arrive at a concrete decision.…”

“…The chemical composition of the PB contained. Previous studies have shown the anti-proliferative and anti-cancerous potential of PB in breast, sarcoma, skin and head and neck cancer cell lines (Alqarni et al, 2017;Niedzwiecki et al, 2016;Roomi et al, 2011Roomi et al, , 2015.…”