Abstract C25: Aspiration liquid biopsy (ALB) of pancreatic cancer (PC)

Abstract

Introduction: Early detection of PC is the main clue for improving results of treatment. Liquid biopsy (LqB) is the most promising method of detection of disease in a screening context, but selection of biologic material for that is not a resolved problem. Pancreatic juice is the highly specific substance for that aim. However, its collection and analysis are associated with many technical difficulties. Materials and Methods: For pancreatic juice collection we developed a method of ALB using a proprietory pancreatodigestive stent. This stent allows for active aspiration of pancreatic juice. We had recruited 15 patients with verified PC before treatment. Placement of the stent was performed by endoscopy and followed by collecting of pancreatic juice in sterile container at 4o C. After collecting sufficiently large volume of the material, we centrifugated it and made cell-block for cytologic examination. Results: ALB was performed in all 15 cases without any complications. Placement and removal of the stent was atraumatic and without technical problems. However, only in 1 sample out of 15 did we detect atypical cells using standard cytologic examination. Conclusion: We present new methodology for collecting biomaterial potentially useful for the early diagnosis of pancreatic cancer. This method is simple and can be easily replicated. Its application does not require high-quality specialized medical centers and personnel and can be performed in standard gastroenterologic clinics. Collected samples can be easily stored. Furthermore, in addition to cells it allows to obtain the liquid fraction of pancreatic juice that could be used for the detection of tumor DNA. Citation Format: Aleksei Kashintsev, Nikolai Kokhanenko, Evgeniy Imyanitov, Vitaliy Proutski, Oleg Granstrem, Lyudmila Solovieva, Konstantin Medvedev, Konstantin Pavelets, Sergey Anisimov. Aspiration liquid biopsy (ALB) of pancreatic cancer (PC) [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer: Advances in Science and Clinical Care; 2019 Sept 6-9; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2019;79(24 Suppl):Abstract nr C25.

Early selective enteral feeding in treatment of acute pancreatitis: A case report

“…However, the method of administering nutrition, whether via oral, gastric, or intestinal route, remains unresolved[ 4 , 5 ]. A new treatment method utilizing the novel catheter PandiCath ® has been developed[ 6 , 7 ], offering several potential treatment mechanisms, including selective enteral feeding. The device temporarily isolates and disconnects the duodenum from the stomach and proximal jejunum, preventing acidification of duodenal contents as well as reflux of bacterial flora from the small intestine to the duodenum.…”